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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/04/2011 |
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Data da última atualização: |
29/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GOMES, L. M. C.; CASTRO, A. V.; DIAS, L. O.; GOMES, F. P.; ALMEIDA, A-A. F.; PIROVANI, C. P.; GESTEIRA, A. da S. |
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Afiliação: |
Luana Mahé Costa Gomes, UESC; A. V. CASTRO, UESC; L. O. DIAS, UESC; Fábio Pinto Gomes, UESC; Alex-Alan Furtado Almeida, UESC; Carlos Priminho Pirovani, UESC; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Cadmium-induced changes in leaf protein profile of Caesalpinia peltophoroides Benth (sibipiruna): protein extraction and bidimentional electrophoresis. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos... [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM. |
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Idioma: |
Português |
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Notas: |
pdf 34634 |
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Conteúdo: |
The specie C.peltophoroides is used as bioindicator of environmental pollution, being suitable for revegetation of degraded areas. Works related to cadmium (Cd) tolerance in this species are still being initiated. In this way, analysis of protein profile aims to assist in the identification of stress-induced protein expression. Seedlings growing in nutrient solution were submitted to two treatments: no Cd and high Cd concentration (32 mg L-1) in a completely randomized design. Leaves from the two treatments were collected at different times of exposure to Cd (0, 6, 12, 24, 48, 72 and 96 h) and fixed in liquid N2. All samples were freeze dried, macerated and washed with TCA/acetone, TCA/water and finally acetone. Proteins were quantified using 2-D Quanti kit (GE Healthcare) and analyzed by SDS-PAGE and 2D-PAGE. Preliminary analysis of proteins in SDS-PAGE showed several bands below 25 kDa, when compared with the control (no Cd), in differents exposure times. The two samples from different exposure times to Cd (24 and 72 h) were selected after analysis of SDS-PAGE and were used in two-dimensional electrophoresis. For isoelectric focusing, we used 380 ?g of protein sample diluted in rehydration buffer strips on linear 13 cm (pH 4-7) using equipment IPGphor 3. The second dimension was performed on polyacrylamide gels with 12.5% in the system Hoefer SE 600 Ruby ?. The gels were fixed with 10% acetic acid and 40% ethanol, stained with Coomassie Brilliant Blue and faded washes in Milli-Q water until the appearance of spots. The gel images were captured in 2D Scanner Magic and analyzed with the software ImageMaster 2D Platinum (Amersham Biosciences) and manual detection. In 2D-PAGE spots were visualized 99 spots (41 differentially expressed) in the 24 h and 122 spots (29 differentially expressed) in the 72 h, both with quality. Most intense spots were analyzed in the Expasy Tagident (www.expasy.ch/), based on its mass and isoelectric point. Taxonomic group and keywords were used to search in the protein database. Preliminary results identified proteins such as: aquaporins, heat shock (HSP), cold and drought-regulated protein (CORA), photosystem I assembly, NAD(P)H-quinone oxidoreductase subunit and cytochrome b6-f complex subunit in Populus, Arabidopsis and Pisum sativum. Some spots were not found in the database. Spots will be selected for the protein sequencing by mass spectrometry. This is a pioneering study involving proteomic data in order to support the hypothesis that C. peltophoroides can be considered a promising species in tolerance to Cd stress. MenosThe specie C.peltophoroides is used as bioindicator of environmental pollution, being suitable for revegetation of degraded areas. Works related to cadmium (Cd) tolerance in this species are still being initiated. In this way, analysis of protein profile aims to assist in the identification of stress-induced protein expression. Seedlings growing in nutrient solution were submitted to two treatments: no Cd and high Cd concentration (32 mg L-1) in a completely randomized design. Leaves from the two treatments were collected at different times of exposure to Cd (0, 6, 12, 24, 48, 72 and 96 h) and fixed in liquid N2. All samples were freeze dried, macerated and washed with TCA/acetone, TCA/water and finally acetone. Proteins were quantified using 2-D Quanti kit (GE Healthcare) and analyzed by SDS-PAGE and 2D-PAGE. Preliminary analysis of proteins in SDS-PAGE showed several bands below 25 kDa, when compared with the control (no Cd), in differents exposure times. The two samples from different exposure times to Cd (24 and 72 h) were selected after analysis of SDS-PAGE and were used in two-dimensional electrophoresis. For isoelectric focusing, we used 380 ?g of protein sample diluted in rehydration buffer strips on linear 13 cm (pH 4-7) using equipment IPGphor 3. The second dimension was performed on polyacrylamide gels with 12.5% in the system Hoefer SE 600 Ruby ?. The gels were fixed with 10% acetic acid and 40% ethanol, stained with Coomassie Brilliant Blue and faded washes in M... Mostrar Tudo |
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Palavras-Chave: |
Environmental pollution; Proteomic. |
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Thesaurus Nal: |
abiotic stress; heavy metals; phytoremediation. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03476nam a2200253 a 4500
001 1886916
005 2011-04-29
008 2011 bl uuuu u00u1 u #d
100 1 $aGOMES, L. M. C.
