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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
16/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUIMARAES, A. S.; DORNELES, E. M. S.; ANDRADE, G. I.; LAGE, A. P.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. |
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Afiliação: |
ALESSANDRO DE SA GUIMARAES, CNPGL; ELAINE MARIA SELES DORNELES, UFMG; GIOVANNA IVO ANDRADE, UFMG; ANDREY PEREIRA LAGE, UFMG; GEPOC; ANDERSON MIYOSHI, UFMG; VASCO AZEVEDO, UFMG; GEPOC; AURORA MARIA GUIMARÃES GOUVEIA, UFMG; GEPOC; MARCOS BRYAN HEINEMANN, UFMG; GEPOC. |
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Título: |
Molecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Veterinary Microbiology, v. 153, n. 3/4, p. 299-306, 2011. |
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DOI: |
https://doi.org/10.1016/j.vetmic.2011.06.002 |
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Idioma: |
Inglês |
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Conteúdo: |
Caseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates. MenosCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10... Mostrar Tudo |
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Palavras-Chave: |
ERIC-PCR; RAPD. |
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Thesagro: |
Corynebacterium Pseudotuberculosis. |
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Thesaurus Nal: |
caseous lymphadenitis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02507naa a2200265 a 4500
001 1910089
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.vetmic.2011.06.002$2DOI
100 1 $aGUIMARAES, A. S.
245 $aMolecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR.$h[electronic resource]
260 $c2011
520 $aCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates.
650 $acaseous lymphadenitis
650 $aCorynebacterium Pseudotuberculosis
653 $aERIC-PCR
653 $aRAPD
700 1 $aDORNELES, E. M. S.
700 1 $aANDRADE, G. I.
700 1 $aLAGE, A. P.
700 1 $aMIYOSHI, A.
700 1 $aAZEVEDO, V.
700 1 $aGOUVEIA, A. M. G.
700 1 $aHEINEMANN, M. B.
773 $tVeterinary Microbiology$gv. 153, n. 3/4, p. 299-306, 2011.
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Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 12 | |
| 2. |  | MENDES, A. C. S.; DUARTE, M. S.; RAMOS, A. K. B.; FERNANDES, F. D.; KARIA, C. T.; CARVALHO, M. A. Análise da produtividade de sementes de acessos selecionados de Stylosanthes guianensis. In: INTERNATIONAL SYMPOSIUM ON FORAGE BREEDING, 3., 2011, Bonito. Breeding forages for climate change adaptation and mitigation - eco-efficient animal production: proceedings... [Campo Grande: Embrapa Gado de Corte, 2011]. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 3. | | DUARTE, M. S.; RAMOS, A. K. B.; FERNANDES, F. D.; KARIA, C. T.; CARVALHO, M. A.; SANTOS, F. S.; AVIANI, D. M. Descritores morfológicos para realização de ensaios de distinguibilidade, estabilidade e homogeneidade em cultivares de Brachiaria. In: INTERNATIONAL SYMPOSIUM ON FORAGE BREEDING, 3., 2011, Bonito. Breeding forages for climate change adaptation and mitigation - eco-efficient animal production: proceedings... [Campo Grande: Embrapa Gado de Corte, 2011]. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 6. | | MENEZES, C. A. DE M.; DUARTE, M. S.; TEIXEIRA, I. N.; CAVALCANTE, W. DE A.; ALMEIDA, P. DE S.; VIANA, M. B.; ZAIAT, M.; LEITAO, R. C. Using fruit and vegetable waste to generate hydrogen through dark fermentation. Engenharia Sanitária e Ambiental, v. 29, e20240013, 2024. Uso de resíduos de frutas e verduras para gerar hidrogênio por meio da fermentação escura.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
|  |
| 7. | | LAGE, J. F.; PAULINO, P. V. R.; PEREIRA, L. G. R.; DUARTE, M. S.; VALADARES FILHO, S. C.; OLIVEIRA, A. S.; SOUZA, N. K. P.; LIMA, J. C. M. Carcass characteristics of feedlot lambs fed crude glycerin contaminated with high concentrations of crude fat. Meat Science, v. 96, n. 1, p. 108-113, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | WELLER, M. M.; ALBINO, R. L.; MARCONDES, M. I.; SILVA, W.; DANIELS, K. M.; CAMPOS, M. M.; DUARTE, M. S.; MESCOUTO, M. L.; SILVA, F. F.; GUIMARÃES, S. E. F. Effects of nutrient intake level on mammary parenchyma growth and gene expression in crossbred (Holstein × Gyr) prepubertal heifers. Journal of Dairy Science, v. 99, n. 12, p. 9962-9973, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | GIONBELLI, T. R. S.; VELOSO, C. M.; ROTTA, P. P.; VALADARES FILHO, S. C.; CARVALHO, B. C. de; M. I. MARCONDES; CUNHA, C. S.; NOVAES, M. A. S.; PREZOTTO, L. D.; DUARTE, M. S.; GIONBELLI, M. P. Foetal development of skeletal muscle in bovines as a function of maternal nutrition, foetal sex and gestational age. Journal of Animal Physiology and Animal Nutrition, v. 102, p. 545-556, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | GIONBELLI, T. R. S.; ROTTA, P. P.; VELOSO, C. M.; MARCONDES, M. I.; VALADARES FILHO, S. C.; CARVALHO, B. C. de; LOVATTI, J. V. R.; CUNHA, C. S.; NOVAIS, M. A. S.; DUARTE, M. S.; GIONBELLI, M. P. Investigation of effects of maternal nutrition intensification and fetal sex on development of skeletal muscle of bovine fetuses. In: ADSA ASAS JOINT ANNUAL MEETING, 2015, Orlando. Proceedings... Orlando: ADSA: ASAS, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | GONDIM, R. S.; CRISOSTOMO, L. A.; MAIA, A. de H. N.; SOUSA, J. A. de; BARROS, V. da S.; SILVA, A. R. da C.; GRANGEIRO, L. C.; GOMES FILHO, A. A. H.; DUARTE, M. S.; GONDIM, T. de A.; CORDEIRO, E. de A.; GURGEL, M. C.; MARQUES, A. A. A. Estoque de carbono no solo em diferentes sistemas de produção do meloeiro. Fortaleza: Embrapa Agroindústria Tropical, 2015. 32 p. (Embrapa Agroindústria Tropical. Boletim de Pesquisa e Desenvolvimento, 97).| Tipo: Boletim de Pesquisa e Desenvolvimento |
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| 12. | | GIONBELLI, T. R. S.; ROTTA, P. P.; VELOSO, C. M.; VALADARES FILHO, S. C.; CARVALHO, B. C. de; MARCONDES, M. I.; FERREIRA, M. F. L.; SOUZA, J. V. F.; SANTOS, J. S. A. A.; LACERDA, L. C.; DUARTE, M. S.; GIONBELLI, M. P. Intestinal development of bovine foetuses during gestation is affected by foetal sex and maternal nutrition. Journal of Animal Physiology and Animal Nutrition, v. 101, n. 3, p. 493-501, 2017. Publicado online em 16 ago. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 12 | |
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