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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com rosangela.lacerda@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
16/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUIMARAES, A. S.; DORNELES, E. M. S.; ANDRADE, G. I.; LAGE, A. P.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. |
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Afiliação: |
ALESSANDRO DE SA GUIMARAES, CNPGL; ELAINE MARIA SELES DORNELES, UFMG; GIOVANNA IVO ANDRADE, UFMG; ANDREY PEREIRA LAGE, UFMG; GEPOC; ANDERSON MIYOSHI, UFMG; VASCO AZEVEDO, UFMG; GEPOC; AURORA MARIA GUIMARÃES GOUVEIA, UFMG; GEPOC; MARCOS BRYAN HEINEMANN, UFMG; GEPOC. |
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Título: |
Molecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Veterinary Microbiology, v. 153, n. 3/4, p. 299-306, 2011. |
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DOI: |
https://doi.org/10.1016/j.vetmic.2011.06.002 |
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Idioma: |
Inglês |
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Conteúdo: |
Caseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates. MenosCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10... Mostrar Tudo |
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Palavras-Chave: |
ERIC-PCR; RAPD. |
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Thesagro: |
Corynebacterium Pseudotuberculosis. |
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Thesaurus Nal: |
caseous lymphadenitis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02507naa a2200265 a 4500
001 1910089
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.vetmic.2011.06.002$2DOI
100 1 $aGUIMARAES, A. S.
245 $aMolecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR.$h[electronic resource]
260 $c2011
520 $aCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates.
650 $acaseous lymphadenitis
650 $aCorynebacterium Pseudotuberculosis
653 $aERIC-PCR
653 $aRAPD
700 1 $aDORNELES, E. M. S.
700 1 $aANDRADE, G. I.
700 1 $aLAGE, A. P.
700 1 $aMIYOSHI, A.
700 1 $aAZEVEDO, V.
700 1 $aGOUVEIA, A. M. G.
700 1 $aHEINEMANN, M. B.
773 $tVeterinary Microbiology$gv. 153, n. 3/4, p. 299-306, 2011.
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Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 6 | |
| 1. |  | KOFFLER, S.; MENEZES, C.; MENEZES, P. R.; KLEINERT, A. de M. P.; IMPERATRIZ-FONSECA, V. L.; POPE, N.; JAFFÉ, R. Efeitos do clima e do manejo sobre a produção de mel pela jandaíra: o que podemos aprender com a meliponicultura de Mossoró, Rio Grande do Norte? In: IMPERATRIZ-FONSECA, V. L.; KOEDAM, D.; HRNCIR, M. (Ed.). A abelha jandaíra no passado, no presente e no futuro. Mossoró, RN: EDUFERSA, 2017. Cap. 17, p. 175-182.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 2. | | KOFFLER, S.; MENEZES, C.; MENEZES, P. R.; KLEINERT, A. de M. P.; IMPERATRIZ-FONSECA, V. L.; POPE, N.; JAFFE, R. Temporal Variation in Honey Production by the Stingless Bee Melipona subnitida (Hymenoptera: Apidae): Long-Term Management Reveals its Potential as a Commercial Species in Northeastern Brazil. Journal of Economic Entomology, v. 108, n. 3, p. 858-867, June 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 3. | | KOFFLER, S.; MENEZES, C.; MENEZES, P.; KLEINERT, A. de M. P.; IMPERATRIZ-FONSECA, V. L.; POPE, N.; JAFFÉ, R. Stingless beekeeping development in Northeastern Brazil may be impaired my extreme weather conditions. In: ENCONTRO SOBRE ABELHAS, 11., 2015, Ribeirão Preto. Anais... Ribeirão Preto: USP: FCLRP, 2015. p. 107.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 4. | | CAMPBELL, A. J.; CARVALHEIRO, L. G.; MAUES, M. M.; JAFFÉ, R.; GIANNINI, T. C.; FREITAS, M. A. B.; COELHO, B. W. T.; MENEZES, C. Anthropogenic disturbance of tropical forests threatens pollination services to açaí palm in the Amazon river delta. Journal of Applied Ecology, v. 55, n. 4, p. 1725-1736, July 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 5. | | JAFFÉ, R.; POPE, N.; CARVALHO, A. T.; MAIA, U. M.; BLOCHTEIN, B.; CARVALHO, C. A. L. de; CARVALHO-ZILSE, G. A.; FREITAS, B. M.; MENEZES, C.; RIBEIRO, M. de F.; VENTURIERI, G. C.; IMPERATRIZ-FONSECA, V. L. Abelhas para o desenvolvimento: Pesquisa Brasileira procura transformar a criação de abelhas nativas numa ferramenta de desenvolvimento sustentável. Mensagem Doce, n. 132, jul. 2015.| Tipo: Artigo de Divulgação na Mídia | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Semiárido. |
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| 6. | | JAFFÉ, R.; POPE, N.; CARVALHO, A. T.; MAIA, U. M.; BLOCHTEIN, B.; CARVALHO, C. A. L. de; CARVALHO-ZILSE, G. A.; FREITAS, B. M.; MENEZES, C.; RIBEIRO, M. de F.; VENTURIERI, G. C.; IMPERATRIZ-FONSECA, V. L. Bees for development: brazilian survey reveals how to optimize stingless beekeeping. Plos One, v. 10, n. 3, e0121157, Mar. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Semiárido. |
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| Registros recuperados : 6 | |
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