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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
09/04/2010 |
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Data da última atualização: |
25/09/2019 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
COSTA, S. H. F.; ANDRADE, E. R.; SILVA, J. R. V.; AMORIM, C. A.; LOBO, R. N. B.; OHASHI, O. M.; FIGUEIREDO, J. R. |
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Afiliação: |
UECe, Fortaleza, CE.; UECE; UECe; UFSM, Santa Maria, RS; RAIMUNDO NONATO BRAGA LOBO, CNPC; UFPA, Belém, PA; UECe. |
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Título: |
Preservation of goat preantral follicles enclosed in ovarian tissue in saline or TCM 199 solutions. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Small Ruminant Research, v. 58, n. 2, p. 189-193, May, 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of the study was to evaluate the effect of saline and TCM 199 solutions at different temperatures and incubation times on the morphology of goat preantral follicles. At the slaughterhouse the ovaries from does (n = 5) was divided into 18 fragments. The ovarian samples were randomly allocated to a saline or TCM 199 solution and stored at 4, 20 or 39 °C for 4, 12 or 24 h. The histological analysis showed that of normal follicles stored in a saline solution at 4 °C for up to 24 h was similar to that of TCM 199. However, the average number of morphologically normal preantral follicles stored in saline solution at 20 °C for 12 and 24 h and at 39 °C for all incubation periods was significantly (P < 0.05) lower when compared to those in TCM 199. Saline solution or TCM 199 can be used with the same effectiveness in preserving goat preantral follicles at 4 °C for storage periods of up to 24 h. However, for the preservation of goat preantral follicles at higher temperatures, TCM 199 is recommended. |
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Palavras-Chave: |
Folículo pré-antral; Goat; Preantral follicle; Preservação; Preservation. |
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Thesagro: |
Caprino; Histologia; Ovário. |
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Thesaurus Nal: |
Animal reproduction; Histology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01904naa a2200313 a 4500
001 1663707
005 2019-09-25
008 2005 bl uuuu u00u1 u #d
100 1 $aCOSTA, S. H. F.
245 $aPreservation of goat preantral follicles enclosed in ovarian tissue in saline or TCM 199 solutions.$h[electronic resource]
260 $c2005
520 $aThe aim of the study was to evaluate the effect of saline and TCM 199 solutions at different temperatures and incubation times on the morphology of goat preantral follicles. At the slaughterhouse the ovaries from does (n = 5) was divided into 18 fragments. The ovarian samples were randomly allocated to a saline or TCM 199 solution and stored at 4, 20 or 39 °C for 4, 12 or 24 h. The histological analysis showed that of normal follicles stored in a saline solution at 4 °C for up to 24 h was similar to that of TCM 199. However, the average number of morphologically normal preantral follicles stored in saline solution at 20 °C for 12 and 24 h and at 39 °C for all incubation periods was significantly (P < 0.05) lower when compared to those in TCM 199. Saline solution or TCM 199 can be used with the same effectiveness in preserving goat preantral follicles at 4 °C for storage periods of up to 24 h. However, for the preservation of goat preantral follicles at higher temperatures, TCM 199 is recommended.
650 $aAnimal reproduction
650 $aHistology
650 $aCaprino
650 $aHistologia
650 $aOvário
653 $aFolículo pré-antral
653 $aGoat
653 $aPreantral follicle
653 $aPreservação
653 $aPreservation
700 1 $aANDRADE, E. R.
700 1 $aSILVA, J. R. V.
700 1 $aAMORIM, C. A.
700 1 $aLOBO, R. N. B.
700 1 $aOHASHI, O. M.
700 1 $aFIGUEIREDO, J. R.
773 $tSmall Ruminant Research$gv. 58, n. 2, p. 189-193, May, 2005.
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