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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/08/2010 |
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Data da última atualização: |
22/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, E. H.; SOARES, T. L.; SOUZA, F. V. D.; SANTOS-SEREJO, J. A. dos. |
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Afiliação: |
Everton Hilo Souza, UFRB; Tailane Leila Soares, UFRB; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF. |
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Título: |
Micropropagation of Heliconia rostrata and Heliconia bihai from mature zygotic embryos. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Acta Horticulturae, Leuven, n. 865, p.315-320, 2010. |
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ISSN: |
0567-7572 |
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Idioma: |
Inglês |
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Conteúdo: |
The difficulty in the micropropagation of many species of Heliconaceas has been mainly due to the high frequency of endogenous contamination. Embryo culture techniques have been used to generate normal seedlings to be used as initial explants. This work aimed to develop a plant production protocol using zygotic embryos from Heliconia rostrata and H. bihai. Embryos from mature fruits were disinfested, excised and cultivated in MS medium solidified with 8% of agar, 6% of sucrose (MS + 6% S) and pH 5,8, autoclaved at 120°C for 20 minutes. The treatments were: T01: Control (MS + 6% S); T02: (MS + 6% S) + 0,25% activated charcoal; T03: (MS + 6% S) + 1,0 mg L-1 BAP; T04: (MS + 6% S) + 1,0 mg L-1 BAP + 0,25% activated charcoal; T05: (MS + 6% S) + 1,0 mg L-1 BAP + 1,0 mg L-1 GA3; T06: (MS + 6% S) + 1,0 mg L-1 BAP + 1 mg L-1 GA3 + 0,25% of activated charcoal. The embryos were maintained in dark conditions for 45 days with temperature of 27±1°C. Treatment T02 promoted the best percentage of germination (100%) for both species and T05 the lower results with 20% and 35% of germination for H. rostrata and H. bihai respective at 30 days of culture. Callus formation was observed in T05 and T06. The activated charcoal was essential to embryo germination. These seedlings were used as explants in the micropropagation assays in MS medium supplemented with these treatments: T01: MS; T02: MS + 0,25% of activated charcoal; T03: MS + 4,0 mg L-1 de BAP; T04: MS + 4,0 mg L-1 BAP + 0,25% of activated charcoal. For the multiplication stage the best medium was the control treatment with no growth regulators and no activated charcoal. A bacterial contamination appeared during subsequence subculture making the micropropagation of these species unfeasible. MenosThe difficulty in the micropropagation of many species of Heliconaceas has been mainly due to the high frequency of endogenous contamination. Embryo culture techniques have been used to generate normal seedlings to be used as initial explants. This work aimed to develop a plant production protocol using zygotic embryos from Heliconia rostrata and H. bihai. Embryos from mature fruits were disinfested, excised and cultivated in MS medium solidified with 8% of agar, 6% of sucrose (MS + 6% S) and pH 5,8, autoclaved at 120°C for 20 minutes. The treatments were: T01: Control (MS + 6% S); T02: (MS + 6% S) + 0,25% activated charcoal; T03: (MS + 6% S) + 1,0 mg L-1 BAP; T04: (MS + 6% S) + 1,0 mg L-1 BAP + 0,25% activated charcoal; T05: (MS + 6% S) + 1,0 mg L-1 BAP + 1,0 mg L-1 GA3; T06: (MS + 6% S) + 1,0 mg L-1 BAP + 1 mg L-1 GA3 + 0,25% of activated charcoal. The embryos were maintained in dark conditions for 45 days with temperature of 27±1°C. Treatment T02 promoted the best percentage of germination (100%) for both species and T05 the lower results with 20% and 35% of germination for H. rostrata and H. bihai respective at 30 days of culture. Callus formation was observed in T05 and T06. The activated charcoal was essential to embryo germination. These seedlings were used as explants in the micropropagation assays in MS medium supplemented with these treatments: T01: MS; T02: MS + 0,25% of activated charcoal; T03: MS + 4,0 mg L-1 de BAP; T04: MS + 4,0 mg L-1 BAP + 0,25% of activated... Mostrar Tudo |
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Palavras-Chave: |
Heliconia rostrata; Zygotic embryos. |
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Thesagro: |
Floricultura; Planta Ornamental. |
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Thesaurus Nal: |
Heliconia bihai; micropropagation. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02467naa a2200241 a 4500
001 1859859
005 2022-08-22
008 2010 bl uuuu u00u1 u #d
022 $a0567-7572
100 1 $aSOUZA, E. H.
