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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
21/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMARGO, L. S. de A.; BOITE, M. C.; WOHLRES-VIANA, S.; MOTA, G. B.; SERAPIÃO, R. V.; SÁ, W. F. de; VIANA, J. H. M.; NOGUEIRA, L. A. G. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARIANA CORTES BOITE, FIOCRUZ; SABINE WOHLRES-VIANA, UFJF; GUSTAVO BRUNO MOTA, UNB; RAQUEL VARELA SERAPIÃO, PESAGRO; WANDERLEI FERREIRA DE SÁ, Pesquisador aposentado do CNPGL; JOAO HENRIQUE MOREIRA VIANA, CNPGL; LUIZ ALTAMIRO GARCIA NOGUEIRA, UFF. |
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Título: |
Osmotic challenge and expression of aquaporin 3 and Na/K ATPase genes in bovine embryos produced in vitro. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Cryobiology, v. 63, n. 3, p. 256-262, 2011. |
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DOI: |
https://doi.org/10.1016/j.cryobiol.2011.09.135 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-warmed embryos (57.9%) than for their fresh counterparts (84.6%). There was no difference on expression of ATPase1 gene but lower (P<0.01) amount of Aqp3 transcripts was found in the vitrified-warmed embryos. In conclusion, embryo ability to undergo shrinkage and swelling is influenced by medium used in a co-culture system and by embryo stage. Rehydrating ability of embryos after exposure to NaCl hypertonic medium is not associated with variations on expression of Aqp3 and ATPase1 genes, but the vitrification can alter gene expression of in vitro-fertilized bovine embryos produced in a co-culture system. MenosThe aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-... Mostrar Tudo |
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Palavras-Chave: |
Embryo. |
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Thesaurus Nal: |
cryopreservation; osmosis; vitrification. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02924naa a2200265 a 4500
001 1910654
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.cryobiol.2011.09.135$2DOI
100 1 $aCAMARGO, L. S. de A.
245 $aOsmotic challenge and expression of aquaporin 3 and Na/K ATPase genes in bovine embryos produced in vitro.$h[electronic resource]
260 $c2011
520 $aThe aim of this study was to evaluate the effect of culture media and stage of development in the osmotic ability of in vitro-fertilized bovine embryos and the expression of aquaporin 3 (Aqp3) and Na/K ATPase isoform 1 (ATPAse1) genes in embryos (i) with different ability to undergo rehydration and (ii) following vitrification. In experiment 1, in vitro fertilized presumptive zygotes were co-cultured in SOFaac or modified CR2aa medium and embryos at blastocyst and expanded blastocyst stages at day 7 post-insemination were exposed to NaCl hypertonic medium (900 mOsm) for 5 min following 120 min of culture in isotonic medium in order to evaluate dehydration and rehydration, respectively. No difference (P>0.05) on blastocyst rate was found between CR2aa and SOFaac medium but embryos co-cultured in SOFaac medium underwent greater (P<0.05) dehydration. Embryos at expanded blastocyst stage underwent greater dehydration but slower rehydration than embryos at blastocysts stage (P<0.05). In the experiment 2, the amount of Aqp3 and ATPase1 transcripts were quantified in blastocysts with high or low rehydration after exposure to hypertonic medium. No difference (P>0.05) on relative amount of transcripts was found in either genes. In the experiment 3, expanded blastocysts produced in a co-culture system were vitrified, warmed and then cultured for 72 h for analysis of embryo survival and amount of Aqp3 and ATPase1 transcripts. Lower (P<0.05) embryo survival rate was found for vitrified-warmed embryos (57.9%) than for their fresh counterparts (84.6%). There was no difference on expression of ATPase1 gene but lower (P<0.01) amount of Aqp3 transcripts was found in the vitrified-warmed embryos. In conclusion, embryo ability to undergo shrinkage and swelling is influenced by medium used in a co-culture system and by embryo stage. Rehydrating ability of embryos after exposure to NaCl hypertonic medium is not associated with variations on expression of Aqp3 and ATPase1 genes, but the vitrification can alter gene expression of in vitro-fertilized bovine embryos produced in a co-culture system.
