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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
15/06/2004 |
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Data da última atualização: |
25/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MEDEIROS, R. B.; RESENDE, R. de O.; AVILA, A. C. de. |
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Afiliação: |
RICARDO B. MEDEIROS, UNIVERSIDADE DE BRASÍLIA; RENATO DE O. RESENDE, UNIVERSIDADE DE BRASÍLIA; ANTONIO CARLOS DE AVILA, CNPH. |
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Título: |
The plant virus tomato spotted wilt tospovirus activates the immune system of its main insect vector, Frankliniella occidentalis. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Journal of Virology, v. 78, n. 10, p. 4976-4982, May 2004. |
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ISSN: |
1098-5514 |
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DOI: |
10.1128/JVI.78.10.4976–4982.2004 |
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Idioma: |
Inglês |
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Conteúdo: |
Tospoviruses have the ability to infect plants and their insect vectors. Tomato spotted wilt virus (TSWV), the type species in the Tospovirus genus, infects its most important insect vector, Frankliniella occidentalis, the western flower thrips (WFT). However, no detrimental effects on the life cycle or cytopathological changes have been reported in the WFT after TSWV infection, and relatively few viral particles can be observed even several days after infection. We hypothesized that TSWV infection triggers an immune response in the WFT. Using subtractive cDNA libraries to probe WFT DNA macroarrays, we found that the WFT’s immune system is activated by TSWV infection. The activated genes included (i) those encoding antimicrobial peptides, such as defensin and cecropin; (ii) genes involved in pathogen recognition, such as those encoding lectins; (iii) those encoding receptors that activate the innate immune response, such as Toll-3; and (iv) those encoding members of signal transduction pathways activated by Toll-like receptors, such as JNK kinase. Transcriptional upregulation of these genes after TSWV infection was confirmed by Northern analysis, and the kinetics of the immune response was measured over time. Several of the detected genes were activated at the same time that viral replication was first detected by reverse transcription-PCR. To our knowledge, this is the first report of the activation of an insect vector immune response by a plant virus. The results may lead to a better understanding of insects’ immune responses against viruses and may help in the future development of novel control strategies against plant viruses, as well as human and animal viruses transmitted by insect vectors. MenosTospoviruses have the ability to infect plants and their insect vectors. Tomato spotted wilt virus (TSWV), the type species in the Tospovirus genus, infects its most important insect vector, Frankliniella occidentalis, the western flower thrips (WFT). However, no detrimental effects on the life cycle or cytopathological changes have been reported in the WFT after TSWV infection, and relatively few viral particles can be observed even several days after infection. We hypothesized that TSWV infection triggers an immune response in the WFT. Using subtractive cDNA libraries to probe WFT DNA macroarrays, we found that the WFT’s immune system is activated by TSWV infection. The activated genes included (i) those encoding antimicrobial peptides, such as defensin and cecropin; (ii) genes involved in pathogen recognition, such as those encoding lectins; (iii) those encoding receptors that activate the innate immune response, such as Toll-3; and (iv) those encoding members of signal transduction pathways activated by Toll-like receptors, such as JNK kinase. Transcriptional upregulation of these genes after TSWV infection was confirmed by Northern analysis, and the kinetics of the immune response was measured over time. Several of the detected genes were activated at the same time that viral replication was first detected by reverse transcription-PCR. To our knowledge, this is the first report of the activation of an insect vector immune response by a plant virus. The results may lead ... Mostrar Tudo |
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Palavras-Chave: |
Interação vírus vetor; TSWV. |
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Thesagro: |
Inseto; Lycopersicon Esculentum; Praga; Tomate; Vetor; Vírus. |
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Thesaurus Nal: |
Frankliniella occidentalis; Tospovirus. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02599naa a2200289 a 4500
001 1776095
005 2024-01-25
008 2004 bl uuuu u00u1 u #d
022 $a1098-5514
024 7 $a10.1128/JVI.78.10.4976–4982.2004$2DOI
100 1 $aMEDEIROS, R. B.
245 $aThe plant virus tomato spotted wilt tospovirus activates the immune system of its main insect vector, Frankliniella occidentalis.$h[electronic resource]
260 $c2004
520 $aTospoviruses have the ability to infect plants and their insect vectors. Tomato spotted wilt virus (TSWV), the type species in the Tospovirus genus, infects its most important insect vector, Frankliniella occidentalis, the western flower thrips (WFT). However, no detrimental effects on the life cycle or cytopathological changes have been reported in the WFT after TSWV infection, and relatively few viral particles can be observed even several days after infection. We hypothesized that TSWV infection triggers an immune response in the WFT. Using subtractive cDNA libraries to probe WFT DNA macroarrays, we found that the WFT’s immune system is activated by TSWV infection. The activated genes included (i) those encoding antimicrobial peptides, such as defensin and cecropin; (ii) genes involved in pathogen recognition, such as those encoding lectins; (iii) those encoding receptors that activate the innate immune response, such as Toll-3; and (iv) those encoding members of signal transduction pathways activated by Toll-like receptors, such as JNK kinase. Transcriptional upregulation of these genes after TSWV infection was confirmed by Northern analysis, and the kinetics of the immune response was measured over time. Several of the detected genes were activated at the same time that viral replication was first detected by reverse transcription-PCR. To our knowledge, this is the first report of the activation of an insect vector immune response by a plant virus. The results may lead to a better understanding of insects’ immune responses against viruses and may help in the future development of novel control strategies against plant viruses, as well as human and animal viruses transmitted by insect vectors.
