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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Ocidental. Para informações adicionais entre em contato com cpaa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
30/03/2011 |
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Data da última atualização: |
02/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHEN, S. X.; BOGERD, J.; ANDERSSON, E.; ALMEIDA, F. F. L. de; TARANGER, G. L.; SCHULZ, R. W. |
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Afiliação: |
SHI X CHEN, UTRECHT UNIVERSITY; JAN BOGERD, UTRECHT UNIVERSITY; EVA ANDERSSON, INSTITUTE OF MARINE RESEARCH; FERNANDA FERREIRA LOUREIRO DE ALMEIDA, CPAA; GEIR LASSE TARANGER, INSTITUTE OF MARINE RESEARCH; RUDIGER W. SCHULZ, UTRECHT UNIVERSITY. |
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Título: |
Cloning, pharmacological characterization, and expression analysis of Atlantic salmon (Salmo salar L.) nuclear progesterone receptor. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Reproduction, v. 141, p. 491-500, Apr. 2011. |
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DOI: |
10.1530/REP-10-0224 |
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Idioma: |
Inglês |
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Conteúdo: |
To better understand the role(s) of progestogens during early stages of spermatogenesis, we carried out studies on the nuclear progesterone receptor (Pgr) of the Atlantic salmon. Its open-reading frame shows the highest similarity with other piscine Pgr proteins. When expressed in mammalian cells, salmon Pgr exhibited progestogen-specific, dose-dependent induction of reporter gene expression, with 17?,20?-dihydroxy-4-pregnen-3-one (DHP) showing the highest potency. We then analyzed testicular pgr mRNA and DHP plasma levels in animals during the onset of spermatogenesis, which were exposed to natural light or to constant light, to induce significant differences in testis growth. Grouping of the animals according to their progress through spermatogenesis showed that testicular pgr mRNA levels as well as DHP plasma levels first increased when germ cells had reached the stage of late type B spermatogonia and further increased when entered meiosis, i.e. when spermatocytes were present. However, in situ hybridization studies revealed that pgr mRNA expression was restricted to Sertoli cells, with a strong signal in Sertoli cells contacting type A/early type B spermatogonia, while Sertoli cells contacting larger germ cell clones with further differentiated stages (e.g. late type B spermatogonia) were less intensely/not stained. We conclude that the increase in pgr mRNA levels per pair of testis reflects, at least in part, the increased number of Sertoli cells enveloping type A and early type B spermatogonia. We propose that Sertoli cell-expressed Pgr may mediate DHP-stimulated early steps in spermatogenesis in Atlantic salmon, such as an increase in the number of new spermatogonial cysts. MenosTo better understand the role(s) of progestogens during early stages of spermatogenesis, we carried out studies on the nuclear progesterone receptor (Pgr) of the Atlantic salmon. Its open-reading frame shows the highest similarity with other piscine Pgr proteins. When expressed in mammalian cells, salmon Pgr exhibited progestogen-specific, dose-dependent induction of reporter gene expression, with 17?,20?-dihydroxy-4-pregnen-3-one (DHP) showing the highest potency. We then analyzed testicular pgr mRNA and DHP plasma levels in animals during the onset of spermatogenesis, which were exposed to natural light or to constant light, to induce significant differences in testis growth. Grouping of the animals according to their progress through spermatogenesis showed that testicular pgr mRNA levels as well as DHP plasma levels first increased when germ cells had reached the stage of late type B spermatogonia and further increased when entered meiosis, i.e. when spermatocytes were present. However, in situ hybridization studies revealed that pgr mRNA expression was restricted to Sertoli cells, with a strong signal in Sertoli cells contacting type A/early type B spermatogonia, while Sertoli cells contacting larger germ cell clones with further differentiated stages (e.g. late type B spermatogonia) were less intensely/not stained. We conclude that the increase in pgr mRNA levels per pair of testis reflects, at least in part, the increased number of Sertoli cells enveloping type A and e... Mostrar Tudo |
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Thesagro: |
Reprodução. |
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Categoria do assunto: |
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Marc: |
LEADER 02334naa a2200205 a 4500
001 1884099
005 2018-02-02
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1530/REP-10-0224$2DOI
100 1 $aCHEN, S. X.
245 $aCloning, pharmacological characterization, and expression analysis of Atlantic salmon (Salmo salar L.) nuclear progesterone receptor.
