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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
30/10/1995 |
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Data da última atualização: |
17/05/2023 |
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Autoria: |
MONSALUD, M.-J.; MATHEWS, H.; LITZ, R. E.; GRAY, D. J. |
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Afiliação: |
University of Florida. |
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Título: |
Control of hyperhydricity of mango somatic embryos. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v.42, n.2, p.195-206, 1995. |
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ISSN: |
0167-6857 |
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Idioma: |
Inglês |
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Conteúdo: |
Hyperhydricity of immature somatic embryos has been a limiting factor for the development of highly embryogenic suspension cultures of many important mango cultivars. Reversion of hyperhydricity was achieved in two ways: 1) heart-stage somatic embryos (2-3 mm length) were partially dehydrated under controlled conditions at high relative humidity (RH) for 24-48 h and 2) the gelling agent (Gel-Gro) concentration of theplant growth medium was increased from 2.0 to 6.0 g 1-1. Partially dehydrated immature somatic embryos were normal in appearance. Somatic embryos that were partially dehydrated germinated precociously when cultured on maturation medium. Although abscisic acid (ABA) did not reverse hyperhydricity of primary somatic embryos, ABA did stimulate the reversal of this abnormal pattern of development among secondary embryos.ABA (500 mM) inhibited precocious germination and permitted somatic embryo maturation. Partially dehydrated, immature somatic embryos (4-7 mm long) remained viable for up to 32 days in the absence of maturation medium under high RH. |
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Palavras-Chave: |
Abscisc acid; Dehydration. |
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Thesagro: |
Mangifera Indica. |
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Thesaurus Nal: |
somatic embryogenesis; vitrification. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01705naa a2200229 a 4500
001 1634085
005 2023-05-17
008 1995 bl uuuu u00u1 u #d
022 $a0167-6857
100 1 $aMONSALUD, M.-J.
245 $aControl of hyperhydricity of mango somatic embryos.$h[electronic resource]
260 $c1995
520 $aHyperhydricity of immature somatic embryos has been a limiting factor for the development of highly embryogenic suspension cultures of many important mango cultivars. Reversion of hyperhydricity was achieved in two ways: 1) heart-stage somatic embryos (2-3 mm length) were partially dehydrated under controlled conditions at high relative humidity (RH) for 24-48 h and 2) the gelling agent (Gel-Gro) concentration of theplant growth medium was increased from 2.0 to 6.0 g 1-1. Partially dehydrated immature somatic embryos were normal in appearance. Somatic embryos that were partially dehydrated germinated precociously when cultured on maturation medium. Although abscisic acid (ABA) did not reverse hyperhydricity of primary somatic embryos, ABA did stimulate the reversal of this abnormal pattern of development among secondary embryos.ABA (500 mM) inhibited precocious germination and permitted somatic embryo maturation. Partially dehydrated, immature somatic embryos (4-7 mm long) remained viable for up to 32 days in the absence of maturation medium under high RH.
650 $asomatic embryogenesis
650 $avitrification
650 $aMangifera Indica
653 $aAbscisc acid
653 $aDehydration
700 1 $aMATHEWS, H.
700 1 $aLITZ, R. E.
700 1 $aGRAY, D. J.
773 $tPlant Cell, Tissue and Organ Culture$gv.42, n.2, p.195-206, 1995.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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