| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
28/05/2003 |
|
Data da última atualização: |
10/01/2024 |
|
Autoria: |
GOLDSMIT, L.; BARZILAI, E. |
|
Título: |
An improved method for the isolation and identification of bluetongue virus by intravenous inoculation of embryonated chicken eggs. |
|
Ano de publicação: |
1968 |
|
Fonte/Imprenta: |
Journal of Comparative Pathology, v. 78, n. 4, p. 477-487, Oct. 1968. |
|
DOI: |
https://doi.org/10.1016/0021-9975(68)90047-9 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: Twenty-eight samples from suspected field cases of bluetongue were titrated simultaneously in embryonating chicken eggs by the intravenous and by the yolk sac routes of inoculation. Type 16 virus was isolated from all the 28 samples inoculated intravenously. When they were inoculated into the yolk sac 19 samples tested were positive and 9 were negative. The highest titre of the virus in the intravenously inoculated eggs was 105·0 chicken embryo LD50 per 0·1 ml. The highest titre in the yolk sac was . Those samples which had a titre of or lower after intravenous inoculation were negative by the yolk sac method. When the first egg passages were titrated simultaneously by the intravenous and yolk sac routes the difference between the LD50 titres of the virus in the intravenous and yolk sac inoculated eggs respectively was 3 logs. Embryo mortality following the intravenous inoculation was one hundred per cent. in the first egg passage. The time required for isolation and typing of the virus when this route was used was 10 days. When the yolk sac was used the mortality did not reach one hundred per cent. until the sixth or seventh consecutive egg passage. The time required for isolation and typing of the virus when this route was employed was 7 to 8 weeks. |
|
Palavras-Chave: |
Isolamento viral. |
|
Thesagro: |
Doença Animal; Língua Azul; Vírus. |
|
Thesaurus Nal: |
Assays; Bluetongue; Cattle diseases; Culicoides; Sheep diseases. |
|
Categoria do assunto: |
H Saúde e Patologia |
|
Marc: |
LEADER 02080naa a2200253 a 4500
001 1529354
005 2024-01-10
008 1968 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/0021-9975(68)90047-9$2DOI
100 1 $aGOLDSMIT, L.
245 $aAn improved method for the isolation and identification of bluetongue virus by intravenous inoculation of embryonated chicken eggs.$h[electronic resource]
260 $c1968
520 $aAbstract: Twenty-eight samples from suspected field cases of bluetongue were titrated simultaneously in embryonating chicken eggs by the intravenous and by the yolk sac routes of inoculation. Type 16 virus was isolated from all the 28 samples inoculated intravenously. When they were inoculated into the yolk sac 19 samples tested were positive and 9 were negative. The highest titre of the virus in the intravenously inoculated eggs was 105·0 chicken embryo LD50 per 0·1 ml. The highest titre in the yolk sac was . Those samples which had a titre of or lower after intravenous inoculation were negative by the yolk sac method. When the first egg passages were titrated simultaneously by the intravenous and yolk sac routes the difference between the LD50 titres of the virus in the intravenous and yolk sac inoculated eggs respectively was 3 logs. Embryo mortality following the intravenous inoculation was one hundred per cent. in the first egg passage. The time required for isolation and typing of the virus when this route was used was 10 days. When the yolk sac was used the mortality did not reach one hundred per cent. until the sixth or seventh consecutive egg passage. The time required for isolation and typing of the virus when this route was employed was 7 to 8 weeks.
650 $aAssays
650 $aBluetongue
650 $aCattle diseases
650 $aCulicoides
650 $aSheep diseases
650 $aDoença Animal
650 $aLíngua Azul
650 $aVírus
653 $aIsolamento viral
700 1 $aBARZILAI, E.
773 $tJournal of Comparative Pathology$gv. 78, n. 4, p. 477-487, Oct. 1968.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 2 | |
| 1. |  | VIEGAS, M. P.; SILVA, C. L. S. P.; PAULA, D. R.; MENDES, H. S. J.; ANA YAMAGUISHI CIAMPI; AZEVEDO, V. C. R.; SEBBENN, A. M.; FREITAS, M. L. M.; MORAES, M. L. T. Diversidade genética em população de Myracrodruon urundeuva (Fr. All.) sob perturbação antrópica. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 237.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|    |
| 2. | | SILVA, C. L. S. P.; VIEGAS, M. P.; PAULA, D. R.; MENDES, H. J.; CIAMPI, A. Y.; AZEVEDO, V. C. R.; SEBBENN, A. M.; FREITAS, M. L. M.; MORAES, M. L. T. "Pool" alélico de duas populações de Myracrodruon urundeuva (Fr. All.) sob ação antrópica. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 193.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| Registros recuperados : 2 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
