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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
08/03/2010 |
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Data da última atualização: |
21/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FUGANTI, R.; MACHADO, M. de F. P. da S.; LOPES, V. S.; SILVA, J. F. V.; ARIAS, C. A. A.; MARIN, S. R. R.; BINNECK, E.; ABDELNOOR, R. V.; MARCELINO, F. C.; NEPOMUCENO, A. L. |
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Afiliação: |
RENATA FUGANTI, UEM; MARIA DE FÁTIMA PIRES DA SILVA MACHADO, UEM; VALÉRIA STEFANIA LOPES, UNIFIL; JOAO FLAVIO VELOSO SILVA, CPAMT; CARLOS ALBERTO ARRABAL ARIAS, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; ELISEU BINNECK, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
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Título: |
Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Journal of Biomedicine and Biotechnology, 847673, 2010. |
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Páginas: |
9 p. |
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DOI: |
10.1155/2010/847673 |
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Idioma: |
Inglês |
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Conteúdo: |
During earlier experiments, an SSR molecularmarker (176 SoyHSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene. It was also detected in this region that resistant genotypes presented insertions between AT(31) and AT(33) in size and susceptible genotypes, AT(9). Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes. A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes. A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more AT(n) insertions. These results suggest that the number ofAT(n) insertions inside this promoter region couldmodulate up or down gene levels. Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of AT(n) insertions. |
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Palavras-Chave: |
Marcadores moleculares; Nematóide de galha; Soybean. |
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Thesagro: |
Meloidogyne Javanica; Nematóide; Soja. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02076naa a2200325 a 4500
001 1660121
005 2024-06-21
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1155/2010/847673$2DOI
100 1 $aFUGANTI, R.
245 $aSize of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels.$h[electronic resource]
260 $c2010
300 $a9 p.
520 $aDuring earlier experiments, an SSR molecularmarker (176 SoyHSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene. It was also detected in this region that resistant genotypes presented insertions between AT(31) and AT(33) in size and susceptible genotypes, AT(9). Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes. A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes. A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more AT(n) insertions. These results suggest that the number ofAT(n) insertions inside this promoter region couldmodulate up or down gene levels. Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of AT(n) insertions.
650 $aMeloidogyne Javanica
650 $aNematóide
650 $aSoja
653 $aMarcadores moleculares
653 $aNematóide de galha
653 $aSoybean
700 1 $aMACHADO, M. de F. P. da S.
700 1 $aLOPES, V. S.
700 1 $aSILVA, J. F. V.
700 1 $aARIAS, C. A. A.
700 1 $aMARIN, S. R. R.
700 1 $aBINNECK, E.
700 1 $aABDELNOOR, R. V.
700 1 $aMARCELINO, F. C.
700 1 $aNEPOMUCENO, A. L.
773 $tJournal of Biomedicine and Biotechnology, 847673, 2010.
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Embrapa Soja (CNPSO) |
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