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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
25/03/2003 |
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Data da última atualização: |
25/03/2003 |
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Autoria: |
PROBERT, M. E.; KEATING, B. A.; LARKENS, A. G.; SIAMBI, M. M. |
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Título: |
Regional assessment of strategies for maize production in semi-arid eastern Kenya. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Indooroopilly: CSIRO, 2001. |
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Páginas: |
34 p. |
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Série: |
(Tropical Agriculture Technical Memorandum, 8). |
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ISBN: |
0643064664 |
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Idioma: |
Português |
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Conteúdo: |
This report arises from extension fo ACIAR Project nº 8735 " Improvement of dryland crop and forage production in semi-arid Africa". It extends earlier studies of strategies for improving maize production in semi-arid eastern Kenya to the wider geographical region of Machakos / Kitui Districts.
Climatic data were collated for 44 sites in the region. Seven of these sites were selected, representing the range of agroecological zones in the disctrict and for which a good length of daily raingall records are available. These were used as input for the maize model CMKEN to explore a number of management options that impinge on maize yields and riskiness of cropping. For most of the scenarios simulated, two soils were used; these differed mainly in respect of their water holding characteristics... |
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Palavras-Chave: |
Kenia; Semi-arido. |
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Thesagro: |
Milho. |
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Thesaurus Nal: |
Africa. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01415nam a2200229 a 4500
001 1566638
005 2003-03-25
008 2001 bl uuuu u0uu1 u #d
020 $a0643064664
100 1 $aPROBERT, M. E.
245 $aRegional assessment of strategies for maize production in semi-arid eastern Kenya.
260 $aIndooroopilly: CSIRO$c2001
300 $a34 p.
490 $a(Tropical Agriculture Technical Memorandum, 8).
520 $aThis report arises from extension fo ACIAR Project nº 8735 " Improvement of dryland crop and forage production in semi-arid Africa". It extends earlier studies of strategies for improving maize production in semi-arid eastern Kenya to the wider geographical region of Machakos / Kitui Districts. Climatic data were collated for 44 sites in the region. Seven of these sites were selected, representing the range of agroecological zones in the disctrict and for which a good length of daily raingall records are available. These were used as input for the maize model CMKEN to explore a number of management options that impinge on maize yields and riskiness of cropping. For most of the scenarios simulated, two soils were used; these differed mainly in respect of their water holding characteristics...
650 $aAfrica
650 $aMilho
653 $aKenia
653 $aSemi-arido
700 1 $aKEATING, B. A.
700 1 $aLARKENS, A. G.
700 1 $aSIAMBI, M. M.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
06/12/2011 |
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Data da última atualização: |
24/01/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MOURÃO, M. M.; BITAR, M. L.; LOBO, F. P.; GRYNBERG, P.; BORONI, M.; WAISBERG, M.; MACEDO, A. M.; MACHADO, C. R.; FRANCO, G. R. |
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Afiliação: |
MARINA MORAES MOURÃO, FIOCRUZ; MAINÁ LAST BITAR, UFMG, NUBIO; FRANCISCO PEREIRA LOBO, CNPTIA; PRISCILA GRYNBERG, UFMG; MARIANA BORONI, UFMG; MICHAEL WAISBERG, NIH; ANDRÉA MARA MACEDO, UFMG; CARLOS RENATO MACHADO, UFMG; GLÓRIA REGINA FRANCO, UFMG. |
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Título: |
Revealing new perspectives on the regulation of gene expression by the trans-splicing mechanism in Schistosoma mansoni. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 7.; INTERNATIONAL CONFERENCE OF THE IBEROAMERICAN SOCIETY FOR BIOINFORMATICS, 3., 2011, Florianópolis. Proceedings... Florianópolis: Associação Brasileira de Bioinformática e Biologia Computacional, 2011. |
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Páginas: |
Não paginado. |
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Idioma: |
Inglês |
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Notas: |
X-MEETING 2011. |
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Conteúdo: |
