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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
05/06/2003 |
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Data da última atualização: |
25/08/2023 |
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Autoria: |
AVENA, M.; BERGDOLL, M. S. |
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Título: |
Purification and some physicochemical properties of enterotoxin C, Staphylococcus aureus strain 361. |
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Ano de publicação: |
1967 |
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Fonte/Imprenta: |
Biochemistry, v. 6, n. 5, p. 1474-1480, May, 1967. |
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Idioma: |
Inglês |
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Conteúdo: |
Enterotoxin C elaborated by Staphylococcus aureus strain 361 has been purified by ion-exchange chromatography on CM-cellulose (stepwise and gradient elution) and molecular sieving through Sephadex G-75 and G-50 gels. Several lines of evidence are presented to demonstrate that the purified enterotoxin possesses a high degree of homogeneity. The highly purified antigenic preparation is a simple protein which bears significant emetic activity. Some of the determined physicochemical properties are sedimentation coefficient, 2.9 S; molecular weight, about 34,000; frictional ratio, 1.19; and isoelectric pH, approximately 7.0 in sodium phosphate buffer of 0.1 ionic strength. This neutral isoelectric point indicates that enterotoxin C produced by strain 361 is chemically distinct from an immunologically similar one isolated from strain 137. |
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Palavras-Chave: |
Bacteriologia veterinária; Enterotoxina; Estafilococos; Spectrophotometry. |
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Thesagro: |
Enterotoxemia; Imunidade; Propriedade Físico-Química; Purificação; Staphylococcus Aureus. |
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Thesaurus Nal: |
Antigen-antibody reactions; Centrifugation; Electrophoresis; Enterotoxins; Immunodiffusion tests; Ion exchange chromatography; Pharmacology; Toxins; Urea. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01864naa a2200349 a 4500
001 1529401
005 2023-08-25
008 1967 bl uuuu u00u1 u #d
100 1 $aAVENA, M.
245 $aPurification and some physicochemical properties of enterotoxin C, Staphylococcus aureus strain 361.$h[electronic resource]
260 $c1967
520 $aEnterotoxin C elaborated by Staphylococcus aureus strain 361 has been purified by ion-exchange chromatography on CM-cellulose (stepwise and gradient elution) and molecular sieving through Sephadex G-75 and G-50 gels. Several lines of evidence are presented to demonstrate that the purified enterotoxin possesses a high degree of homogeneity. The highly purified antigenic preparation is a simple protein which bears significant emetic activity. Some of the determined physicochemical properties are sedimentation coefficient, 2.9 S; molecular weight, about 34,000; frictional ratio, 1.19; and isoelectric pH, approximately 7.0 in sodium phosphate buffer of 0.1 ionic strength. This neutral isoelectric point indicates that enterotoxin C produced by strain 361 is chemically distinct from an immunologically similar one isolated from strain 137.
650 $aAntigen-antibody reactions
650 $aCentrifugation
650 $aElectrophoresis
650 $aEnterotoxins
650 $aImmunodiffusion tests
650 $aIon exchange chromatography
650 $aPharmacology
650 $aToxins
650 $aUrea
650 $aEnterotoxemia
650 $aImunidade
650 $aPropriedade Físico-Química
650 $aPurificação
650 $aStaphylococcus Aureus
653 $aBacteriologia veterinária
653 $aEnterotoxina
653 $aEstafilococos
653 $aSpectrophotometry
700 1 $aBERGDOLL, M. S.
773 $tBiochemistry$gv. 6, n. 5, p. 1474-1480, May, 1967.
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| 1. |  | AMARAL, G. A.; EUCLIDES, V. P. B.; NANTES, N. N.; MONTAGNER, D. B.; ECHEVERRIA, J. R.; ANTUNES, L. E.; CANHETE, A. F. Produtividade e desempenho animal em pastos de Brachiaria brizantha cv. Marandu e híbrido de Brachiaria H331, sob lotação intermitente. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 9., 2013, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2013. (Embrapa Gado de Corte. Documentos, 204). Comissão organizadora: Denise Baptaglin Montagner, Grácia Maria Soares Rosinha, Rodrigo Carvalho Alva.| Tipo: Resumo em Anais de Congresso |
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