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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
08/03/1996 |
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Data da última atualização: |
27/06/2023 |
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Autoria: |
RIMSTAD, E.; EAST, N.; DeROCK, E.; HIGGINS, J.; PEDERSEN, N. C. |
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Título: |
Detection of antibodies to caprine arthritis-encephalitis virus using recombinant GAG proteins. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Archives of Virology, v. 134, n. 3/4, p. 345-356, 1994. |
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DOI: |
10.1007/BF01310572. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The coding sequences of the core proteins p17 and p28 of caprine arthritis-encephalitis virus (CAEV) were amplified using the polymerase chain reaction and cloned into the plasmid expression vector p-GEX-2T. Both p17 and p28 were expressed as fusion proteins with glutathione S-transferase. The recombinant proteins were affinity purified from induced bacterial lysates using glutathione-agarose beads. The purified proteins were used in an enzyme-linked immunosorbent assay (ELISA) to detect antibodies against CAEV in goat sera and milk samples. Three different ELISA tests were developed based on p17, p28 or the combination of these two recombinant proteins (p17 + p28). A comparison was made to an ELISA based on purified whole virus particles and to agar immunodiffusion test (AGID). Sera with conflicting results in the different ELISA tests were examined by Western blotting. There was a high correlation between the ELISA tests based on p17 + p28 recombinant proteins and whole virus ELISA, with an estimated kappa value of 0.92. Only 72-75% of the sera that tested positive in these two ELISA tests were positive in AGID. Antibodies to CAEV were detected in significantly more animals when serum samples were tested compared to milk samples. Based on the time and materials required to prepare the reagents, the recombinant based ELISA test was less expensive than the whole virus ELISA. |
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Palavras-Chave: |
Artrite encefalite; Base Sequence; Cloning Molecular; Gene Products gag; Milk microbiology. |
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Thesagro: |
Caprino; Doença; Escherichia Coli. |
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Thesaurus Nal: |
Bacterial infections; Caprine arthritis encephalitis virus; Caprine arthritis-encephalitis; DNA primers; Enzyme-linked immunosorbent assay; Goat diseases; Immunology; Lentivirus; Microbiology; Polymerase chain reaction; Recombinant fusion proteins; Viral antibodies. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02650naa a2200421 a 4500
001 1523529
005 2023-06-27
008 1994 bl uuuu u00u1 u #d
024 7 $a10.1007/BF01310572.$2DOI
100 1 $aRIMSTAD, E.
245 $aDetection of antibodies to caprine arthritis-encephalitis virus using recombinant GAG proteins.$h[electronic resource]
260 $c1994
520 $aAbstract: The coding sequences of the core proteins p17 and p28 of caprine arthritis-encephalitis virus (CAEV) were amplified using the polymerase chain reaction and cloned into the plasmid expression vector p-GEX-2T. Both p17 and p28 were expressed as fusion proteins with glutathione S-transferase. The recombinant proteins were affinity purified from induced bacterial lysates using glutathione-agarose beads. The purified proteins were used in an enzyme-linked immunosorbent assay (ELISA) to detect antibodies against CAEV in goat sera and milk samples. Three different ELISA tests were developed based on p17, p28 or the combination of these two recombinant proteins (p17 + p28). A comparison was made to an ELISA based on purified whole virus particles and to agar immunodiffusion test (AGID). Sera with conflicting results in the different ELISA tests were examined by Western blotting. There was a high correlation between the ELISA tests based on p17 + p28 recombinant proteins and whole virus ELISA, with an estimated kappa value of 0.92. Only 72-75% of the sera that tested positive in these two ELISA tests were positive in AGID. Antibodies to CAEV were detected in significantly more animals when serum samples were tested compared to milk samples. Based on the time and materials required to prepare the reagents, the recombinant based ELISA test was less expensive than the whole virus ELISA.
650 $aBacterial infections
650 $aCaprine arthritis encephalitis virus
650 $aCaprine arthritis-encephalitis
650 $aDNA primers
650 $aEnzyme-linked immunosorbent assay
650 $aGoat diseases
650 $aImmunology
650 $aLentivirus
650 $aMicrobiology
650 $aPolymerase chain reaction
650 $aRecombinant fusion proteins
650 $aViral antibodies
650 $aCaprino
650 $aDoença
650 $aEscherichia Coli
653 $aArtrite encefalite
653 $aBase Sequence
653 $aCloning Molecular
653 $aGene Products gag
653 $aMilk microbiology
700 1 $aEAST, N.
700 1 $aDeROCK, E.
700 1 $aHIGGINS, J.
700 1 $aPEDERSEN, N. C.
773 $tArchives of Virology$gv. 134, n. 3/4, p. 345-356, 1994.
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| 6. |  | SANTOS, M. I. dos; SOUZA, R. da S.; WANDER, A. E.; CUNHA, C. A.; FERNANDES, S. de M. Estimação da equação de demanda brasileira por importação de arroz da Argentina. In: CONGRESSO SOCIEDADE BRASILEIRA DE ECONOMIA, ADMINISTRAÇÃO E SOCIOLOGIA RURAL, 47., 2009, Porto Alegre. Desenvolvimento rural e sistemas agroalimentares: os agronegócios no contexto de integração das nações: anais. Porto Alegre: Sociedade Brasileira de Economia, Administração e Sociologia Rural, 2009. 15 p.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
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