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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
05/08/1997 |
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Data da última atualização: |
17/05/2023 |
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Autoria: |
LOUZADA, E. S.; GROSSER, J. W.; GMITTER JUNIOR, F. G. |
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Afiliação: |
University of Florida, USA. |
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Título: |
Intergeneric somatic hybridization of sexually incompatible parents: Citrus sinensis and Atalantia ceylanica. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Plant Cell Reports, v.12, p.687-690, 1993. |
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Idioma: |
Inglês |
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Conteúdo: |
Protoplast fusion using polyethylene glycol (PEG) was conducted to combine Citrus sinensis (L.) Osbeck cv. 'Hamilin'sweet orange protoplast, isolated from nucellus-derived embryogenic callus with Atalantia ceylanica (Arn.) Oliv. leaf protoplast. Five plants regenerated from independent fusion events following protoplast culture were identified as intergeneric allotetraploid somatic hybrids of 'Hamilin'sweet orange and A. ceylanica, and confirmed by isozyme analysis and chromosome number determination in root tip cells (2n=4x=36). Two different types of leaf morphology were observed among the hybrids (normal and narrow), although no differences in chromosome number nor isozyme banding patterns were observed. This is the first report of the production of hybrid plants between these sexually incompatible genera. |
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Thesagro: |
Fruta Cítrica. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01319naa a2200157 a 4500
001 1647963
005 2023-05-17
008 1993 bl uuuu u00u1 u #d
100 1 $aLOUZADA, E. S.
245 $aIntergeneric somatic hybridization of sexually incompatible parents$bCitrus sinensis and Atalantia ceylanica.$h[electronic resource]
260 $c1993
520 $aProtoplast fusion using polyethylene glycol (PEG) was conducted to combine Citrus sinensis (L.) Osbeck cv. 'Hamilin'sweet orange protoplast, isolated from nucellus-derived embryogenic callus with Atalantia ceylanica (Arn.) Oliv. leaf protoplast. Five plants regenerated from independent fusion events following protoplast culture were identified as intergeneric allotetraploid somatic hybrids of 'Hamilin'sweet orange and A. ceylanica, and confirmed by isozyme analysis and chromosome number determination in root tip cells (2n=4x=36). Two different types of leaf morphology were observed among the hybrids (normal and narrow), although no differences in chromosome number nor isozyme banding patterns were observed. This is the first report of the production of hybrid plants between these sexually incompatible genera.
650 $aFruta Cítrica
700 1 $aGROSSER, J. W.
700 1 $aGMITTER JUNIOR, F. G.
773 $tPlant Cell Reports$gv.12, p.687-690, 1993.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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