260 $c2011
520 $aTo better understand the role(s) of progestogens during early stages of spermatogenesis, we carried out studies on the nuclear progesterone receptor (Pgr) of the Atlantic salmon. Its open-reading frame shows the highest similarity with other piscine Pgr proteins. When expressed in mammalian cells, salmon Pgr exhibited progestogen-specific, dose-dependent induction of reporter gene expression, with 17?,20?-dihydroxy-4-pregnen-3-one (DHP) showing the highest potency. We then analyzed testicular pgr mRNA and DHP plasma levels in animals during the onset of spermatogenesis, which were exposed to natural light or to constant light, to induce significant differences in testis growth. Grouping of the animals according to their progress through spermatogenesis showed that testicular pgr mRNA levels as well as DHP plasma levels first increased when germ cells had reached the stage of late type B spermatogonia and further increased when entered meiosis, i.e. when spermatocytes were present. However, in situ hybridization studies revealed that pgr mRNA expression was restricted to Sertoli cells, with a strong signal in Sertoli cells contacting type A/early type B spermatogonia, while Sertoli cells contacting larger germ cell clones with further differentiated stages (e.g. late type B spermatogonia) were less intensely/not stained. We conclude that the increase in pgr mRNA levels per pair of testis reflects, at least in part, the increased number of Sertoli cells enveloping type A and early type B spermatogonia. We propose that Sertoli cell-expressed Pgr may mediate DHP-stimulated early steps in spermatogenesis in Atlantic salmon, such as an increase in the number of new spermatogonial cysts.
650 $aReprodução
700 1 $aBOGERD, J.
700 1 $aANDERSSON, E.
700 1 $aALMEIDA, F. F. L. de
700 1 $aTARANGER, G. L.
700 1 $aSCHULZ, R. W.
773 $tReproduction$gv. 141, p. 491-500, Apr. 2011.
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Embrapa Amazônia Ocidental (CPAA) |
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| Registros recuperados : 7 | |
| 1. |  | ABREU, C. S. de; CAMPOS, M. da S.; OLIVEIRA, R. S. de; MARINS, M. S.; GOMES, E. A.; STOIANOFF, M. A. de R.; OLIVEIRA-PAIVA, C. A. Efeito da adubação fosfatada sobre a população de micro-organismos solubilizadores e mineralizadores de fósforo isolados em rizosfera de milho. In: CONGRESSO NACIONAL DE MILHO E SORGO, 31., 2016, Bento Gonçalves. Milho e sorgo: inovações, mercados e segurança alimentar: anais. Sete Lagoas: Associação Brasileira de Milho e Sorgo, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 2. | | BONIEK, D.; MENDES, I. de C.; OLIVEIRA-PAIVA, C. A.; LANA, U. G. de P.; SANTOS, A. F. B. dos; STOIANOFF, M. A. de R. Ecology and identification of environmental fungi and metabolic processes involved in the biodeterioration of Brazilian soapstone historical monuments. Letters in Applied Microbiology, Oxford, v. 65, p. 431-438, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 3. | | CAMPOS, J. da S.; VITAL JÚNIOR, A. C.; BONIEK, D.; SILVA, D. L. da; LANA, U. G. de P.; FERNANDES, J. V.; STOIANOFF, M. A. de R.; ANDRADE, V. S. Identification and evaluation of thermotolerance of yeasts from milk in natura exposed to high temperature and slow and fast pasteurization. Brazilian Journal of Microbiology, v. 54, p. 1075-1082, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 4. | | ABREU, C. S. de; MARINS, M. S.; OLIVEIRA, R. S. de; CAMPOS, M. da S.; OLIVEIRA-PAIVA, C. A.; GOMES, E. A.; SANTOS, V. L. dos; MARRIEL, I. E.; STOIANOFF, M. A. de R. Atividade enzimática de fosfatases em solo cultivado com milho inoculado com micro-organismos solubilizadores de fosfato e adubado com fontes de fósforo de diferente solubilidade. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. p. 1025. FertBio 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 5. | | CONCEIÇÃO, R. R. P. da; QUEIROZ, V. A. V.; SIMEONE, M. L. F.; SILVA, D. D. da; CARMO, P. H. F. do; MENEZES, C. B. de; FIGUEIREDO, J. E. F.; STOIANOFF, M. A. de R. Does sorghum phenolic extract have antifungal effect? Brazilian Journal of Microbiology, v. 55, p. 1829-1839, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 6. | | ABREU, C. S. de; OLIVEIRA-PAIVA, C. A.; FIGUEIREDO, J. E. F.; TAVARES, A. N. G.; GOMES, E. A.; SANTOS, V. L. dos; MARRIEL, I. E.; STOIANOFF, M. A. de R. Seleção e caracterização de bactérias endofíticas de milho com potencial para biossolubilização de fosfato. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. p. 1004. FertBio 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 7. | | BONIEK, D.; OLIVEIRA, L. V. N. de; RIBEIRO, N. de Q.; OLIVEIRA-PAIVA, C. A.; LANA, U. G. de P.; SANTOS, A. F. B. dos; STOIANOFF, M. A. de R. Effect of environmental factors on rock-inhabiting fungal communities from brazilian soapstone samples. Journal of Environmental Science and Engineering B, v. 6, p. 55-71, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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