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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
26/02/1996 |
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Data da última atualização: |
23/06/2023 |
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Autoria: |
STEWART, D. J.; McKERN, N. M.; RAMSHAW, J. A. M.; TULLOCH, P. A. |
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Título: |
The effect of dissociation of bacteroides nodosus pili on their efficacy as a protective antigen agaisnt ovine footrot. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Veterinary Microbiology, v. 27, n. 3/4, p. 283-293, May, 1991. |
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DOI: |
10.1016/0378-1135(91)90155-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Previous studies have shown that pili from homologous Bacteroides nodosus provide protective immunity in sheep against footrot, whereas denatured pilin subunits are ineffective. The aim of the present study was to examine whether pili that were dissociated into pilin subunits under less vigorous, non-denaturing treatment conditions, would provide an effective level of protective immunity. Using the techniques of gel permeation chromatography, light scattering and susceptibility to proteolysis as measures of disruption, it was shown that pili were dissociated either by the neutral detergents n-octyl-beta-D-glucopyranoside (NOG) and Tween 80 or by lowering the pH with 1 M phosphoric acid to pH 2.2. Circular dichroic spectra indicated, however that the samples were not denatured by these treatments. Electron microscopic monitoring of detergent dissociated material following exhaustive dialysis showed the presence of protein-detergent micelles and "in-line" aggregates which gave the appearance of short fibres. Within these monitored preparations, there was no evidence of native undissociated pili. Pili dissociated by NOG or acid were tested in protection trials and shown to provide protective immunity, although agglutination titres of serum taken from the vaccinated sheep were significantly lower than those of animals inoculated with intact pili. |
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Palavras-Chave: |
Foot Rot; Pododermatite. |
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Thesagro: |
Doença; Ovino. |
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Thesaurus Nal: |
Bacterial antigens; Bacteroides; Circular dichroism spectroscopy; Disease control; Disease prevention; Gel chromatography; Glucosides; Immunology; Polysorbates; Sheep diseases; Ultrastructure; Vaccination. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02435naa a2200361 a 4500
001 1522917
005 2023-06-23
008 1991 bl uuuu u00u1 u #d
024 7 $a10.1016/0378-1135(91)90155-9$2DOI
100 1 $aSTEWART, D. J.
245 $aThe effect of dissociation of bacteroides nodosus pili on their efficacy as a protective antigen agaisnt ovine footrot.$h[electronic resource]
260 $c1991
520 $aAbstract: Previous studies have shown that pili from homologous Bacteroides nodosus provide protective immunity in sheep against footrot, whereas denatured pilin subunits are ineffective. The aim of the present study was to examine whether pili that were dissociated into pilin subunits under less vigorous, non-denaturing treatment conditions, would provide an effective level of protective immunity. Using the techniques of gel permeation chromatography, light scattering and susceptibility to proteolysis as measures of disruption, it was shown that pili were dissociated either by the neutral detergents n-octyl-beta-D-glucopyranoside (NOG) and Tween 80 or by lowering the pH with 1 M phosphoric acid to pH 2.2. Circular dichroic spectra indicated, however that the samples were not denatured by these treatments. Electron microscopic monitoring of detergent dissociated material following exhaustive dialysis showed the presence of protein-detergent micelles and "in-line" aggregates which gave the appearance of short fibres. Within these monitored preparations, there was no evidence of native undissociated pili. Pili dissociated by NOG or acid were tested in protection trials and shown to provide protective immunity, although agglutination titres of serum taken from the vaccinated sheep were significantly lower than those of animals inoculated with intact pili.
650 $aBacterial antigens
650 $aBacteroides
650 $aCircular dichroism spectroscopy
650 $aDisease control
650 $aDisease prevention
650 $aGel chromatography
650 $aGlucosides
650 $aImmunology
650 $aPolysorbates
650 $aSheep diseases
650 $aUltrastructure
650 $aVaccination
650 $aDoença
650 $aOvino
653 $aFoot Rot
653 $aPododermatite
700 1 $aMcKERN, N. M.
700 1 $aRAMSHAW, J. A. M.
700 1 $aTULLOCH, P. A.
773 $tVeterinary Microbiology$gv. 27, n. 3/4, p. 283-293, May, 1991.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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