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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
08/02/2010 |
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Data da última atualização: |
24/09/2019 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
CRUZ, J. C. M.; GOUVEIA, A. M. G.; SOUZA, K. C. de; BRAZ, G. F.; TEIXEIRA, B. M.; HEINEMANN, M. B.; LEITE, R. C.; REIS, J. K. P.; PINHEIRO, R. R.; ANDRIOLI, A. |
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Afiliação: |
Universidade Federal de Minas Gerias (UFMG), Belo Horizonte, MG; Aurora Maria Guimarães Gouveia, Universidade Federal de Minas Gerias (UFMG); Kelma Costa de Souza, Pós-graduanda Universidade Estadual Vale do Acaraú - UVA, Sobral, CE; Universidade Federal de Minas Gerias (UFMG); Universidade Federal de Minas Gerias (UFMG); Universidade Federal de Minas Gerias (UFMG); Universidade Federal de Minas Gerias (UFMG); Universidade Federal de Minas Gerias (UFMG); RAYMUNDO RIZALDO PINHEIRO, CNPC; ALICE ANDRIOLI, CNPC. |
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Título: |
Caprine arthritis-encephalitis virus (CAEV) detection in semen of endangered goat breeds by nested polymerase chain reaction. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Small Ruminant Research, v. 85, n. 2/3, p. 149-152, 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
Semen and peripheral blood mononuclear cells (PBMCs) samples of four naturally infected, four experimentally infected (endangered breeds) and four non-infected bucks (endangered breeds) were evaluated for the presence of CAEV proviral-DNA by nested polymerase chain reaction (n-PCR). Three out of the eight PBMC samples from infected bucks were positive for CAEV-DNA and four out of the eight semen samples were positive for CAEV proviral-DNA. This is the first report describing the presence of CAEV proviral-DNA in semen from seropositive Anglo-Nubian, Moxotó and Canindé bucks, providing useful information towards the design of efficient methods to prevent CAEV dissemination in the endangered goat livestock genetic resources in Brazil. |
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Palavras-Chave: |
Artrite encefalite caprina a vírus; CAEV; PCR; Reação; Reação de polimerase em cadeia. |
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Thesagro: |
Caprino; Sêmen. |
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Thesaurus Nal: |
Brazil; Caprine arthritis encephalitis virus; Caprine arthritis-encephalitis; Goat diseases; Polymerase chain reaction. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01862naa a2200373 a 4500 001 1657144 005 2019-09-24 008 2009 bl uuuu u00u1 u #d 100 1 $aCRUZ, J. C. M. 245 $aCaprine arthritis-encephalitis virus (CAEV) detection in semen of endangered goat breeds by nested polymerase chain reaction.$h[electronic resource] 260 $c2009 520 $aSemen and peripheral blood mononuclear cells (PBMCs) samples of four naturally infected, four experimentally infected (endangered breeds) and four non-infected bucks (endangered breeds) were evaluated for the presence of CAEV proviral-DNA by nested polymerase chain reaction (n-PCR). Three out of the eight PBMC samples from infected bucks were positive for CAEV-DNA and four out of the eight semen samples were positive for CAEV proviral-DNA. This is the first report describing the presence of CAEV proviral-DNA in semen from seropositive Anglo-Nubian, Moxotó and Canindé bucks, providing useful information towards the design of efficient methods to prevent CAEV dissemination in the endangered goat livestock genetic resources in Brazil. 650 $aBrazil 650 $aCaprine arthritis encephalitis virus 650 $aCaprine arthritis-encephalitis 650 $aGoat diseases 650 $aPolymerase chain reaction 650 $aCaprino 650 $aSêmen 653 $aArtrite encefalite caprina a vírus 653 $aCAEV 653 $aPCR 653 $aReação 653 $aReação de polimerase em cadeia 700 1 $aGOUVEIA, A. M. G. 700 1 $aSOUZA, K. C. de 700 1 $aBRAZ, G. F. 700 1 $aTEIXEIRA, B. M. 700 1 $aHEINEMANN, M. B. 700 1 $aLEITE, R. C. 700 1 $aREIS, J. K. P. 700 1 $aPINHEIRO, R. R. 700 1 $aANDRIOLI, A. 773 $tSmall Ruminant Research$gv. 85, n. 2/3, p. 149-152, 2009.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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