Schistosomiasis is the second most prevalent neglected tropical disease caused by a Trematode flatworm from the genus Schistosoma. Schistosomes possess a complex life cycle requiring numerous and intricate mechanisms to rule transcriptional and post-transcriptional gene regulation. Thus, it is believed that spliced leader (SL) trans-splicing could play an important role in the parasite biology. The purpose of this study was is to shed light on the function of the trans-splicing mechanism in S. mansoni by searching gene categories that could be target of this process and attempting to silence transcripts harboring the SL sequence. Here, we analyze different life stages cDNA libraries enriched on SL-transcripts of S. mansoni. Our results show that, contrary to previous hypothesis, trans-spliced transcripts are associated to specific gene categories, such as carbohydrate metabolic process, RNA metabolism and DNA repair. Several trans-spliced transcripts were verified in at least two different stages of the S. mansoni life cycle. The marjority of the SL-transcripts are shared among males, females and mixed-sex adults, being followed by SL-transcripts shared by all adults and schistosomula. Among the analyzed life stages, the one presenting the lower number of shared genes is the egg. The results also reveal a set of SL-transcripts that are observed only in one stage of the life cycle and could represent transcripts that undergo stage-specific trans-splicing. Finally, we demonstrated that trans-splicing knockdown in sprocysts caused a reduction on the levels of all tested trans-spliced transcripts, but surprisingly the only phenotypic effect observed was a diminished larvae size. Financial support: CNPq, FAPEMIG MenosSchistosomiasis is the second most prevalent neglected tropical disease caused by a Trematode flatworm from the genus Schistosoma. Schistosomes possess a complex life cycle requiring numerous and intricate mechanisms to rule transcriptional and post-transcriptional gene regulation. Thus, it is believed that spliced leader (SL) trans-splicing could play an important role in the parasite biology. The purpose of this study was is to shed light on the function of the trans-splicing mechanism in S. mansoni by searching gene categories that could be target of this process and attempting to silence transcripts harboring the SL sequence. Here, we analyze different life stages cDNA libraries enriched on SL-transcripts of S. mansoni. Our results show that, contrary to previous hypothesis, trans-spliced transcripts are associated to specific gene categories, such as carbohydrate metabolic process, RNA metabolism and DNA repair. Several trans-spliced transcripts were verified in at least two different stages of the S. mansoni life cycle. The marjority of the SL-transcripts are shared among males, females and mixed-sex adults, being followed by SL-transcripts shared by all adults and schistosomula. Among the analyzed life stages, the one presenting the lower number of shared genes is the egg. The results also reveal a set of SL-transcripts that are observed only in one stage of the life cycle and could represent transcripts that undergo stage-specific trans-splicing. Finally, we demonstr... Mostrar Tudo |
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Palavras-Chave: |
Expressão gênica. |
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Thesagro: |
Schistosoma Mansoni. |
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Thesaurus NAL: |
Gene expression. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/908752/1/Shistosoma.pdf
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Marc: |
LEADER 02793nam a2200265 a 4500
001 1908752
005 2020-01-24
008 2011 bl uuuu u00u1 u #d
100 1 $aMOURÃO, M. M.
245 $aRevealing new perspectives on the regulation of gene expression by the trans-splicing mechanism in Schistosoma mansoni.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 7.; INTERNATIONAL CONFERENCE OF THE IBEROAMERICAN SOCIETY FOR BIOINFORMATICS, 3., 2011, Florianópolis. Proceedings... Florianópolis: Associação Brasileira de Bioinformática e Biologia Computacional$c2011
300 $aNão paginado.
500 $aX-MEETING 2011.
520 $aSchistosomiasis is the second most prevalent neglected tropical disease caused by a Trematode flatworm from the genus Schistosoma. Schistosomes possess a complex life cycle requiring numerous and intricate mechanisms to rule transcriptional and post-transcriptional gene regulation. Thus, it is believed that spliced leader (SL) trans-splicing could play an important role in the parasite biology. The purpose of this study was is to shed light on the function of the trans-splicing mechanism in S. mansoni by searching gene categories that could be target of this process and attempting to silence transcripts harboring the SL sequence. Here, we analyze different life stages cDNA libraries enriched on SL-transcripts of S. mansoni. Our results show that, contrary to previous hypothesis, trans-spliced transcripts are associated to specific gene categories, such as carbohydrate metabolic process, RNA metabolism and DNA repair. Several trans-spliced transcripts were verified in at least two different stages of the S. mansoni life cycle. The marjority of the SL-transcripts are shared among males, females and mixed-sex adults, being followed by SL-transcripts shared by all adults and schistosomula. Among the analyzed life stages, the one presenting the lower number of shared genes is the egg. The results also reveal a set of SL-transcripts that are observed only in one stage of the life cycle and could represent transcripts that undergo stage-specific trans-splicing. Finally, we demonstrated that trans-splicing knockdown in sprocysts caused a reduction on the levels of all tested trans-spliced transcripts, but surprisingly the only phenotypic effect observed was a diminished larvae size. Financial support: CNPq, FAPEMIG
650 $aGene expression
650 $aSchistosoma Mansoni
653 $aExpressão gênica
700 1 $aBITAR, M. L.
700 1 $aLOBO, F. P.
700 1 $aGRYNBERG, P.
700 1 $aBORONI, M.
700 1 $aWAISBERG, M.
700 1 $aMACEDO, A. M.
700 1 $aMACHADO, C. R.
700 1 $aFRANCO, G. R.
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