245 $aMicropropagation of Heliconia rostrata and Heliconia bihai from mature zygotic embryos.$h[electronic resource]
260 $c2010
520 $aThe difficulty in the micropropagation of many species of Heliconaceas has been mainly due to the high frequency of endogenous contamination. Embryo culture techniques have been used to generate normal seedlings to be used as initial explants. This work aimed to develop a plant production protocol using zygotic embryos from Heliconia rostrata and H. bihai. Embryos from mature fruits were disinfested, excised and cultivated in MS medium solidified with 8% of agar, 6% of sucrose (MS + 6% S) and pH 5,8, autoclaved at 120°C for 20 minutes. The treatments were: T01: Control (MS + 6% S); T02: (MS + 6% S) + 0,25% activated charcoal; T03: (MS + 6% S) + 1,0 mg L-1 BAP; T04: (MS + 6% S) + 1,0 mg L-1 BAP + 0,25% activated charcoal; T05: (MS + 6% S) + 1,0 mg L-1 BAP + 1,0 mg L-1 GA3; T06: (MS + 6% S) + 1,0 mg L-1 BAP + 1 mg L-1 GA3 + 0,25% of activated charcoal. The embryos were maintained in dark conditions for 45 days with temperature of 27±1°C. Treatment T02 promoted the best percentage of germination (100%) for both species and T05 the lower results with 20% and 35% of germination for H. rostrata and H. bihai respective at 30 days of culture. Callus formation was observed in T05 and T06. The activated charcoal was essential to embryo germination. These seedlings were used as explants in the micropropagation assays in MS medium supplemented with these treatments: T01: MS; T02: MS + 0,25% of activated charcoal; T03: MS + 4,0 mg L-1 de BAP; T04: MS + 4,0 mg L-1 BAP + 0,25% of activated charcoal. For the multiplication stage the best medium was the control treatment with no growth regulators and no activated charcoal. A bacterial contamination appeared during subsequence subculture making the micropropagation of these species unfeasible.
650 $aHeliconia bihai
650 $amicropropagation
650 $aFloricultura
650 $aPlanta Ornamental
653 $aHeliconia rostrata
653 $aZygotic embryos
700 1 $aSOARES, T. L.
700 1 $aSOUZA, F. V. D.
700 1 $aSANTOS-SEREJO, J. A. dos
773 $tActa Horticulturae, Leuven$gn. 865, p.315-320, 2010.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 7 | |
| 1. |  | CANEL, N. G.; BEVACQUA, R. J.; HIRIART, M. I.; RABELO, N. C.; CAMARGO, L. S. de A.; SALAMONE, D. F. Approaches to improve intracytoplasmic sperm injection mediated transgenesis and maximize the use of sex-sorted sperm in bovine. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 41., 2015, Versailles. Reproductive performance: at the crossroads of genetics and the environment: abstracts. Versailles: IETS, 2015. p. 248.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | HIRIART, M. I.; CANEL, N. G.; GAMBINI, A.; BEVACQUA, R. J.; CAMARGO, L. S. de A.; SALAMONE, D. Ooplasmic transfer on the development of zona-free IVF bovine embryos. Animal Reproduction, v. 10, n. 3, p. 586, Jul./Sept. 2013. Suplemento. Edição dos abstracts do 27º Annual Meeting of the Brazilian Embryo Technology Society, 2013, Praia do Forte, BA.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | ROSA, I. La; CAMARGO, L. S. de A.; PEREIRA, M. M.; FERNANDEZ-MARTIN, R.; PAZ, D. A.; SALAMONE, D. F. Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos. Reproductive Biology and Endocrinology, v. 9, article 18, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | LA ROSA, I.; PEREIRA, M. M.; FERNANDEZ Y MARTIN, R.; CAMARGO, L. S. de A.; SALAMONE, D. Quantificação de transcritos em oócitos bovinos expostos ao BMP4 durante a maturação in vitro. Acta Scientiae Veterinariae, v. 38, p. 394, 2010. Edição dos resumos da 24a Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Porto de Galinhas, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | FREITAS, V. J. F. F.; MELO, L. M.; TEIXEIRA, D. I. A.; RADIS-BAPTISTA, G.; CAMARGO, L. S. de A.; SALAMONE, D. F. A espécie caprina como modelo para produção de proteínas recombinantes. Revista Brasileira de Reprodução Animal, v. 41, n. 1, p. 13-18, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | CANEL, N. G.; BEVACQUA, R. J.; HIRIART, M. I.; RABELO, N. C.; CAMARGO, L. S. de A.; ROMANATO, M.; CALVO, L. P.; SALAMONE, D. F. Sperm pretreatment with heparin and L-glutathione, sex-sorting, and double cryopreservation to improve intracytoplasmic sperm injection in bovine. Theriogenology, v. 93, p. 62-70, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | CAMPELO, I. S.; PEREIRA, A. F.; ALCÂNTARA-NETO, A. S.; CANEL, N. G.; SOUZA-FABJAN, J. M. G.; TEIXEIRA, D. I. A.; CAMARGO, L. S. de A.; MELO, L. M.; RÁDIS-BAPTISTA, G.; SALAMONE, D. F.; FREITAS, V. J. F. Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos. Zygote, v. 24, 1, p. 48-57, 2014. 10 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 7 | |
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