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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
31/03/1993 |
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Data da última atualização: |
05/03/2024 |
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Autoria: |
RESENDE, R. de O.; HAAN, P. de; VOSSEN, E. van de; AVILA, A. C. de; GOLDBACH, R.; PETERS, D. |
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Afiliação: |
RENATO DE OLIVEIRA RESENDE; PETER DE HAAN; EDWIN VAN DE VOSSEN; ANTONIO CARLOS DE AVILA, CNPH; ROB GOLDBACH; DICK PETERS. |
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Título: |
Defective interfering L RNA segments of tomato spotted wilt virus retain both virus genome termini and have extensive internal deletions. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Journal of General Virology, v. 73, p. 2509-2516, 1992. |
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DOI: |
https://doi.org/10.1099/0022-1317-73-10-2509 |
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Idioma: |
Inglês |
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Conteúdo: |
Defective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another containing the repeated sequences, during the replication of the viral complementary L RNA segment. MenosDefective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another conta... Mostrar Tudo |
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Palavras-Chave: |
Caracterizacao; Characterization; DI L RNA; Generation; Geracao; Maintenance; TSWV; Vira-Cabeca. |
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Thesagro: |
Manutenção. |
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Thesaurus Nal: |
Tomato spotted wilt virus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02548naa a2200313 a 4500
001 1749565
005 2024-03-05
008 1992 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1099/0022-1317-73-10-2509$2DOI
100 1 $aRESENDE, R. de O.
245 $aDefective interfering L RNA segments of tomato spotted wilt virus retain both virus genome termini and have extensive internal deletions.$h[electronic resource]
260 $c1992
520 $aDefective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another containing the repeated sequences, during the replication of the viral complementary L RNA segment.
650 $aTomato spotted wilt virus
650 $aManutenção
653 $aCaracterizacao
653 $aCharacterization
653 $aDI L RNA
653 $aGeneration
653 $aGeracao
653 $aMaintenance
653 $aTSWV
653 $aVira-Cabeca
700 1 $aHAAN, P. de
700 1 $aVOSSEN, E. van de
700 1 $aAVILA, A. C. de
700 1 $aGOLDBACH, R.
700 1 $aPETERS, D.
773 $tJournal of General Virology$gv. 73, p. 2509-2516, 1992.
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Embrapa Hortaliças (CNPH) |
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
19/08/2021 |
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Data da última atualização: |
19/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
RODRIGUES, A. L.; VILLA, P. M.; RODRIGUES, L. N. |
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Afiliação: |
ALISSON LOPES RODRIGUES; PEDRO MANUEL VILLA; LINEU NEIVA RODRIGUES, CPAC. |
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Título: |
Water balance estimate of beans using a dynamic systems model based on crop coefficient (kc) variation. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Engenharia na Agricultura, v. 29, p. 81-89, 2021. |
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Páginas: |
p. 81-89 |
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DOI: |
DOI: 10.13083/reveng.v29i1.9767 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT The crop coefficient (Kc) is one of the most important parameters for studying the water balance, which aims to assess the relationship between growth and production dynamics and the management of water resources in irrigation systems. Thus, in this study, the effect of Kc variation on the water balance was evaluated throughout the bean crop cycle in Northeast Brazil, using a dynamic systems model. The effect of Kc variation scenarios on the water balance in the bean crop in Northeastern Brazil was simulated using the Vensim program. The soil water storage was used as state variable, input flow variable and outflow of water in the soil, and auxiliary variables that influence system flows were also considered. Thus, four simulation scenarios were performed changing values of the bean crop coefficient (Kc = 0.62; Kc = 0.72; Kc = 0.82; Kc = 0.92). The variation in soil water storage showed negative values in most phenological phases, showing significant differences between Kc conditions, depending mainly on the precipitation dynamics. |
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Palavras-Chave: |
Déficit hídrico; Modelo hidrológico. |
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Thesagro: |
Evapotranspiração; Phaseolus Vulgaris. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01739naa a2200217 a 4500
001 2133741
005 2021-08-19
008 2021 bl uuuu u00u1 u #d
024 7 $aDOI: 10.13083/reveng.v29i1.9767$2DOI
100 1 $aRODRIGUES, A. L.
245 $aWater balance estimate of beans using a dynamic systems model based on crop coefficient (kc) variation.$h[electronic resource]
260 $c2021
300 $ap. 81-89
520 $aABSTRACT The crop coefficient (Kc) is one of the most important parameters for studying the water balance, which aims to assess the relationship between growth and production dynamics and the management of water resources in irrigation systems. Thus, in this study, the effect of Kc variation on the water balance was evaluated throughout the bean crop cycle in Northeast Brazil, using a dynamic systems model. The effect of Kc variation scenarios on the water balance in the bean crop in Northeastern Brazil was simulated using the Vensim program. The soil water storage was used as state variable, input flow variable and outflow of water in the soil, and auxiliary variables that influence system flows were also considered. Thus, four simulation scenarios were performed changing values of the bean crop coefficient (Kc = 0.62; Kc = 0.72; Kc = 0.82; Kc = 0.92). The variation in soil water storage showed negative values in most phenological phases, showing significant differences between Kc conditions, depending mainly on the precipitation dynamics.
650 $aEvapotranspiração
650 $aPhaseolus Vulgaris
653 $aDéficit hídrico
653 $aModelo hidrológico
700 1 $aVILLA, P. M.
700 1 $aRODRIGUES, L. N.
773 $tEngenharia na Agricultura$gv. 29, p. 81-89, 2021.
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