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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
21/12/2011 |
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Data da última atualização: |
26/03/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARDOSO, J. D.; HUNGRIA, M.; ANDRADE, D. S. |
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Afiliação: |
JUSCÉLIO DONIZETE CARDOSO, UEL; MARIANGELA HUNGRIA DA CUNHA, CNPSO; DIVA S. ANDRADE, IAPAR. |
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Título: |
Polyphasic approach for the characterization of rhizobial symbionts effective in fixing N2 with common bean (Phaseolus vulgaris L.). |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Applied Microbiology and Biotechnology, v. 93, n. 5, p. 2035-2049, mar. 2012. |
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DOI: |
10.1007/s00253-011-3708-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Common bean (Phaseolus vulgaris L.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. The aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of Arachis hypogaea, Mucuna pruriens, and soils from various Brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. Forty-five elite strains were analyzed for symbiotic properties (nodulation, plant-growth, and nitrogen-fixation parameters) under greenhouse conditions in pots containing non-sterile soil, and variation in symbiotic performance was observed. Elite strains were also characterized in relation to morphophysiological properties, genetic profiles of reppolymerase chain reaction (PCR; BOX), and restriction fragment length polymorphism (RFLP)-PCR of the 16S rRNA. Sequence analyses of the 16S rRNA were obtained for 17 strains representative of the main groups resulting from all previous analyses. One of the most effective strains, IPR-Pv 2604, was clustered with Rhizobium tropici, whereas strain IPR-Pv 583, showing lower effectiveness in fixing N2, was clustered with Herbaspirillum lusitanum. Surprisingly, effective strains were clustered with unusual symbiotic genera/species, including Leifsonia xyli, Stenotrophomonas maltophilia, Burkholderia, and Enterobacter. Some strains recognized in this study were outstanding in their nitrogen-fixing capacity and therefore, show high biotechnological potential for use in commercial inoculants. MenosCommon bean (Phaseolus vulgaris L.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. The aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of Arachis hypogaea, Mucuna pruriens, and soils from various Brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. Forty-five elite strains were analyzed for symbiotic properties (nodulation, plant-growth, and nitrogen-fixation parameters) under greenhouse conditions in pots containing non-sterile soil, and variation in symbiotic performance was observed. Elite strains were also characterized in relation to morphophysiological properties, genetic profiles of reppolymerase chain reaction (PCR; BOX), and restriction fragment length polymorphism (RFLP)-PCR of the 16S rRNA. Sequence analyses of the 16S rRNA were obtained for 17 strains representative of the main groups resulting from all previous analyses. One of the most effective strains, IPR-Pv 2604, was clustered with Rhizobium tropici, whereas strain IPR-Pv 583, showing lower effectiveness in fixing N2, was clustered with Herbaspirillum lusitanum. Surprisingly, effective strains were clustered with unusual symbiotic genera/species, including Leifsonia xyli, Stenotrophomonas maltophilia, Burkholderia, and Enterobacter. Some strains recognized in this study wer... Mostrar Tudo |
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Palavras-Chave: |
Fixação biológica de nitrogênio. |
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Thesagro: |
Feijão; Fixação de Nitrogênio. |
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Thesaurus Nal: |
Beans; Nitrogen fixation. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02368naa a2200217 a 4500
001 1910587
005 2025-03-26
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1007/s00253-011-3708-2$2DOI
100 1 $aCARDOSO, J. D.
