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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
23/05/2000 |
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Data da última atualização: |
03/08/2023 |
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Autoria: |
SIMMONS, C. P.; DUNSTAN, S. J.; TACHEDJIAN, M.; KRYWULT, J.; HODSON, A. L. M.; STRUGNELL, R. A. |
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Título: |
Vaccine potential of attenuated mutants of Corynebacterium pseudotuberculosis in sheep. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Infection and Immunity, v. 66, n. 2, p. 474-479, Feb. 1998. |
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DOI: |
10.1128/IAI.66.2.474-479.1998. |
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Idioma: |
Inglês |
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Conteúdo: |
Corynebacterium pseudotuberculosis, a gram-positive facultative intracellular bacterial pathogen, is the etiological agent of the economically important disease caseous lymphadenitis (CLA) in both sheep and goats. Attenuated mutants of C. pseudotuberculosis have the potential to act as novel vaccines against CLA and as veterinary vaccine vectors. In this report, we have assessed the virulence of both aroQ and pldmutants of C. pseudotuberculosis in sheep and concurrently their capacity to act as vaccines against homologous challenge. The results suggest that aroQ mutants of C. pseudotuberculosis are attenuated with regard to both lymph node persistence and vaccination site reactogenicity. Immunologically,aroQ mutants failed to elicit detectable specific gamma interferon (IFN-?)-secreting lymphocytes and induced low levels of antibodies to C. pseudotuberculosis culture supernatant antigens. Following subcutaneous vaccination, the immune responses induced by aroQ mutants did not protect sheep from infection with the wild-type strain but did appear to reduce the clinical severity of disease resulting from challenge. Conversely, an attenuated C. pseudotuberculosis strain expressing an enzymatically inactive phospholipase D exotoxin, when used as a vaccine, elicited a protective immune response. Protection appeared to correlate with in vivo persistence of the vaccine strain, the induction of IFN-?-secreting lymphocytes, and relatively high levels of antibodies to culture supernatant antigens. The results suggest thataroQ mutants of C. pseudotuberculosis may be overly attenuated for use as a CLA vaccines or as vaccine vectors. MenosCorynebacterium pseudotuberculosis, a gram-positive facultative intracellular bacterial pathogen, is the etiological agent of the economically important disease caseous lymphadenitis (CLA) in both sheep and goats. Attenuated mutants of C. pseudotuberculosis have the potential to act as novel vaccines against CLA and as veterinary vaccine vectors. In this report, we have assessed the virulence of both aroQ and pldmutants of C. pseudotuberculosis in sheep and concurrently their capacity to act as vaccines against homologous challenge. The results suggest that aroQ mutants of C. pseudotuberculosis are attenuated with regard to both lymph node persistence and vaccination site reactogenicity. Immunologically,aroQ mutants failed to elicit detectable specific gamma interferon (IFN-?)-secreting lymphocytes and induced low levels of antibodies to C. pseudotuberculosis culture supernatant antigens. Following subcutaneous vaccination, the immune responses induced by aroQ mutants did not protect sheep from infection with the wild-type strain but did appear to reduce the clinical severity of disease resulting from challenge. Conversely, an attenuated C. pseudotuberculosis strain expressing an enzymatically inactive phospholipase D exotoxin, when used as a vaccine, elicited a protective immune response. Protection appeared to correlate with in vivo persistence of the vaccine strain, the induction of IFN-?-secreting lymphocytes, and relatively high levels of antibodies to culture supernata... Mostrar Tudo |
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Palavras-Chave: |
Attenuated vaccines. |
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Thesagro: |
Linfadenite Caseosa; Ovino; Vacina. |
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Thesaurus Nal: |
Bacterial antibodies; Bacterial vaccines; Corynebacterium; Hydro-lyases; Immunoglobulin G; Immunology; Live vaccines; Mutation; Sheep diseases; Vaccination. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02673naa a2200361 a 4500
001 1524118
005 2023-08-03
008 1998 bl uuuu u00u1 u #d
024 7 $a10.1128/IAI.66.2.474-479.1998.$2DOI
100 1 $aSIMMONS, C. P.
245 $aVaccine potential of attenuated mutants of Corynebacterium pseudotuberculosis in sheep.$h[electronic resource]
260 $c1998
520 $aCorynebacterium pseudotuberculosis, a gram-positive facultative intracellular bacterial pathogen, is the etiological agent of the economically important disease caseous lymphadenitis (CLA) in both sheep and goats. Attenuated mutants of C. pseudotuberculosis have the potential to act as novel vaccines against CLA and as veterinary vaccine vectors. In this report, we have assessed the virulence of both aroQ and pldmutants of C. pseudotuberculosis in sheep and concurrently their capacity to act as vaccines against homologous challenge. The results suggest that aroQ mutants of C. pseudotuberculosis are attenuated with regard to both lymph node persistence and vaccination site reactogenicity. Immunologically,aroQ mutants failed to elicit detectable specific gamma interferon (IFN-?)-secreting lymphocytes and induced low levels of antibodies to C. pseudotuberculosis culture supernatant antigens. Following subcutaneous vaccination, the immune responses induced by aroQ mutants did not protect sheep from infection with the wild-type strain but did appear to reduce the clinical severity of disease resulting from challenge. Conversely, an attenuated C. pseudotuberculosis strain expressing an enzymatically inactive phospholipase D exotoxin, when used as a vaccine, elicited a protective immune response. Protection appeared to correlate with in vivo persistence of the vaccine strain, the induction of IFN-?-secreting lymphocytes, and relatively high levels of antibodies to culture supernatant antigens. The results suggest thataroQ mutants of C. pseudotuberculosis may be overly attenuated for use as a CLA vaccines or as vaccine vectors.
650 $aBacterial antibodies
650 $aBacterial vaccines
650 $aCorynebacterium
650 $aHydro-lyases
650 $aImmunoglobulin G
650 $aImmunology
650 $aLive vaccines
650 $aMutation
650 $aSheep diseases
650 $aVaccination
650 $aLinfadenite Caseosa
650 $aOvino
650 $aVacina
653 $aAttenuated vaccines
700 1 $aDUNSTAN, S. J.
700 1 $aTACHEDJIAN, M.
700 1 $aKRYWULT, J.
700 1 $aHODSON, A. L. M.
700 1 $aSTRUGNELL, R. A.
773 $tInfection and Immunity$gv. 66, n. 2, p. 474-479, Feb. 1998.
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