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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
26/02/1996 |
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Data da última atualização: |
23/06/2023 |
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Autoria: |
STEWART, D. J.; McKERN, N. M.; RAMSHAW, J. A. M.; TULLOCH, P. A. |
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Título: |
The effect of dissociation of bacteroides nodosus pili on their efficacy as a protective antigen agaisnt ovine footrot. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Veterinary Microbiology, v. 27, n. 3/4, p. 283-293, May, 1991. |
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DOI: |
10.1016/0378-1135(91)90155-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Previous studies have shown that pili from homologous Bacteroides nodosus provide protective immunity in sheep against footrot, whereas denatured pilin subunits are ineffective. The aim of the present study was to examine whether pili that were dissociated into pilin subunits under less vigorous, non-denaturing treatment conditions, would provide an effective level of protective immunity. Using the techniques of gel permeation chromatography, light scattering and susceptibility to proteolysis as measures of disruption, it was shown that pili were dissociated either by the neutral detergents n-octyl-beta-D-glucopyranoside (NOG) and Tween 80 or by lowering the pH with 1 M phosphoric acid to pH 2.2. Circular dichroic spectra indicated, however that the samples were not denatured by these treatments. Electron microscopic monitoring of detergent dissociated material following exhaustive dialysis showed the presence of protein-detergent micelles and "in-line" aggregates which gave the appearance of short fibres. Within these monitored preparations, there was no evidence of native undissociated pili. Pili dissociated by NOG or acid were tested in protection trials and shown to provide protective immunity, although agglutination titres of serum taken from the vaccinated sheep were significantly lower than those of animals inoculated with intact pili. |
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Palavras-Chave: |
Foot Rot; Pododermatite. |
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Thesagro: |
Doença; Ovino. |
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Thesaurus Nal: |
Bacterial antigens; Bacteroides; Circular dichroism spectroscopy; Disease control; Disease prevention; Gel chromatography; Glucosides; Immunology; Polysorbates; Sheep diseases; Ultrastructure; Vaccination. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02435naa a2200361 a 4500
001 1522917
005 2023-06-23
008 1991 bl uuuu u00u1 u #d
024 7 $a10.1016/0378-1135(91)90155-9$2DOI
100 1 $aSTEWART, D. J.
245 $aThe effect of dissociation of bacteroides nodosus pili on their efficacy as a protective antigen agaisnt ovine footrot.$h[electronic resource]
260 $c1991
520 $aAbstract: Previous studies have shown that pili from homologous Bacteroides nodosus provide protective immunity in sheep against footrot, whereas denatured pilin subunits are ineffective. The aim of the present study was to examine whether pili that were dissociated into pilin subunits under less vigorous, non-denaturing treatment conditions, would provide an effective level of protective immunity. Using the techniques of gel permeation chromatography, light scattering and susceptibility to proteolysis as measures of disruption, it was shown that pili were dissociated either by the neutral detergents n-octyl-beta-D-glucopyranoside (NOG) and Tween 80 or by lowering the pH with 1 M phosphoric acid to pH 2.2. Circular dichroic spectra indicated, however that the samples were not denatured by these treatments. Electron microscopic monitoring of detergent dissociated material following exhaustive dialysis showed the presence of protein-detergent micelles and "in-line" aggregates which gave the appearance of short fibres. Within these monitored preparations, there was no evidence of native undissociated pili. Pili dissociated by NOG or acid were tested in protection trials and shown to provide protective immunity, although agglutination titres of serum taken from the vaccinated sheep were significantly lower than those of animals inoculated with intact pili.
650 $aBacterial antigens
650 $aBacteroides
650 $aCircular dichroism spectroscopy
650 $aDisease control
650 $aDisease prevention
650 $aGel chromatography
650 $aGlucosides
650 $aImmunology
650 $aPolysorbates
650 $aSheep diseases
650 $aUltrastructure
650 $aVaccination
650 $aDoença
650 $aOvino
653 $aFoot Rot
653 $aPododermatite
700 1 $aMcKERN, N. M.
700 1 $aRAMSHAW, J. A. M.
700 1 $aTULLOCH, P. A.
773 $tVeterinary Microbiology$gv. 27, n. 3/4, p. 283-293, May, 1991.