245 $aPolyphasic approach for the characterization of rhizobial symbionts effective in fixing N2 with common bean (Phaseolus vulgaris L.).$h[electronic resource]
260 $c2012
520 $aCommon bean (Phaseolus vulgaris L.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. The aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of Arachis hypogaea, Mucuna pruriens, and soils from various Brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. Forty-five elite strains were analyzed for symbiotic properties (nodulation, plant-growth, and nitrogen-fixation parameters) under greenhouse conditions in pots containing non-sterile soil, and variation in symbiotic performance was observed. Elite strains were also characterized in relation to morphophysiological properties, genetic profiles of reppolymerase chain reaction (PCR; BOX), and restriction fragment length polymorphism (RFLP)-PCR of the 16S rRNA. Sequence analyses of the 16S rRNA were obtained for 17 strains representative of the main groups resulting from all previous analyses. One of the most effective strains, IPR-Pv 2604, was clustered with Rhizobium tropici, whereas strain IPR-Pv 583, showing lower effectiveness in fixing N2, was clustered with Herbaspirillum lusitanum. Surprisingly, effective strains were clustered with unusual symbiotic genera/species, including Leifsonia xyli, Stenotrophomonas maltophilia, Burkholderia, and Enterobacter. Some strains recognized in this study were outstanding in their nitrogen-fixing capacity and therefore, show high biotechnological potential for use in commercial inoculants.
650 $aBeans
650 $aNitrogen fixation
650 $aFeijão
650 $aFixação de Nitrogênio
653 $aFixação biológica de nitrogênio
700 1 $aHUNGRIA, M.
700 1 $aANDRADE, D. S.
773 $tApplied Microbiology and Biotechnology$gv. 93, n. 5, p. 2035-2049, mar. 2012.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
10/09/1999 |
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Data da última atualização: |
12/03/2025 |
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Autoria: |
MOREIRA, J. R.; KLAUTAU-GUIMARAES, N.; PILLA, E. J. S.; CONTEL, E. P. B.; DE BEM, A. R. |
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Afiliação: |
EMBRAPA-CENARGEN. |
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Título: |
Estudo preliminar da variabilidade genética do lobo-guará (Chrysocyon brachyurus). |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Brasilia: EMBRAPA-CENARGEN, 1998 |
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Páginas: |
10p. |
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Série: |
(EMBRAPA-CENARGEN. Comunicado Tecnico, 30) |
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Idioma: |
Português |
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Conteúdo: |
Este comunicado de dados preliminares visa avaliar a diversidade genética de populações de lobos-guarás nos estados de Goiás, Minas Gerais, São Paulo e Distrito Federal e comparar a diversidade genética de regiões submetidas a diferentes pressões antrópicas. |
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Palavras-Chave: |
Genetic variability; Lobo guara; Lobo-guara; Variabilidade genetica. |
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Thesagro: |
Genética. |
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Thesaurus NAL: |
Chrysocyon brachyurus; genetics; wolves. |
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Categoria do assunto: |
-- |
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URL: |
https://www.infoteca.cnptia.embrapa.br/infoteca/bitstream/doc/179556/1/Comunicado-tecnico-30-CENARGEN.pdf
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Marc: |
LEADER 01042nam a2200277 a 4500
001 1179556
005 2025-03-12
008 1998 bl uuuu u0uu1 u #d
100 1 $aMOREIRA, J. R.
245 $aEstudo preliminar da variabilidade genética do lobo-guará (Chrysocyon brachyurus).
260 $aBrasilia: EMBRAPA-CENARGEN$c1998
300 $a10p.
490 $a(EMBRAPA-CENARGEN. Comunicado Tecnico, 30)
520 $aEste comunicado de dados preliminares visa avaliar a diversidade genética de populações de lobos-guarás nos estados de Goiás, Minas Gerais, São Paulo e Distrito Federal e comparar a diversidade genética de regiões submetidas a diferentes pressões antrópicas.
650 $aChrysocyon brachyurus
650 $agenetics
650 $awolves
650 $aGenética
653 $aGenetic variability
653 $aLobo guara
653 $aLobo-guara
653 $aVariabilidade genetica
700 1 $aKLAUTAU-GUIMARAES, N.
700 1 $aPILLA, E. J. S.
700 1 $aCONTEL, E. P. B.
700 1 $aDE BEM, A. R.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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