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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
10/01/2010 |
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Data da última atualização: |
21/11/2011 |
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Autoria: |
DWEIKAT, I.; MACKENZIE, S.; LEVY, N.; OHM, H. |
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Título: |
Pedigree assessment using RAPD-DGGE in cereal crop species. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Theoretical and Applied Genetics, v. 85, n. 5, p. 497-505, 1993. |
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Idioma: |
Inglês |
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Conteúdo: |
The introduction of molecular biology methodologies to plant improvement programs offers an invaluable opportunity for extensive germplasm characterization. However, the detection of adequate DNA polymorphism in self-pollinating species remains on obstacle. We have optimized a denaturing-gradient-gel electrophoresis (DGGE) system which, when used in combination with random amplified polymorphic DNA (RAPD) analysis, greatly facilitates the detection of reproducible DNA polymorphism among closely related plant lines. We have used this approach to estimate pedigree relationships among a spectrum of plant materials in wheat, barley and oat. Based on analysis with one or two primers, we were able to distinguish soft from hard winter wheat, and 2-rowed from 6-rowed barley. Further analysis with additional primers allowed resolution of polymorpisms even among closely related lines in highly selected populations. We placed 17 cultivars of oat into two distinct clusters that differed significantly from previous oat pedigree assessments. We believe that DGGE-RAPD is a superior method for detecting DNA polymorphism when compared to RFLP, agarose-RAPD, or polyacrylamide-RAPD methods. |
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Palavras-Chave: |
DNA polymorphism; Oat. |
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Thesaurus Nal: |
barley; DNA fingerprinting; wheat. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01747naa a2200217 a 4500
001 1839220
005 2011-11-21
008 1993 bl --- 0-- u #d
100 1 $aDWEIKAT, I.
245 $aPedigree assessment using RAPD-DGGE in cereal crop species.
260 $c1993
520 $aThe introduction of molecular biology methodologies to plant improvement programs offers an invaluable opportunity for extensive germplasm characterization. However, the detection of adequate DNA polymorphism in self-pollinating species remains on obstacle. We have optimized a denaturing-gradient-gel electrophoresis (DGGE) system which, when used in combination with random amplified polymorphic DNA (RAPD) analysis, greatly facilitates the detection of reproducible DNA polymorphism among closely related plant lines. We have used this approach to estimate pedigree relationships among a spectrum of plant materials in wheat, barley and oat. Based on analysis with one or two primers, we were able to distinguish soft from hard winter wheat, and 2-rowed from 6-rowed barley. Further analysis with additional primers allowed resolution of polymorpisms even among closely related lines in highly selected populations. We placed 17 cultivars of oat into two distinct clusters that differed significantly from previous oat pedigree assessments. We believe that DGGE-RAPD is a superior method for detecting DNA polymorphism when compared to RFLP, agarose-RAPD, or polyacrylamide-RAPD methods.
650 $abarley
650 $aDNA fingerprinting
650 $awheat
653 $aDNA polymorphism
653 $aOat
700 1 $aMACKENZIE, S.
700 1 $aLEVY, N.
700 1 $aOHM, H.
773 $tTheoretical and Applied Genetics$gv. 85, n. 5, p. 497-505, 1993.
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| 1. |  | BUENO, A. C. R.; DOMENICO, C. I.; FREITAS, R. A. de; JUSTINO, E. V.; NASCIMENTO, W. M. Envelhecimento acelerado e deterioração controlada para avaliação do vigor de sementes de lentilha. Horticultura Brasileira, Brasília, DF, v. 28, n. 2, p. S4262-S4268, jul. 2010. CD-ROM. Suplemento. Trabalho apresentado no Congresso Brasileiro de Olericultura, 50., 2010, Guarapari. Cinquenta anos contribuindo para a saúde da população brasileira. Trabalho A2754-T4555.| Tipo: Artigo em Anais de Congresso |
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