245 $aCadmium-induced changes in leaf protein profile of Caesalpinia peltophoroides Benth (sibipiruna)$bprotein extraction and bidimentional electrophoresis.
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos... [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.$c2011
500 $apdf 34634
520 $aThe specie C.peltophoroides is used as bioindicator of environmental pollution, being suitable for revegetation of degraded areas. Works related to cadmium (Cd) tolerance in this species are still being initiated. In this way, analysis of protein profile aims to assist in the identification of stress-induced protein expression. Seedlings growing in nutrient solution were submitted to two treatments: no Cd and high Cd concentration (32 mg L-1) in a completely randomized design. Leaves from the two treatments were collected at different times of exposure to Cd (0, 6, 12, 24, 48, 72 and 96 h) and fixed in liquid N2. All samples were freeze dried, macerated and washed with TCA/acetone, TCA/water and finally acetone. Proteins were quantified using 2-D Quanti kit (GE Healthcare) and analyzed by SDS-PAGE and 2D-PAGE. Preliminary analysis of proteins in SDS-PAGE showed several bands below 25 kDa, when compared with the control (no Cd), in differents exposure times. The two samples from different exposure times to Cd (24 and 72 h) were selected after analysis of SDS-PAGE and were used in two-dimensional electrophoresis. For isoelectric focusing, we used 380 ?g of protein sample diluted in rehydration buffer strips on linear 13 cm (pH 4-7) using equipment IPGphor 3. The second dimension was performed on polyacrylamide gels with 12.5% in the system Hoefer SE 600 Ruby ?. The gels were fixed with 10% acetic acid and 40% ethanol, stained with Coomassie Brilliant Blue and faded washes in Milli-Q water until the appearance of spots. The gel images were captured in 2D Scanner Magic and analyzed with the software ImageMaster 2D Platinum (Amersham Biosciences) and manual detection. In 2D-PAGE spots were visualized 99 spots (41 differentially expressed) in the 24 h and 122 spots (29 differentially expressed) in the 72 h, both with quality. Most intense spots were analyzed in the Expasy Tagident (www.expasy.ch/), based on its mass and isoelectric point. Taxonomic group and keywords were used to search in the protein database. Preliminary results identified proteins such as: aquaporins, heat shock (HSP), cold and drought-regulated protein (CORA), photosystem I assembly, NAD(P)H-quinone oxidoreductase subunit and cytochrome b6-f complex subunit in Populus, Arabidopsis and Pisum sativum. Some spots were not found in the database. Spots will be selected for the protein sequencing by mass spectrometry. This is a pioneering study involving proteomic data in order to support the hypothesis that C. peltophoroides can be considered a promising species in tolerance to Cd stress.
650 $aabiotic stress
650 $aheavy metals
650 $aphytoremediation
653 $aEnvironmental pollution
653 $aProteomic
700 1 $aCASTRO, A. V.
700 1 $aDIAS, L. O.
700 1 $aGOMES, F. P.
700 1 $aALMEIDA, A-A. F.
700 1 $aPIROVANI, C. P.