650 $acryopreservation
650 $aosmosis
650 $avitrification
653 $aEmbryo
700 1 $aBOITE, M. C.
700 1 $aWOHLRES-VIANA, S.
700 1 $aMOTA, G. B.
700 1 $aSERAPIÃO, R. V.
700 1 $aSÁ, W. F. de
700 1 $aVIANA, J. H. M.
700 1 $aNOGUEIRA, L. A. G.
773 $tCryobiology$gv. 63, n. 3, p. 256-262, 2011.
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Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 34 | |
| 4. |  | SILVA, N. G. B.; SOUZA, A. V. de; SOUZA, D. D.; OLIVEIRA, F. J. V. Tipo de estaca e concentração de ácido indol butírico no enraizamento de alecrim da chapada. Horticultura Brasileira, v. 30, n. 2, p. S6016-S6022, jul. 2012. 1 CD-ROM. Suplemento. Edição dos Anais do 52 Congresso Brasileiro de Olericultura, Salvador, jul. 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 5. | | SOUZA, M. D. de; SANTOS, M. C.; SOUZA, D. D. de; SOUZA, A. V. de; SANTANA, J. R. F. de. Estabelecimento in vitro de baraúna sob diferentes concentrações de BAP e AIA. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 9., 2014, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2014. p. 57-61. (Embrapa Semiárido. Documentos, 261).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 8. | | SOUZA, A. V. de; SILVA, F. P.; SILVA, N. G. B.; SOUZA, D. D. de; OLIVEIRA, F. J. V. Influência do tempo de exposição em ácido indol butírico no enraizamento de estacas de velame. Horticultura Brasileira, Brasília, DF, v. 30, n. 2, p. S5997-S6002, jul. 2012. 1 CD-ROM. Suplemento. Edição dos Anais do 52 Congresso Brasileiro de Olericultura, Salvador, jul. 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 16. | | SANTOS, U. S. dos; SOUZA, D. D. de; SILVA, F. P. da; GUIMARÃES, N. B.; SOUZA, A. V. de. Cultivo do Alecrim-do-Mato no Vale do São Francisco em função da adubação organo-mineral em condições de estresse hídrico. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 7.; JORNADA DE INICIAÇÃO CIENTÍFICA DA FACEPE/UNIVASF, 1., 2012, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2012. p. 165-170, 1 CD-ROM. (Embrapa Semiárido. Documentos, 248).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 17. | | SOUZA, A. V. de; SOUZA, D. D.; SILVA, F. P.; SANTOS, U. S.; SANTOS, M. C.; OLIVEIRA, F, J. V. Cultivo do alecrim do mato no Vale do São Francisco em função da adubação organo-mineral. Horticultura Brasileira, Brasília, DF, v. 30, n. 2, p. S6010-S6015, jul. 2012. 1 CD-ROM. Suplemento. Edição dos Anais do 52 Congresso Brasileiro de Olericultura, Salvador, jul. 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 18. | | SOUZA, A. V. de; SANTOS, U. S.; SILVA, F. P.; SOUZA, D. D.; SANTOS, M. C.; OLIVEIRA, F. J. V. Produção e rendimento de óleo essencial de alecrim de tabuleiro em função da adubação orgânica. Horticultura Brasileira, Brasília, DF, v. 30, n. 2, p. S5985-S5990, jul. 2012. 1 CD-ROM. Suplemento. Edição dos Anais do 52 Congresso Brasileiro de Olericultura, Salvador, jul. 2012,| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 20. | | SOUZA, D. D. de; SILVA, N. B. G. da; OLIVEIRA, F. J. V. de; SOUZA, A. V. de. Produção de mudas de umburana-de-cheiro em diferentes recipientes e substratos. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 7.; JORNADA DE INICIAÇÃO CIENTÍFICA DA FACEPE/UNIVASF, 1., 2012, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2012. p. 171-176. 1 CD-ROM. (Embrapa Semiárido. Documentos, 248).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 34 | |
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