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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
17/09/2007 |
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Data da última atualização: |
17/06/2025 |
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Autoria: |
BWANGA, C. O.; BRAGANÇA, M; M. de; EINARSSON, S.; RODRIGUEZ-MARTINEZ, H. |
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Título: |
Cryopreservation of boar semen in mini- and maxi-straws. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Journal or Veterinary Medicine, v. 37, n. 9, p. 691-658, 1990. |
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DOI: |
10.1111/j.1439-0442.1990.tb00958.x |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Split ejaculates from four boars were frozen with a programmable freezing machine, in mini- (0.25 ml) and maxi- (5 ml) plastic straws with an extender at either acidic (6.3) or alkaline (7.4) pH. Glycerol (3%) was used as cryoprotectant. The freezing of the semen was monitored by way of thermocouples placed in the straws. Post-thaw motility and acrosome integrity were evaluated; the latter using phase contrast microscopy, eosin-nigrosin stain and electron microscopy. Post-thaw sperm motility was significantly higher when semen was frozen in mini-straws than in maxi-straws. For the mini-straws, the motility was better when semen was exposed to an acidic environment during freezing, but this beneficial effect of the low extracellular pH was not evident when maxi-straws were thawed. The motility of the spermatozoa diminished significantly during the thermoresistance test (0 h and 2 h time) at 37 degrees C in a similar way for both straws and extracellular pH's. The freezing procedure, no matter the extracellular pH, did not cause major acrosomal damages, but significantly more normal apical ridges were present in the mini-straws than in the maxi-straws. This in vitro evaluation indicated that the freezing method employed was better for mini- than for maxi-straws since the freezing of the 5 ml volumes was not homogeneous, due to the large section area between the surface and the core of the straw. |
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Palavras-Chave: |
In vitro; Palheta; Semen Preservation. |
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Thesagro: |
Congelamento; Inseminação Artificial; Sêmen; Suíno. |
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Thesaurus Nal: |
Artificial insemination; Males; Sperm motility; Swine. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02282naa a2200313 a 4500
001 1533328
005 2025-06-17
008 1990 bl uuuu u00u1 u #d
024 7 $a10.1111/j.1439-0442.1990.tb00958.x$2DOI
100 1 $aBWANGA, C. O.
245 $aCryopreservation of boar semen in mini- and maxi-straws.$h[electronic resource]
260 $c1990
520 $aAbstract: Split ejaculates from four boars were frozen with a programmable freezing machine, in mini- (0.25 ml) and maxi- (5 ml) plastic straws with an extender at either acidic (6.3) or alkaline (7.4) pH. Glycerol (3%) was used as cryoprotectant. The freezing of the semen was monitored by way of thermocouples placed in the straws. Post-thaw motility and acrosome integrity were evaluated; the latter using phase contrast microscopy, eosin-nigrosin stain and electron microscopy. Post-thaw sperm motility was significantly higher when semen was frozen in mini-straws than in maxi-straws. For the mini-straws, the motility was better when semen was exposed to an acidic environment during freezing, but this beneficial effect of the low extracellular pH was not evident when maxi-straws were thawed. The motility of the spermatozoa diminished significantly during the thermoresistance test (0 h and 2 h time) at 37 degrees C in a similar way for both straws and extracellular pH's. The freezing procedure, no matter the extracellular pH, did not cause major acrosomal damages, but significantly more normal apical ridges were present in the mini-straws than in the maxi-straws. This in vitro evaluation indicated that the freezing method employed was better for mini- than for maxi-straws since the freezing of the 5 ml volumes was not homogeneous, due to the large section area between the surface and the core of the straw.
650 $aArtificial insemination
650 $aMales
650 $aSperm motility
650 $aSwine
650 $aCongelamento
650 $aInseminação Artificial
650 $aSêmen
650 $aSuíno
653 $aIn vitro
653 $aPalheta
653 $aSemen Preservation
700 1 $aBRAGANÇA, M
700 1 $aM. de
700 1 $aEINARSSON, S.
700 1 $aRODRIGUEZ-MARTINEZ, H.
773 $tJournal or Veterinary Medicine$gv. 37, n. 9, p. 691-658, 1990.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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