700 1 $aGESTEIRA, A. da S.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 20 | |
| 5. |  | SENA, L. de; SANTANA, T. S. de; JESUS NETA, P. de; JESUS, J. L. de; VIANA, E. de S.; REIS, R. C.; EVANGELISTA-BARRETO, N. S. Efeito de diferentes métodos de conservação na estabilidade da cor e na qualidade microbiológica de biomassa de banana verde. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 13., 2019. Foco e valor : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura,2019. 119 p. il.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | NASCIMENTO, A. S. do; JESUS NETA, P. de; OLIVEIRA, L. A. de; JESUS, J. L. de; SILVA, M. P.; SANTOS, V. da S. Determinação do teor de compostos cianogênicos e carotenoides totais em híbridos de mandioca de mesa. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 13., 2019. Foco e valor : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura,2019.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | OLIVEIRA, L. A. de; SANTOS, V. da S.; RANGEL, M. A. S.; JESUS NETA, P. de; RINGENBERG, R.; JESUS, J. L. de; LEDO, C. A. da S. Desempenho de genótipos de mandioca promissores para a indústria e caracterização do amido. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2024. 13 p. il. (Embrapa Mandioca e Fruticultura. Boletim de Pesquisa e Desenvolvimento, 158). ODS.| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 8. | | RODRIGUES, L. A.; FERNANDES, J. C.; ALMEIDA, J. M.; JESUS NETA, P. de; VIANA, E. de S.; REIS, R. C.; SASAKI, F. F. C.; JUNGHANS, D. T. Análise sensorial de abacaxis 'Pérola' e 'BAG 344', produzidos sob quatro sistemas de cultivo, no semiárido da Bahia. In : JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 12., 2018. Ciência profissional : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2019. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 10. | | REIS, R. C.; VIANA, E. de S.; ALMEIDA, K. DE O.; SILVA, V. DA; SANTOS, M. F. F.; JESUS NETA, P. DE; TRINDADE, M. R. DE S. Características nutricionais e sensoriais de frutos de variedades de mamoeiro cultivadas em um sistema orgânico de produção. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2025. 11 p. (Embrapa Mandioca e Fruticultura. Boletim de Pesquisa e Desenvolvimento, 159).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | JESUS NETA, P. de; OLIVEIRA, L. A. de; JESUS, J. L. de; RINGENBERG, R.; RANGEL, M. A. S.; LEONEL, M.; FERNANDES, A. M.; SANTOS, V. da S. Caracterização do teor de amilose, pH e acidez do amido extraído de clones de mandioca em diferentes idades. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 14., 2020. Ciência em tempos de crise: resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2020. 112 p. il.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 12. | | JESUS, J. L. de; LEONEL, M.; VIANA, E. de S.; AMORIM, E. P.; REIS, R. C.; CARVALHO, C. W. P. de; JESUS NETA, P. DE; LEONEL, S. Banana and Plantain Starches: exploring differences and potential applications. Horticulturae, v. 11, p. 1214, 2025.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos; Embrapa Mandioca e Fruticultura. |
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| 13. | | ARAÚJO JÚNIOR, I. T.; VIANA, E. de S.; SILVEIRA, R. B.; JESUS NETA, P. de; ALMEIDA, L. S. de; REIS, R. C.; PADUA, T. R. P. de. Qualidade físico-química e estabilidade dos compostos bioativos na polpa congelada de frutos de mamoeiro cultivados em sistema orgânico. In: CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 15., 2021. Mulheres na ciência desafios, oportunidades e conquistas. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2021. 109 f. il. PDF.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | SILVEIRA, R. B.; VIANA, E. de S.; JESUS NETA, P. de; ARAÚJO JÚ-NIOR, I. T.; ALMEIDA, L. S. de; REIS, R. C.; JESUS, O. N. de. Qualidade de frutos de novos híbridos de maracujazeiro cultivados em sistema orgânico. In: CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 15., 2021. Mulheres na ciência desafios, oportunidades e conquistas. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2021. 109 f. il. PDF.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 15. | | ASSIS, S. L. F. de; MACHADO, L. da S. C.; REIS, R. C.; VIANA, E. de S.; JESUS NETA, P. de. Quantificação de amido total e resistente em genótipos de bananas e plátanos. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 10., 2016: Cruz das Almas, BA. Traduzindo ciência para o mundo : resumos. Brasília, DF : Embrapa, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 18. | | ALMEIDA, L. S. de; REIS, R. C.; VIANA, E. de S.; ARAUJO JÚNIOR, I. T.; CAVALCANTE, M, C, B.; JESUS NETA, P. de. Vitamina C e carotenoides em frutos de maracujá de casca roxa cultivados no sistema orgânico de produção. In: CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 15., 2021. Mulheres na ciência desafios, oportunidades e conquistas. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2021. 109 f. il. PDF.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 19. | | ALMEIDA, P. A. M.; OLIVEIRA, L. A. de; JESUS NETA, P. de; JESUS, J. L. de; RANGEL, M. A. S.; RINGENBERG, R.; LEONEL, M.; FERNANDES, A. M.; SANTOS, V. da S. Perfil de viscosidade e teor de fósforo do amido extraído de diferentes clones de mandioca em diferentes idades. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 14., 2020. Ciência em tempos de crise: resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2020. 112 p. il.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 20. | | NASCIMENTO, A. S. do; JESUS NETA, P. de; RODRIGUES, L. A.; OLIVEIRA, L. A. de; JESUS, J. L. de; SANTOS, V. da S.; ALMEIDA, L. H. S.; RIBEIRO, O. L. Quantificação de compostos cianogênicos nas folhas de diferentes variedades mandioca. In : JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 12., 2018. Ciência profissional : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2019. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 20 | |
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