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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
03/08/2001 |
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Data da última atualização: |
03/08/2001 |
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Autoria: |
LUNA, M. F.; MIGNONE, C. F.; BOLAR DI, J. L. |
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Título: |
The carbon source influences the energetic efficiency of the respiratory chain of N2-fixing Acetobacter diazotrophicus. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 54, p. 564-569, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
Acetobacter diazotrophicus is a diazotrophic bacterium that colonizes sugarcane tissues. Glucose is oxidized to gluconate in the periplasm prior to uptake and metabolism. A membrane-bound glucose dehydrogenase quinoenzyme [ which contains pyrroloquinoline quinone (PQQ) as the prosthetic group] is involved in that oxidation. Gluconate is oxidized further via the hexose monophosphate pathway and tricarboxylic acid cycle. A.diazotrophicus PAL3 was grown in a chemostat with atmospheric nitrogen as the sole N source provided that the dissolved oxygen was maintained at 1.0-2.0% air saturation. The biomass yields of A. diazotrophicus growing with glucose or gluconate with fixed N were very low compared with other heterotrophic bacteria. The biomass yields under N-fixing conditions were more than 30% less than with ammonium as the N source using gluconate as the carbon source but, surprisingly, were only about 14% less with glucose. The following scheme for the metabolism of A. diazotrophicus through the different pathways emerged: (1) the respiratory chain of this organism had a different efficiency of ATP production in the respiratory chain(P:o ratio) under different culture conditions; and (2) N fixation was one (but not the sole) condition under which a higher P:O ratio was observed. The other condition appears to be the expression of an active PQQ-linked glucose dehydrogenase. |
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Palavras-Chave: |
BNF; C; Cana-de-açúcar; Diazotrofico; Diazotrophs; FBN; Fixação biologica de nitrogênio; Sugar Cane. |
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Thesagro: |
Carbono. |
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Thesaurus Nal: |
carbon; nitrogen fixation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02183naa a2200277 a 4500
001 1621237
005 2001-08-03
008 2000 bl --- 0-- u #d
100 1 $aLUNA, M. F.
245 $aThe carbon source influences the energetic efficiency of the respiratory chain of N2-fixing Acetobacter diazotrophicus.
260 $c2000
520 $aAcetobacter diazotrophicus is a diazotrophic bacterium that colonizes sugarcane tissues. Glucose is oxidized to gluconate in the periplasm prior to uptake and metabolism. A membrane-bound glucose dehydrogenase quinoenzyme [ which contains pyrroloquinoline quinone (PQQ) as the prosthetic group] is involved in that oxidation. Gluconate is oxidized further via the hexose monophosphate pathway and tricarboxylic acid cycle. A.diazotrophicus PAL3 was grown in a chemostat with atmospheric nitrogen as the sole N source provided that the dissolved oxygen was maintained at 1.0-2.0% air saturation. The biomass yields of A. diazotrophicus growing with glucose or gluconate with fixed N were very low compared with other heterotrophic bacteria. The biomass yields under N-fixing conditions were more than 30% less than with ammonium as the N source using gluconate as the carbon source but, surprisingly, were only about 14% less with glucose. The following scheme for the metabolism of A. diazotrophicus through the different pathways emerged: (1) the respiratory chain of this organism had a different efficiency of ATP production in the respiratory chain(P:o ratio) under different culture conditions; and (2) N fixation was one (but not the sole) condition under which a higher P:O ratio was observed. The other condition appears to be the expression of an active PQQ-linked glucose dehydrogenase.
650 $acarbon
650 $anitrogen fixation
650 $aCarbono
653 $aBNF
653 $aC
653 $aCana-de-açúcar
653 $aDiazotrofico
653 $aDiazotrophs
653 $aFBN
653 $aFixação biologica de nitrogênio
653 $aSugar Cane
700 1 $aMIGNONE, C. F.
700 1 $aBOLAR DI, J. L.
773 $tApplied and Environmental Microbiology, Washington$gv. 54, p. 564-569, 2000.
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Embrapa Agrobiologia (CNPAB) |
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| 1. |  | DIAS, J. M. C. S.; ROLO, V. C. P.; HONDA, C. S.; SILVA, J. O.; MIGNONE, C. F. Agua de levedura e glicerol como substratos para o crescimento de Bacillus sphaericus. Revista Microbiologia, Sao Paulo, v.22, n.3, p.278, 1991. Suplemento. Resumo| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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