650 $aFrankliniella occidentalis
650 $aTospovirus
650 $aInseto
650 $aLycopersicon Esculentum
650 $aPraga
650 $aTomate
650 $aVetor
650 $aVírus
653 $aInteração vírus vetor
653 $aTSWV
700 1 $aRESENDE, R. de O.
700 1 $aAVILA, A. C. de
773 $tJournal of Virology$gv. 78, n. 10, p. 4976-4982, May 2004.
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Embrapa Hortaliças (CNPH) |
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| Registros recuperados : 9 | |
| 1. |  | RIBEIRO, M. E. R.; KOLLING, G. J.; ZANELA, M. B.; STUMPF, M. T.; SCHRAMM, R. C. Monitoramento da mastite em um rebanho jersey no sul do Rio Grande do Sul: resultados parciais 2011. In: FÓRUM DE PRODUÇÃO PECUÁRIA-LEITE, 14.; SALÃO DE TRABALHOS CIENTÍFICOS, 1., 2011, Cruz Alta. Anais... Cruz Alta: Unicruz Campus, 2011. p. 201-206.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 3. | | SAUGO, M.; JESUS, R, D, de; WEISSHEIMER, C. F.; RIBEIRO, M. E. R.; SCHRAMM, R. C.; ZANELA, M. B. Mastite causada por staphylococcus aureus: relato de caso. In: SEMANA INTEGRADA UFPEL, 5.; CONGRESSO DE INICIAÇÃO CIENTÍFICA, 28., 2019, Pelotas. [Anais]. 4 p. ENPOS| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 4. | | BARBOSA, R. S.; ZANELA, M. B.; PRATES, D. da F.; OLIVEIRA, F. M.; DALBANN, R. D.; WEISSHEIMER, C. F.; AZAMBUJA, A. A. de; SCHRAMM, R. C. Agentes etiológicos de mastite subclínica em vacas jersey de um rebanho no sul do Rio Grande do Sul. In: CONGRESSO BRASILEIRO DE QUALIDADE DO LEITE, 9., Goiânia. Inovação, sustentabilidade e oportunidades: anais. Curitiba: Conselho Brasileiro de Qualidade do Leite, 2022. p. 158-160. CBQL.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 5. | | HEPP, E. da S.; ANGELO, I. D. V.; ZANELA, M. B.; WEISSHEIMER, C. F.; SCHRAMM, R. C.; RIBEIRO, M. E. R. Incidência de staphylococcus coagulase negativo no rebanho do Sispel no ano de 2015. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 25.; SEMANA INTEGRADA DE ENSINO, PESQUISA E EXTENSÃO, 2., 2016, Pelotas. Anais... Pelotas: UFPel, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 6. | | RIBEIRO, M. E. R.; SANTOS, C. da S. dos; ZANELA, M. B.; SPALLONE, M. G.; GONZALEZ, H. de L.; MARTINS, P. R. G.; SCHRAMM, R. C. Ocorrência do Corynebacterium bovis em diferentes estações do ano na Região Sul do RS. In: SIMPÓSIO SOBRE SUSTENTABILIDADE DA PECUÁRIA LEITEIRA NA REGIÃO SUL DO BRASIL, 5, 2012: Sistemas de produção leiteira de base familiar como forma de fixação do homem no campo, Maringá. Anais...Maringa: Universidade Estadual de Maringá., SUL LEITE, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 7. | | ANGELO, I. D. V.; RIBEIRO, M. E. R.; ZANELA, M. B.; WEISSHEIMER, C. F.; OLIVEIRA, J. F. de; SCHRAMM, R. C.; ROSA, P. P. da. Mastite subclínica em um rebanho Jersey no Sul do Rio Grande do Sul 1. Incidência de agentes infecciosos. In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 26., 2016, Santa Maria. Cinquenta anos de zootecnia no Brasil: anais. Santa Maria: Sociedade Brasileira de Zootecnia, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 8. | | ANGELO, I. D. V.; RIBEIRO, M. E. R.; ZANELA, M. B.; WEISSHEIMER, C. F.; OLIVEIRA, J. F. de; SCHRAMM, R. C.; ROSA, P. P. da. Mastite subclínica em um rebanho Jersey no Sul do Rio Grande do Sul 2. Variação Sazonal. In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 26., 2016, Santa Maria. Cinquenta anos de zootecnia no Brasil: anais. Santa Maria: Sociedade Brasileira de Zootecnia, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 9. | | KOLLING, G. J.; RIBEIRO, M. E. R.; ZANELA, M. B.; WEISSHEIMER, C. F.; SCHRAMM, R. C.; STUMPF, M. T.; SANTOS, C. da S. dos. Monitoramento da mastite em um rebanho Jersey no Sul do Rio Grande do Sul - Resultados finais de 2011. In: CONGRESSO INTERNACIONAL DO LEITE, 11.; WORKSHOP SOBRE POLÍTICAS PÚBLICAS, 11.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 12., 2012, Goiânia. Anais... Juiz de Fora: Embrapa Gado de Leite, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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