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Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
21/03/2018 |
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Data da última atualização: |
21/03/2018 |
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Autoria: |
ARGENTA, F. F.; KONRADT, G.; BASSUINO, D. M.; CAPRIOLI, R. A.; LAISSE, C. J. M.; BORSANELLI, A. C.; DUTRA, I. S.; DRIEMEIER, D. |
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Afiliação: |
Fernando F. Argenta, Setor de Patologia Veterinária/Departamento de Patologia Clínica Veterinária/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS; Guilherme Konradt, Setor de Patologia Veterinária/Departamento de Patologia Clínica Veterinária/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS; Daniele M. Bassuino, Setor de Patologia Veterinária/Departamento de Patologia Clínica Veterinária/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS; Rafaela A. Caprioli, Setor de Patologia Veterinária/Departamento de Patologia Clínica Veterinária/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS; Cláudio J. M. Laisse, Setor de Patologia Veterinária/Departamento de Patologia Clínica Veterinária/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS; Ana Carolina Borsanelli, Departamento de Apoio Produção e Saúde Animal/Faculdade de Medicina Veterinária de Araçatuba/Universidade Estadual Paulista - Unesp; Iveraldo S. Dutra, Departamento de Apoio Produção e Saúde Animal/Faculdade de Medicina Veterinária de Araçatuba/Universidade Estadual Paulista - Unesp; David Driemeier, Setor de Patologia Veterinária/Departamento de Patologia Clínica Veterinária/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS. |
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Título: |
Surto de botulismo tipo C em equinos no Rio Grande do Sul. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 37, n. 12, p. 1369-1372, dezembro. 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em inglês: Outbreak of type C botulism in horses in Rio Grande do Sul. |
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Conteúdo: |
Botulism in domestic animals is a fatal poisoning caused by ingestion of Clostridium botulinum neurotoxin. This paper describes epidemiological, clinical and laboratorial aspects of a botulism type C outbreak in horses in a farm in Porto Alegre, Rio Grande do Sul. From a total of 20 horses, seven (35%) were affected and died, and one was euthanized after presenting poor prognosis. The horses, which were raised extensively, showed similar clinical signs, that included limited mobility, lethargy, progressive flaccid paralysis, muscle tremors, sweating, ataxia, and frequent falls. Four horses were necropsied and did not show significant macroscopic and microscopic lesions. Brain and spinal cord samples were analyzed by direct immunofluorescence and were negative for rabies. Liver fragments from the four necropsied horses, soil sediment samples, and suspect water from two reservoirs were evaluated by direct exam to verify the botulinum toxin and/or Clostridium botulinum spores presence. In the serum neutralization bioassay in mice, the type C botulinum toxin was detected in one liver sample, at the same time that the soil sediment and water samples were negative in all assays. Based on the epidemiological, clinical and laboratory data the etiological diagnosis of botulism was established by being caused by botulinum toxin type C, and it is suggested that the stagnant water of the dams is the likely source of the toxin. |
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Palavras-Chave: |
Botulinum toxin type C; Botulism type C; Botulismo tipo C; Toxina botulínica tipo C. |
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Thesagro: |
Clostridium botulinum; Eqüino; Intoxicação. |
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Thesaurus NAL: |
Horses; Poisoning. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174273/1/Surto-de-botuliso-tipo-C-em-equinos-no-Rio-Grande-do-Sul.pdf
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Marc: |
LEADER 02437naa a2200325 a 4500
001 2089569
005 2018-03-21
008 2017 bl uuuu u00u1 u #d
100 1 $aARGENTA, F. F.
245 $aSurto de botulismo tipo C em equinos no Rio Grande do Sul.$h[electronic resource]
260 $c2017
500 $aTítulo em inglês: Outbreak of type C botulism in horses in Rio Grande do Sul.
520 $aBotulism in domestic animals is a fatal poisoning caused by ingestion of Clostridium botulinum neurotoxin. This paper describes epidemiological, clinical and laboratorial aspects of a botulism type C outbreak in horses in a farm in Porto Alegre, Rio Grande do Sul. From a total of 20 horses, seven (35%) were affected and died, and one was euthanized after presenting poor prognosis. The horses, which were raised extensively, showed similar clinical signs, that included limited mobility, lethargy, progressive flaccid paralysis, muscle tremors, sweating, ataxia, and frequent falls. Four horses were necropsied and did not show significant macroscopic and microscopic lesions. Brain and spinal cord samples were analyzed by direct immunofluorescence and were negative for rabies. Liver fragments from the four necropsied horses, soil sediment samples, and suspect water from two reservoirs were evaluated by direct exam to verify the botulinum toxin and/or Clostridium botulinum spores presence. In the serum neutralization bioassay in mice, the type C botulinum toxin was detected in one liver sample, at the same time that the soil sediment and water samples were negative in all assays. Based on the epidemiological, clinical and laboratory data the etiological diagnosis of botulism was established by being caused by botulinum toxin type C, and it is suggested that the stagnant water of the dams is the likely source of the toxin.
650 $aHorses
650 $aPoisoning
650 $aClostridium botulinum
650 $aEqüino
650 $aIntoxicação
653 $aBotulinum toxin type C
653 $aBotulism type C
653 $aBotulismo tipo C
653 $aToxina botulínica tipo C
700 1 $aKONRADT, G.
700 1 $aBASSUINO, D. M.
700 1 $aCAPRIOLI, R. A.
700 1 $aLAISSE, C. J. M.
700 1 $aBORSANELLI, A. C.
700 1 $aDUTRA, I. S.
700 1 $aDRIEMEIER, D.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 37, n. 12, p. 1369-1372, dezembro. 2017.
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