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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
16/08/1999 |
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Data da última atualização: |
16/05/2023 |
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Autoria: |
FITCH, M. M. M.; MANSHARDT, R. M. |
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Afiliação: |
Department of Horticulture, University of Hawaii, 3190 Maile Way, Honolulu, HI 96822, USA. |
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Título: |
Somatic embryogenesis and plant regeneration from immature zygotic embryos of papaya (Carica papaya L.). |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Plant Cell Reports, v.9, n.6, p.320-324, 1990. |
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Idioma: |
Inglês |
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Conteúdo: |
Immature zygotic embryos from openpollinated and selfed Carica papaya L. fruits, 90 to 114 days post-anthesis, produced 2 to 20 somatic embryos on apical domes, cotyledonary nodes, and radicle meristems after culture for three weeks on half-strength Murashige and Skoog (MS) medium supplemented with 0.1 to 25 mg 1-1 2,4-dichlorophenoxyacetic acid (2,4-D), 400 mg 1-1 glutamine, and 6% sucrose. After six weeks of culture, about 40 to 50% of the zygotic embryos had become embryodenic, and each embryogenic embryo yielded hundreds of somatic embryos within five months of culture on media supplemented with 2,4-D. Somatic embryos matured on half-strength MS medium, germinated on MS medium containing 5 mg 1-1 kinetin, and grew large enough for greenhouse culture on MS medium. Shoots were rooted in vermiculite and grown in the greenhouse. |
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Palavras-Chave: |
2; Carica papaya L. |
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Thesaurus Nal: |
somatic embryogenesis; tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01391naa a2200181 a 4500
001 1648256
005 2023-05-16
008 1990 bl uuuu u00u1 u #d
100 1 $aFITCH, M. M. M.
245 $aSomatic embryogenesis and plant regeneration from immature zygotic embryos of papaya (Carica papaya L.).$h[electronic resource]
260 $c1990
520 $aImmature zygotic embryos from openpollinated and selfed Carica papaya L. fruits, 90 to 114 days post-anthesis, produced 2 to 20 somatic embryos on apical domes, cotyledonary nodes, and radicle meristems after culture for three weeks on half-strength Murashige and Skoog (MS) medium supplemented with 0.1 to 25 mg 1-1 2,4-dichlorophenoxyacetic acid (2,4-D), 400 mg 1-1 glutamine, and 6% sucrose. After six weeks of culture, about 40 to 50% of the zygotic embryos had become embryodenic, and each embryogenic embryo yielded hundreds of somatic embryos within five months of culture on media supplemented with 2,4-D. Somatic embryos matured on half-strength MS medium, germinated on MS medium containing 5 mg 1-1 kinetin, and grew large enough for greenhouse culture on MS medium. Shoots were rooted in vermiculite and grown in the greenhouse.
650 $asomatic embryogenesis
650 $atissue culture
653 $a2
653 $aCarica papaya L
700 1 $aMANSHARDT, R. M.
773 $tPlant Cell Reports$gv.9, n.6, p.320-324, 1990.
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| 5. |  | OLIVEIRA, R. C.; CARNEIRO, T. R.; MASSARO, R. A. M.; FAUSTINO, C. C.; FERNANDES, O. A.; PRATISSOLI, D.; CRUZ, I. Influência de diferentes técnicas de manipulação de posturas de Spodoptera frugiperda (Lepidoptera noctuidae) sobre aspectos biológicos de Telenomus remus (Hymenoptera: scelionidae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 20., 2004, Gramado. Programa e resumos... Gramado: Sociedade Entomológica do Brasil, 2004. p. 274.| Tipo: Resumo em Anais de Congresso |
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| 6. | | OLIVEIRA, R. C. B. W.; RESENDE, M. A. de; VALÉRIO, H. M.; CALIGIORNE, R. B.; PAIVA, E. Genetic variation among pathogens causing "Helminthosporium" diseases of rice, maize and wheat. Fitopatologia Brasileira, Brasília, v. 27, n. 6, p. 639-643, nov./dez. 2002.| Biblioteca(s): Embrapa Milho e Sorgo. |
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