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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/12/2000 |
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Data da última atualização: |
27/12/2000 |
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Autoria: |
REIS, V. M.; REIS JÚNIOR, F. B. dos; SALLES, J. F.; SCHLOTER, M. |
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Título: |
Characterisation of different polyclonal antisera to quantify Herbaspirillum spp. in elephant grass (Pennisetum purpureum Schun.). |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Symbiosis, Rehovot, v. 29, p. 139-150, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
Immunological techniques can be applied to detect micro-organisms in different ecosystems. To follow the fate of Herbaspirillum seropedicae strain Z67 and H . rubrisubalbicans strain M4 in the rhizosphere of elephant grass, Pennisetum purpureum Schun. var. Capim Cana D'Africa, polyclonal antisera were raised against both strains. Both antisera were purified with protein-A, followed by a primary characterization for cross-reactivity. To reduce cross-reactivity for both sera, affinity purification was used for improvement. After that, both sera could be considered as species specific. The detection limit was 105 cells ml-l for the anti H. rubrisubalbicans serum and 1Q6 cells ml-l for the anti H. seropedicae serum. It was shown that two months after inoculation, both strains could be detected in the rhizoplane of elephant grass in high densities. The estimated numbers by ELISA were 107 cells g-1 fresh weight. |
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Palavras-Chave: |
Bactéira diazotrófica; Diazotrophic bacteria; Elephant grass. |
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Thesagro: |
Capim Elefante; Gramínea Forrageira; Pennisetum Purpureum. |
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Thesaurus Nal: |
forage grasses; Herbaspirillum. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01690naa a2200253 a 4500
001 1597264
005 2000-12-27
008 2000 bl --- 0-- u #d
100 1 $aREIS, V. M.
245 $aCharacterisation of different polyclonal antisera to quantify Herbaspirillum spp. in elephant grass (Pennisetum purpureum Schun.).
260 $c2000
520 $aImmunological techniques can be applied to detect micro-organisms in different ecosystems. To follow the fate of Herbaspirillum seropedicae strain Z67 and H . rubrisubalbicans strain M4 in the rhizosphere of elephant grass, Pennisetum purpureum Schun. var. Capim Cana D'Africa, polyclonal antisera were raised against both strains. Both antisera were purified with protein-A, followed by a primary characterization for cross-reactivity. To reduce cross-reactivity for both sera, affinity purification was used for improvement. After that, both sera could be considered as species specific. The detection limit was 105 cells ml-l for the anti H. rubrisubalbicans serum and 1Q6 cells ml-l for the anti H. seropedicae serum. It was shown that two months after inoculation, both strains could be detected in the rhizoplane of elephant grass in high densities. The estimated numbers by ELISA were 107 cells g-1 fresh weight.
650 $aforage grasses
650 $aHerbaspirillum
650 $aCapim Elefante
650 $aGramínea Forrageira
650 $aPennisetum Purpureum
653 $aBactéira diazotrófica
653 $aDiazotrophic bacteria
653 $aElephant grass
700 1 $aREIS JÚNIOR, F. B. dos
700 1 $aSALLES, J. F.
700 1 $aSCHLOTER, M.
773 $tSymbiosis, Rehovot$gv. 29, p. 139-150, 2000.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 8 | |
| 2. |  | FERREIRA, P. S.; FABRICIO, A. C.; SILVA, V. B.; SALTON, J. C.; SCHLOTER, M.; LANGENBACH, T. Influência de diferentes manejos agrícolas na diversidade bacteriana e na produtividade em agroecossistema tropical. In: CONGRESSO BRASILEIRO DE CIENCIA DO SOLO, 28., 2001, Londrina. Ciência do solo: fator de produtividade competitiva com sustentabilidade. Londrina: Sociedade Brasileira d Ciência do Solo, 2001. Londrina: Sociedade Brasileira de Ciência do Solo, 2001. p. 97| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agropecuária Oeste. |
|    |
| 3. | | FERREIRA, P. S.; FABRÍCIO, A. C.; SILVA, V. B.; SCHLOTER, M.; LANGENBACH, T. Influence of crop rotation on microbial activity and yield in Cerrado agroecosystem. In: INTERNATIONAL CONFERENCE ON LAND DEGRADATION , 3.; MEETING OFTHE IUSS SUBCOMMISSION C-SOIL AND WATER CONSERVATION, 3., 2001, Rio de Janeiro. Land degradation: new trends towards global sustainability: conference guide, program and book abstracts. [Rio de Janeiro: Embrapa Solos, 2001?]. p.115.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 4. | | ROUWS, L. F. M.; FISCHER, D.; ARAUJO, J. L. S. de; SCHMID, M.; SCHLOTER, M.; REIS, V. M.; BALDANI, J. I.; HARTMANN, A. Bradyrhizobium spp. are part of the nitrogen-fixing bacterial community associated with the field grown sugar cane Seropédica/ Rio de Janeiro, Brasil. EUROPEAN NITROGEN FIXATION CONFERENCE, 10., 2012, Munich. Abstract.... Munich: German Research Center for Environmental Health, 2012. 72 p. PP3-30| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 5. | | LEITE, J.; FISCHER, D.; ROUWS, L. F. M.; FERNANDES JUNIOR, P. I.; HOFMANN, A.; KUBLIK, S.; SCHLOTER, M.; XAVIER, G. R.; RADL, V. Cowpea nodules harbor non-rhizobial bacterial communities that are shaped by soil type rather than plant genotype. Frontiers in Plant Science, v. 7, p. 1-11, jan. 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Semiárido. |
|  |
| 6. | | LEITE, J.; FISCHER, D.; ROUWS, L. F. M.; FERNANDES JUNIOR, P. I.; HOFMANN, A.; KUBLIK, S.; SCHLOTER, M.; XAVIER, G. R.; RADL, V. Cowpea nodules harbor non-rhizobial bacterial communities that are shaped by soil type rather than plant genotype. Frontiers in Plant Science v. 7, p. 1-11, jan. 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 7. | | FISCHER, D.; PFITZNER, B.; SCHIMID, M.; SIMÕES-ARAUJO J. L.; REIS, V. M.; PEREIRA, W.; ORMEÑO-ORRILHO, E.; HAI, B.; HOFMANN, A.; SCHLOTER, M.; MARTINEZ-ROMERO, E.; BALDANI, J. I.; HARTMANN, A. Molecular characterisaton of the dizotrophic bacterial community in uninoculated and inoculated field-grown sugarcane (Saccharum sp.) Plant and Soil Online, 27 may 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 8. | | FISCHER, D.; PFITZNER, B.; SCHMID, M; ARAUJO, J. L. S. de; REIS, V. M.; PEREIRA, W.; ORMEÑO-ORRILO; HAI, B.; HOFMANN, A.; SCHLOTER, M.; MARTINEZ-ROMERO, E.; BALDANI, J. I.; HARTMANN, A. Molecular characterization of the diazotrophic bacterial community in uninoculated and inoculate field-grown sugarcane (Saccharum sp.). Plant and Soil, v. 356, p. 83-99, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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