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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
21/07/2000 |
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Data da última atualização: |
11/06/2024 |
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Autoria: |
CANTLIFFE, D. J.; NASCIMENTO, W. M.; SUNG, Y.; HUBER, D. J. |
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Afiliação: |
D. J. CATLIFFE, UNIVERSITY OF FLORIDA; WARLEY MARCOS NASCIMENTO, CNPH. |
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Título: |
Lettuce endosperm weakening: a role for endo-B-mannanase in seed germination at high temperature. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
In: BLACK, M.; BRADFORD, K.J.; VAZQUEZ-RAMOS, J., (Ed.). Seed biology: advances and applications. Wallingford: CAB, 2000. |
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Páginas: |
p. 277-285. |
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ISBN: |
0-85199-404-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Under high temperatures, seed germination of most lettuce genotypes can be erratic or completely inhibited. Weakening of the endosperm layer is a prerequisite to radicle protrusion at high temperatures. Enzyme-mediated degradation of endosperm cell walls may be a crucial factor for lettuce germination at high temperatures. The galactomannan polysaccharides in lettuce endosperm cell wall are mobilized by endo-b-mannanase. The involvement of endo-b-mannanase during germination of lettuce seeds at high temperature (35°C) was investigated by gel-diffusion assay. Genotype, seed maturation temperature, and seed priming enhanced lettuce seed germination at high temperature. Less force to penetrate the endosperm was required by seeds of thermotolerant genotypes or primed seeds, and structural alterations of endosperm in the micropylar area were also observed before radicle protrusion. Higher endo-b-mannanase activity in lettuce endosperm before radicle protrusion was verified in these situations. Moreover, at high temperature, thermotolerant genotypes produced more ethylene, more endo-b-mannanase, and germinated better than thermosensitive genotypes. An inhibitor of ethylene action, silver thiosulphate, inhibited both mannanase activity and germination. A precursor of ethylene, ACC, induced endo-b-mannanase activity and germination of thermosensitive ‘Dark Green Boston’ at 35°C. These results suggest that increased endo-b-mannanase activity might contribute to lettuce endosperm weakening, especially at high temperatures. MenosUnder high temperatures, seed germination of most lettuce genotypes can be erratic or completely inhibited. Weakening of the endosperm layer is a prerequisite to radicle protrusion at high temperatures. Enzyme-mediated degradation of endosperm cell walls may be a crucial factor for lettuce germination at high temperatures. The galactomannan polysaccharides in lettuce endosperm cell wall are mobilized by endo-b-mannanase. The involvement of endo-b-mannanase during germination of lettuce seeds at high temperature (35°C) was investigated by gel-diffusion assay. Genotype, seed maturation temperature, and seed priming enhanced lettuce seed germination at high temperature. Less force to penetrate the endosperm was required by seeds of thermotolerant genotypes or primed seeds, and structural alterations of endosperm in the micropylar area were also observed before radicle protrusion. Higher endo-b-mannanase activity in lettuce endosperm before radicle protrusion was verified in these situations. Moreover, at high temperature, thermotolerant genotypes produced more ethylene, more endo-b-mannanase, and germinated better than thermosensitive genotypes. An inhibitor of ethylene action, silver thiosulphate, inhibited both mannanase activity and germination. A precursor of ethylene, ACC, induced endo-b-mannanase activity and germination of thermosensitive ‘Dark Green Boston’ at 35°C. These results suggest that increased endo-b-mannanase activity might contribute to lettuce endosperm weak... Mostrar Tudo |
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Palavras-Chave: |
Endosperms; High temperature. |
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Thesagro: |
Alface; Atividade Enzimática; Dormência; Endosperma; Germinação; Lactuca Sativa; Semente; Temperatura. |
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Thesaurus Nal: |
dormancy; enzyme activity; germination; lettuce; seeds. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02576naa a2200361 a 4500
001 1767626
005 2024-06-11
008 2000 bl uuuu u00u1 u #d
020 $a0-85199-404-0
100 1 $aCANTLIFFE, D. J.
245 $aLettuce endosperm weakening$ba role for endo-B-mannanase in seed germination at high temperature.$h[electronic resource]
260 $c2000
300 $ap. 277-285.
520 $aUnder high temperatures, seed germination of most lettuce genotypes can be erratic or completely inhibited. Weakening of the endosperm layer is a prerequisite to radicle protrusion at high temperatures. Enzyme-mediated degradation of endosperm cell walls may be a crucial factor for lettuce germination at high temperatures. The galactomannan polysaccharides in lettuce endosperm cell wall are mobilized by endo-b-mannanase. The involvement of endo-b-mannanase during germination of lettuce seeds at high temperature (35°C) was investigated by gel-diffusion assay. Genotype, seed maturation temperature, and seed priming enhanced lettuce seed germination at high temperature. Less force to penetrate the endosperm was required by seeds of thermotolerant genotypes or primed seeds, and structural alterations of endosperm in the micropylar area were also observed before radicle protrusion. Higher endo-b-mannanase activity in lettuce endosperm before radicle protrusion was verified in these situations. Moreover, at high temperature, thermotolerant genotypes produced more ethylene, more endo-b-mannanase, and germinated better than thermosensitive genotypes. An inhibitor of ethylene action, silver thiosulphate, inhibited both mannanase activity and germination. A precursor of ethylene, ACC, induced endo-b-mannanase activity and germination of thermosensitive ‘Dark Green Boston’ at 35°C. These results suggest that increased endo-b-mannanase activity might contribute to lettuce endosperm weakening, especially at high temperatures.
650 $adormancy
650 $aenzyme activity
650 $agermination
650 $alettuce
650 $aseeds
650 $aAlface
650 $aAtividade Enzimática
650 $aDormência
650 $aEndosperma
650 $aGerminação
650 $aLactuca Sativa
650 $aSemente
650 $aTemperatura
653 $aEndosperms
653 $aHigh temperature
700 1 $aNASCIMENTO, W. M.
700 1 $aSUNG, Y.
700 1 $aHUBER, D. J.
773 $tIn: BLACK, M.; BRADFORD, K.J.; VAZQUEZ-RAMOS, J., (Ed.). Seed biology: advances and applications. Wallingford: CAB, 2000.
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Embrapa Hortaliças (CNPH) |
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| 10. |  | AVIDOS, M. F. D. (Org.). Cenargenda online. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. 1 CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 247).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | FOLLE, S. M.; FERREIRA, M. A.; FORTES, L. S.; AVIDOS, M. F. D.; CAJUEIRO, E. V. de M.; CONTE, A. C.; TIGANO, M. S.; MACHADO, M. I. P. Atividades do Núcleo de Gestão da Informação da Embrapa Recursos Genéticos e Biotecnologia. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. 25 p. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 267).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 25 | |
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