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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
21/03/1996 |
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Data da última atualização: |
30/06/2023 |
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Autoria: |
FISER, P. S.; MARCUS, G. J. |
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Título: |
Continuous live-dead discrimination of ram sperm during freezing. |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
Gamede Research, v. 22, n. 3, p. 301-305, Mar. 1989. |
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DOI: |
10.1002/mrd.1120220307. |
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Idioma: |
Inglês |
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Conteúdo: |
Use of the dye amarabth (Color Index 16185) as a supravital stain for ram sperm is described. At a concentration of 0,4% in diluted semen, the dye was completely excluded by motile sperm and had no effect on sperm motility. The nuclei of immotile sperm were stained pink by amaranth. The decrease in sperm motility during 24-h storage at 5gC was accompained by a corresponding increase in stained sperm nuclei. The presence of the dye during freezing had no effect on sperm cryosurvival but tended to reduce sperm motility during post-thaw incubation. Insemination of ewes with fresch semen containing amaranth or with semen frozen in the presence of amaranth resulted in pregnancies in 7/10 ewes in each group, compared to 6/9 in the case of ewes inseminated with freshsemen without dye. |
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Palavras-Chave: |
Coloracao vital; Congelacao. |
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Thesagro: |
Ovino; Reprodução; Sêmen. |
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Thesaurus Nal: |
Amaranth dye; Animal fertility; Freezing; Pharmacology; Rams; Reproduction; Sheep; Sperm motility; Spermatozoa. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01606naa a2200313 a 4500
001 1523584
005 2023-06-30
008 1989 bl uuuu u00u1 u #d
024 7 $a10.1002/mrd.1120220307.$2DOI
100 1 $aFISER, P. S.
245 $aContinuous live-dead discrimination of ram sperm during freezing.$h[electronic resource]
260 $c1989
520 $aUse of the dye amarabth (Color Index 16185) as a supravital stain for ram sperm is described. At a concentration of 0,4% in diluted semen, the dye was completely excluded by motile sperm and had no effect on sperm motility. The nuclei of immotile sperm were stained pink by amaranth. The decrease in sperm motility during 24-h storage at 5gC was accompained by a corresponding increase in stained sperm nuclei. The presence of the dye during freezing had no effect on sperm cryosurvival but tended to reduce sperm motility during post-thaw incubation. Insemination of ewes with fresch semen containing amaranth or with semen frozen in the presence of amaranth resulted in pregnancies in 7/10 ewes in each group, compared to 6/9 in the case of ewes inseminated with freshsemen without dye.
650 $aAmaranth dye
650 $aAnimal fertility
650 $aFreezing
650 $aPharmacology
650 $aRams
650 $aReproduction
650 $aSheep
650 $aSperm motility
650 $aSpermatozoa
650 $aOvino
650 $aReprodução
650 $aSêmen
653 $aColoracao vital
653 $aCongelacao
700 1 $aMARCUS, G. J.
773 $tGamede Research$gv. 22, n. 3, p. 301-305, Mar. 1989.
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Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 6 | |
| 2. |  | SILVA, A. E. D. F. da; DODE, M. A.; PORTO, J. C. A.; ABREU, U. G. P. de. Estacionalidade na atividade sexual de machos bovinos Nelore e mestiços Fleckvieh e Chianina x Nelore: características espermáticas. Pesquisa Agropecuária Brasileira, Brasília, DF, v.26, n.10, p.1751-1760, out. 1991.| Biblioteca(s): Embrapa Pantanal. |
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| 4. | | SERENO, J. R. B.; SERENO, F. T. P. S.; MORENO, T.; DODE, M. A. N.; CABELLO, A. Dificultades en la extracion y congelacion de semen de animales domesticos inseridos en programas de conservacion en sus proprios habitats. In: SIMPOSIO IBEROAMERICANO SOBRE CONSERVACION DE LOS RECURSOS GENETICOS LOCALES Y EL DESARROLLO RURAL SUSTENTABLE, 2., 2001, Coro. Programa y resumenes. Coro, Falcon: Fundacite Falcon, 2001. p.65.| Biblioteca(s): Embrapa Pantanal. |
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| 5. | | SERENO, J. R. B.; DELGADO, J. V.; SERENO, F. T. P. de S.; BARBA, C.; CABELLO, A.; DODE, M. A. N. Conservacao ex situ de suinos ibericos atraves da criopreservacao de semen. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 39., 2002, Recife. A producao animal e a sociedade brasileira. Anais. Recife: UFRPE / SBZ, 2002. CD-ROM. (Secao Manejo e Reproducao) nao paginado.| Biblioteca(s): Embrapa Pantanal. |
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| 6. | | NOGUEIRA, E.; SANCHES, C.; COSTA E SILVA, E. V.; MENDES, A.; DODE, M. A. N.; WILEY, G.; KERNS, K.; SUTOVSKY, P. Identification of seminal parameters predictive of conception rates in Bos indicus cows submitted to timed-artificial insemination-partial results. Animal Reproduction, v. 15, n. 3, p. 463, jul./sept. 2018. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal; Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 6 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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