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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
15/08/2001 |
Data da última atualização: |
15/08/2001 |
Autoria: |
FIGUEIREDO, J. O. de; PIO, R. M.; TEOFILO SOBRINHO, J.; LARANJEIRA, F. F. SALIBE, A. A. |
Afiliação: |
IAC; CNPq; UNESP. |
Título: |
Comportamento de quinze porta-enxertos para o tangot "Murcott' na Regiao de Porto Feliz, SP1 |
Ano de publicação: |
2001 |
Fonte/Imprenta: |
Revista Brasileira de Fruticultura, Jaboticabal-SP, v.23, n.1, p.147-151, 2001 |
ISSN: |
0100-2945 |
Idioma: |
Português |
Conteúdo: |
Foi analisado o comportamento de plantas de tangor 'Murcott', nucelar,em 15 porta-enxertos. O experimento foi instalado na fazenda Ana Maria, Porto Feliz-SP, em 1990. os porta-enxertos utilizados foram: tangelo'Orlando', laranja-'Caipira DAC', limoes-'Cravo'e 'Pook Ling Ming', trifoliatas 'Kryder 8-5' e EEL'e tangerinas-'Cleopatra', 'Sunki', 'Batangas', 'Oneco', 'Swatow', 'Szinkon', 'Cravo', 'Dancy', 'Suen Kat'. O delineamento estatistico utilizado foi o de blocos ao acaso, com uma planta por parcela e com dez repeticoes. As tangerinas-Gleopatra'e 'Suen kat'induziram as maiores copas maiores producoes por arvore. As menores producoes foram influenciadas pelo trifoliata 'EEL' e pela laranja-'Caipira DAC'. As menores copas foram proporcionadas pelos trifoliatase pela laranja-'Caipira DAC'. Os trifoliatas 'EEL' e 'Kryder 8-5'apresentaram-se incompativeis com a copa 'Murcott'. Nao foram observadas diferencas significativas para a maioria das variaveis relacionadas a qualidade dos frutos. |
Palavras-Chave: |
Citros; Productivity; Qualidade do fruto; Tree vigour; Vigor da planta. |
Thesagro: |
Produtividade. |
Thesaurus Nal: |
Citrus; fruit quality. |
Categoria do assunto: |
-- |
Marc: |
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
10/02/2014 |
Data da última atualização: |
08/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MOURÃO, M. de M.; BITAR, M.; LOBO, F. P.; PECONICK, A. P.; GRYNBERG, P.; PROSDOCIMI, F.; WAISBERG, M.; CERQUEIRA, G. C.; MACEDO, A. M.; MACHADO, C. R.; YOSHINO, T.; FRANCO, G. R. |
Afiliação: |
MARINA DE MORAES MOURÃO, Centro de Pesquisas René Rachou-Fiocruz; MAINÁ BITAR, UFMG; FRANCISCO PEREIRA LOBO, CNPTIA; ANA PAULA PECONICK, Ufla; PRISCILA GRYNBERG, UFMG; FRANCISCO PROSDOCIMI, UFRJ; MICHAEL WAISBERG, University of Virginia; GUSTAVO COUTINHO CERQUEIRA, Broad Institute of Massachusetts Institute of Technology and Harvard; ANDRÉA MARA MACEDO, UFMG; CARLOS RENATO MACHADO, UFMG; TIMOTHY YOSHINO, University of Wisconsin; GLÓRIA REGINA FRANCO, UFMG. |
Título: |
A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 6, p. 707-717, Sept. 2013. |
Idioma: |
Inglês |
Conteúdo: |
Schistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of trans-spliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different libraries. The accessions for each library and for the individual sequences are as follows: (i) adult worms of mixed sexes (LIBEST_027999: JZ139310 - JZ139779), (ii) female adult worms (LIBEST_028000: JZ139780 - JZ140379), (iii) male adult worms (LIBEST_028001: JZ140380 - JZ141002), (iv) eggs (LIBEST_028002: JZ141003 - JZ141497) and (v) schistosomula (LIBEST_028003: JZ141498 - JZ141974). MenosSchistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of trans-spliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different librari... Mostrar Tudo |
Palavras-Chave: |
Interferência de RNA. |
Thesagro: |
Schistosoma Mansoni. |
Thesaurus NAL: |
RNA interference; Trans-splicing. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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