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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
10/01/2008 |
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Data da última atualização: |
29/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BANI HASHEMIAN, S. M.; BARBOSA, C. de J.; PINA, J. A.; DURAN-VILA, N. |
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Afiliação: |
S. M. Bani Hashemian, IVIA; Cristiane de Jesus Barbosa, CNPMF; J. A. Pina, Servicio de Semillad y Plantas de Vivero; N. Duran-Vila, IVIA. |
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Título: |
Desert lime (Eremocitrus glauca) appears to be resistant to viroid infection. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: CONFERENCE OF THE INTERNATIONAL ORGANIZATION OF CITRUS VIROLOGISTS, 17., 2007, Adana. Program & Abstracts... Adana: [s.n.], 2007. p. 104. |
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Idioma: |
Inglês |
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Conteúdo: |
Desert lime (Eremocitrus glauca) is a small bushy tree confined to inland areas of Australia, usually on heavy, clay soils. Because of its habitat, it is of interest as a rootstock to possibly extend the range of commercial citrus crops. Because of its potential use for rootstock breeding, studies were conducted to evaluate its sensitivity to viroid infection. Self-rooted plants were obtained by in vitro micropropagation and were graft-inoculated (two plants each) with either Citrus exocortis viroid (CEVd), Citrus bent leaf viroid (CBLVd), Citrus viroid III (CVd-III), Citrus viroid IV (CVd.IV), Citrus viroid V (CVd-V) or two strains (lla, llc) of Hop stunt viroid (HSVd). None of the inoculated viroids were detected by Northern blot hybridization and RT-PCR, 18 months post inoculation. Four plants of desert lime, two of which had been obtained by in vitro micropropagation and the other two by grafting on Rough lemon rootstock were graft-inoculated with an artificial mixture of the same viroids (CEVd, CBLVd, CVd-III, CVd-IV, CVd-V and two strains of HSVd). The graft-propagated plants were inoculated into the scion and trained to alow the growth of a sucker from the rootstock. Two micropropagated and two grafted plants were also maintained as non-inoculated controls. None of the inoculated viroids were detected in the desert lime plants regardless of whether they were growing on their own roots (micropropagated) or grafted on the rough lemon rootstock. However, analysis of the suckers growing from the roostock revealed that all the viroids moved from the inoculation site within the scion to the rootstock where they reached detectable titters. These results suggest that, in contrast with other species, desert lime acts as a true resistant genotype, but it allows long distance movement of citrus viroids. MenosDesert lime (Eremocitrus glauca) is a small bushy tree confined to inland areas of Australia, usually on heavy, clay soils. Because of its habitat, it is of interest as a rootstock to possibly extend the range of commercial citrus crops. Because of its potential use for rootstock breeding, studies were conducted to evaluate its sensitivity to viroid infection. Self-rooted plants were obtained by in vitro micropropagation and were graft-inoculated (two plants each) with either Citrus exocortis viroid (CEVd), Citrus bent leaf viroid (CBLVd), Citrus viroid III (CVd-III), Citrus viroid IV (CVd.IV), Citrus viroid V (CVd-V) or two strains (lla, llc) of Hop stunt viroid (HSVd). None of the inoculated viroids were detected by Northern blot hybridization and RT-PCR, 18 months post inoculation. Four plants of desert lime, two of which had been obtained by in vitro micropropagation and the other two by grafting on Rough lemon rootstock were graft-inoculated with an artificial mixture of the same viroids (CEVd, CBLVd, CVd-III, CVd-IV, CVd-V and two strains of HSVd). The graft-propagated plants were inoculated into the scion and trained to alow the growth of a sucker from the rootstock. Two micropropagated and two grafted plants were also maintained as non-inoculated controls. None of the inoculated viroids were detected in the desert lime plants regardless of whether they were growing on their own roots (micropropagated) or grafted on the rough lemon rootstock. However, analysis of the ... Mostrar Tudo |
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Thesagro: |
Fruta Cítrica; Vírus. |
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Categoria do assunto: |
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Marc: |
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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