|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
21/03/1996 |
|
Data da última atualização: |
24/05/2023 |
|
Autoria: |
RITAR, A. J.; BALL, P. D.; O'MAY, P. J. |
|
Título: |
Examination of methods for the deep freezing of goat semen. |
|
Ano de publicação: |
1990 |
|
Fonte/Imprenta: |
Reproduction Fertility and Development, v. 2, n. 1, p. 27-34, 1990. |
|
DOI: |
10.1071/rd9900027. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: In four experiments, freezing of goat semen in straws was examined and compared with conventional pellet-freezing. The optimum freezing regime was attained by holding straws in vapour 4 cm above liquid nitrogen for 30 s or longer, followed by plunging into liquid nitrogen. Straws exposed to vapour for only 10 s did not cool sufficiently before plunging, and cell viability was seriously impaired. Initiation of ice crystallization and the pattern of cooling of semen depended on size of straws and the type of rack used to hold straws in vapour during freezing. Nevertheless, semen was equally well stored in straws of 0.25 mL and 0.50 mL capacity and straws were most efficiently filled at 30 degrees C. Modified Tris media were used to dilute semen at rates (semen: diluent) of 1:2, 1:5, 1:11 and 1:23. Spermatozoa survived best in hypertonic diluents and deteriorated during post-thawing incubation in media of low tonicity, whilst the optimum diluent depended on rate of extension before freezing. The cooling curves differed markedly for semen frozen in straws in liquid nitrogen vapour and pellets on dry ice. Viability of spermatozoa was better for the pellet-frozen semen. |
|
Palavras-Chave: |
Congelacao; Diluicao; Osmolar concentration; Semen preservation. |
|
Thesagro: |
Caprino; Reprodução; Sêmen. |
|
Thesaurus Nal: |
Freezing; Goats; Males; Reproduction; Sperm motility; Spermatozoa; Temperature. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02047naa a2200325 a 4500
001 1523381
005 2023-05-24
008 1990 bl uuuu u00u1 u #d
024 7 $a10.1071/rd9900027.$2DOI
100 1 $aRITAR, A. J.
245 $aExamination of methods for the deep freezing of goat semen.$h[electronic resource]
260 $c1990
520 $aAbstract: In four experiments, freezing of goat semen in straws was examined and compared with conventional pellet-freezing. The optimum freezing regime was attained by holding straws in vapour 4 cm above liquid nitrogen for 30 s or longer, followed by plunging into liquid nitrogen. Straws exposed to vapour for only 10 s did not cool sufficiently before plunging, and cell viability was seriously impaired. Initiation of ice crystallization and the pattern of cooling of semen depended on size of straws and the type of rack used to hold straws in vapour during freezing. Nevertheless, semen was equally well stored in straws of 0.25 mL and 0.50 mL capacity and straws were most efficiently filled at 30 degrees C. Modified Tris media were used to dilute semen at rates (semen: diluent) of 1:2, 1:5, 1:11 and 1:23. Spermatozoa survived best in hypertonic diluents and deteriorated during post-thawing incubation in media of low tonicity, whilst the optimum diluent depended on rate of extension before freezing. The cooling curves differed markedly for semen frozen in straws in liquid nitrogen vapour and pellets on dry ice. Viability of spermatozoa was better for the pellet-frozen semen.
650 $aFreezing
650 $aGoats
650 $aMales
650 $aReproduction
650 $aSperm motility
650 $aSpermatozoa
650 $aTemperature
650 $aCaprino
650 $aReprodução
650 $aSêmen
653 $aCongelacao
653 $aDiluicao
653 $aOsmolar concentration
653 $aSemen preservation
700 1 $aBALL, P. D.
700 1 $aO'MAY, P. J.
773 $tReproduction Fertility and Development$gv. 2, n. 1, p. 27-34, 1990.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
