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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/02/2008 |
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Data da última atualização: |
24/07/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DIE, J. V.; DITA, M. A.; KRAJINSKI, F.; GONZÁLEZ-VERDEJO, C. I.; RUBIALES, D.; MORENO, M. T.; ROMÁN, B. |
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Afiliação: |
José Vicente Die, IFAPA; Miguel Angel Dita, CNPMF; Franziska Krajinski, Universitãt Hannover; Clara I. González-Verdejo, IFAPA; Diego Rubiales, CSIC; Maria Teresa Moreno, IFAPA; Belén Román, IFAPA. |
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Título: |
Identification by suppression subtractive hybridization and expression analysis of Medicago truncatula putative defence genes in response to Orobanche crenata parasitization. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Physiological and Molecular Plant Pathology, v. 70, p. 49-59, 2007. |
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Idioma: |
Inglês |
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Conteúdo: |
Crenate broomrape (Orobanche crenata) is the major constraint for pea and faba bean production in the Mediterranean region. In this study, a systematic sequencing of expressed sequence tags (ESTs) was chosen to obtain a first global picture of the assembly of genes involved in defence response. A cDNA-library was established by suppression subtractive hybridization in the model legume Medicago truncatula infected by O. crenata in order to identify a large number of host plant ESTs. Eighty-one presumably up-regulated genes were identified and classified in functional categories. EST-annotations showed homologies to a number of well-characterized genes. Most of the proteins encoded by these genes, are already known in defence in M. truncatula, such as genes related to the JA pathway or involved in cell wall modifications. A notable number of the ESTs, however, were derived from novel genes not matching entries of the large-scale M. truncatula sequences collections. Expression analyses by quantitative RT-PCR of 20 genes corresponding to different functional categories showed high expression levels, supporting their involvement in the defence response. |
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Palavras-Chave: |
Real-time RT-PCR; SSH. |
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Thesaurus Nal: |
Medicago truncatula; Orobanche crenata. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01959naa a2200241 a 4500
001 1654638
005 2023-07-24
008 2007 bl uuuu u00u1 u #d
100 1 $aDIE, J. V.
245 $aIdentification by suppression subtractive hybridization and expression analysis of Medicago truncatula putative defence genes in response to Orobanche crenata parasitization.$h[electronic resource]
260 $c2007
520 $aCrenate broomrape (Orobanche crenata) is the major constraint for pea and faba bean production in the Mediterranean region. In this study, a systematic sequencing of expressed sequence tags (ESTs) was chosen to obtain a first global picture of the assembly of genes involved in defence response. A cDNA-library was established by suppression subtractive hybridization in the model legume Medicago truncatula infected by O. crenata in order to identify a large number of host plant ESTs. Eighty-one presumably up-regulated genes were identified and classified in functional categories. EST-annotations showed homologies to a number of well-characterized genes. Most of the proteins encoded by these genes, are already known in defence in M. truncatula, such as genes related to the JA pathway or involved in cell wall modifications. A notable number of the ESTs, however, were derived from novel genes not matching entries of the large-scale M. truncatula sequences collections. Expression analyses by quantitative RT-PCR of 20 genes corresponding to different functional categories showed high expression levels, supporting their involvement in the defence response.
650 $aMedicago truncatula
650 $aOrobanche crenata
653 $aReal-time RT-PCR
653 $aSSH
700 1 $aDITA, M. A.
700 1 $aKRAJINSKI, F.
700 1 $aGONZÁLEZ-VERDEJO, C. I.
700 1 $aRUBIALES, D.
700 1 $aMORENO, M. T.
700 1 $aROMÁN, B.
773 $tPhysiological and Molecular Plant Pathology$gv. 70, p. 49-59, 2007.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 7 | |
| 2. |  | RIBEIRO, M. M.; SANTOS, A. B. dos; OLIVEIRA, L. S.; ABE, V. Y.; CASTANHO, F. M.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Análises de estrutura e indução da família de genes MLO de soja sob infecção por Phakopsora pachyrhizi. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8, 2023, Florianópolis, SC. Anais... Florianópolis: SBG, 2023. resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 3. | | UTIYAMA, A. S.; ABE, V. Y.; DARBEN, L. M.; CARVALHO, K.; CARVALHO, M. C. C. G. de; MARCELINO-GUIMARÃES, F. C. Avaliação funcional de proteínas candidatas a efetores de Phakopsora pachyrhizi por meio da análise de espécies reativas a oxigênio (ROS) e deposição de calose. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 13., 2018, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2018. p. 38-45.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 4. | | CARVALHO, K.; ABE, V. Y.; DARBEN, L. M.; RINCÃO, M. P.; QI, M.; UTIYAMA, A. S.; CARVALHO, M. C. C. G.; LOPES, I. de O. N.; ABDELNOOR, R. V.; WHITHAM, S. A.; MARCELINO-GUIMARÃES, F. C. Host induced gene silencing in P. pachyrhizi effectors interfere in virulence of soybean attenuating fungal pathogenicity. In: IS-MPMI Congress, 18., 2019, Glasgow. [Abstracts...]. [S. l.: s. n.], 2019.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 5. | | BUENO, T. V.; FONTES, P. P.; ABE, V. Y.; UTIYAMA, A. S.; SENRA, R. L.; OLIVEIRA, L. S.; SANTOS, A. B. dos; FERREIRA, E. G. C.; DARBEN, L. M.; OLIVEIRA, A. B. de; ABDELNOOR, R. V.; WHITHAM, S. A.; FIETTO, L. G.; MARCELINO-GUIMARÃES, F. C. A Phakopsora pachyrhizi Effector Suppresses PAMP-Triggered Immunity and Interacts with a Soybean Glucan Endo-1,3-β-Glucosidase to Promote Virulence. MOLECULAR PLANT-MICROBE INTERACTIONS, v. 35, n. 9, p. 779-790, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 6. | | SANTOS, I, M. O. dos; ABE, V. Y.; CARVALHO, K. de; BARAZETTI, A. R.; SIMIONATO, A. S.; PEGA, G. E. de A.; MATIS, S. H.; CANO, B. G.; CELY, M. V. T.; MARCELINO-GUIMARÃES, F. C.; CHRYSSAFIDS, A. L.; ANDRADE, G. Secondary metabolites of Pseudomonas aeruginosa lv strain decrease asian soybean rust severity in experimentally infected plants. Plants, v. 10, n. 8, 1495, 2021. 12 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Soja. |
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| 7. | | CASTANHO, F. M.; COSTA, B. L. C. da; ABE, V. Y.; YOKOYAMA, A.; DARBEN, L. M.; OLIVEIRA, L. S.; FERREIRA, E. G. C.; LOPES, I. de O. N.; CARVALHO, M. C. DA C. G. de; BALBI-PEÑA, M. I.; MARCELINO-GUIMARÃES, F. C. Variability and functional characterization of the Phakopsora pachyrhizi Egh16-like effectors. Genetics and Molecular Biology, v. 47, n. 3, e20230192, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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