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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/02/2010 |
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Data da última atualização: |
29/05/2023 |
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Tipo da produção científica: |
Artigo de Divulgação na Mídia |
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Autoria: |
FERREIRA, C. F.; VAN DER LEE, T. A. J.; QUIROS, O.; TE LINTEL, H.; ZAPATER, M.; CARLIER, J.; GOODWIN, S.; GUZMAN, M.; SOUZA JÚNIOR, M.; KEMA, G. H. J. |
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Afiliação: |
CLAUDIA FORTES FERREIRA, CNPMF; Theodoor Angelo Jozef Van Der Lee, PRIN; Olman Quirós, CORBANA; Bas Te Lintel Hekkert, PRIN; Marie Francoise Zapater, CIRAD; Jean Carlier, CIRAD; Stephen B. Goodwin; Mauricio Guzman, CORBANA; Manoel Teixeira Souza Júnior, PRIN; Gert H. J. Kema, PRIN. |
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Título: |
Development of VNTR markers to assess genetic diversity of Mycosphaerella fijiensis, the causal agent of black leaf streak disease in bananas (Musa spp.). |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
UNITED STATES DEPARTMENT OF AGRICULTURAL. Agricultural Research Service. Crop production and pest control research. |
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Idioma: |
Inglês |
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Notas: |
Disponível em: <http://www.ars.usda.gov/research/publications/publications.htm?seq_no_115=231337 >. Acesso em: 25 fev. 2010 |
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Conteúdo: |
Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana, commonly known as Black Sigatoka, is one of the most devastating plant pathogens. Black leaf streak was first discovered in Fiji during the 1960s and the pathogen still actively colonizes new areas where bananas are grown. Using the genome sequence of M. fijiensis isolate CIRAD86 (http://genome.jgi-psf.org/Mycfi1/Mycfi1.home.html), we identified Variable Number of Tandem Repeat (VNTR) markers using a bioinformatics pipeline. The VNTR screening resulted in 1528 candidate VNTR loci of which many were imperfect mono-, di-, or tri-nucleotide repeats. Sixteen VNTR markers were selected based on repeat type, repeat length and the number of units of the repeat. Primers for these 16 VNTR markers were tested on a set of M. fijiensis isolates and five VNTR markers that showed multiple alleles were tested on M. fijiensis field isolates and on progeny of the cross between isolates CIRAD86 and CIRAD 139a. Numbers of alleles identified varied from 2 to 5 per locus. The markers allowed high throughput and robust scoring on agarose gels and proved useful for genetic diversity and population genetic studies using basic molecular instrumentation. We envisage that this set will enable more detailed analyses of the large-scale migration pattern of this important pathogen as well as small-scale effects of fungicide treatments on the population diversity in banana plantations. |
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Palavras-Chave: |
Melhoramento genético. |
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Thesagro: |
Doença de Planta; Fungo. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02491naa a2200277 a 4500
001 1658910
005 2023-05-29
008 2008 bl uuuu u00u1 u #d
100 1 $aFERREIRA, C. F.
245 $aDevelopment of VNTR markers to assess genetic diversity of Mycosphaerella fijiensis, the causal agent of black leaf streak disease in bananas (Musa spp.).$h[electronic resource]
260 $c2008
500 $aDisponível em: <http://www.ars.usda.gov/research/publications/publications.htm?seq_no_115=231337 >. Acesso em: 25 fev. 2010
520 $aMycosphaerella fijiensis, the causal agent of black leaf streak disease of banana, commonly known as Black Sigatoka, is one of the most devastating plant pathogens. Black leaf streak was first discovered in Fiji during the 1960s and the pathogen still actively colonizes new areas where bananas are grown. Using the genome sequence of M. fijiensis isolate CIRAD86 (http://genome.jgi-psf.org/Mycfi1/Mycfi1.home.html), we identified Variable Number of Tandem Repeat (VNTR) markers using a bioinformatics pipeline. The VNTR screening resulted in 1528 candidate VNTR loci of which many were imperfect mono-, di-, or tri-nucleotide repeats. Sixteen VNTR markers were selected based on repeat type, repeat length and the number of units of the repeat. Primers for these 16 VNTR markers were tested on a set of M. fijiensis isolates and five VNTR markers that showed multiple alleles were tested on M. fijiensis field isolates and on progeny of the cross between isolates CIRAD86 and CIRAD 139a. Numbers of alleles identified varied from 2 to 5 per locus. The markers allowed high throughput and robust scoring on agarose gels and proved useful for genetic diversity and population genetic studies using basic molecular instrumentation. We envisage that this set will enable more detailed analyses of the large-scale migration pattern of this important pathogen as well as small-scale effects of fungicide treatments on the population diversity in banana plantations.
650 $aDoença de Planta
650 $aFungo
653 $aMelhoramento genético
700 1 $aVAN DER LEE, T. A. J.
700 1 $aQUIROS, O.
700 1 $aTE LINTEL, H.
700 1 $aZAPATER, M.
700 1 $aCARLIER, J.
700 1 $aGOODWIN, S.
700 1 $aGUZMAN, M.
700 1 $aSOUZA JÚNIOR, M.
700 1 $aKEMA, G. H. J.
773 $tUNITED STATES DEPARTMENT OF AGRICULTURAL. Agricultural Research Service. Crop production and pest control research.
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| 6. |  | BASTOS, T. X. Clima. In: DUARTE, M. de L. R. Cultivo da pimenteira-do-reino na Região Norte. Belém, PA: Embrapa Amazônia Oriental, 2004. p. 17-21. (Embrapa Amazônia Oriental. Sistemas de produção, 1).| Tipo: Capítulo em Livro Técnico-Científico |
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