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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
01/08/2023 |
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Autoria: |
NISWENDER JR.; L. E.; MIDGLEY JUNIOR, A. V.; NALBANDOV, A. V. |
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Título: |
Radioimmunoassay for bovine and ovine luteinizing hormone. |
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Ano de publicação: |
1969 |
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Fonte/Imprenta: |
Endocrinology, v. 84, n. 5, p. 1166-1173, May, 1969. |
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DOI: |
https://doi.org/10.1210/endo-84-5-1166 |
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Idioma: |
Inglês |
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Conteúdo: |
Separate radioimmunoassays for bovine and ovine luteinizing hormone (LH) have been developed. Antiserum against LH was developed in rabbits and purified preparations of bovine and ovine LH were used for radioiodina-tion. Specificity of the assays was demonstrated by comparing radioimmunoassay and bioassay estimates of the LH content of pituitary preparations containing various ratios of the pituitary hormones. Using the bovine LH radioimmuno-assay and 200 μl aliquots of serum, relatively high levels (12?60 mg/ml) of LH were measured in serum from heifers on the day of estrus and measurable levels of LH were present in serum from castrated males (3?5 ng/ml). The ovine LH assay serum obtained from ewes on the day of estrus contained an average of 26 ng/ml, whereas serum obtained during the follicular and luteal phases of the cycle contained approximately 2 ng/ml. The average LH content of serum from castrated ewes was 4.9 ng/ml, whereas the level in castrated males was 20.9 ng/ml. Serum from normal male sheep contained an average of 2 ng/ml, while serum from a hypo-physectomized ewe had no detectable LH (<0.5 ng/ml). |
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Palavras-Chave: |
Biological Assay; Hormonio luteinizante; Iodine Isotopes; Methods; Radiomunoensaio. |
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Thesagro: |
Endocrinologia; Ovino. |
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Thesaurus Nal: |
Ascorbic acid; Castration; Cattle; Estrus; Luteinizing hormone; Pituitary gland; Pregnancy; Radioimmunoassays; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02114naa a2200361 a 4500
001 1522669
005 2023-08-01
008 1969 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1210/endo-84-5-1166$2DOI
100 1 $aNISWENDER JR.
245 $aRadioimmunoassay for bovine and ovine luteinizing hormone.$h[electronic resource]
260 $c1969
520 $aSeparate radioimmunoassays for bovine and ovine luteinizing hormone (LH) have been developed. Antiserum against LH was developed in rabbits and purified preparations of bovine and ovine LH were used for radioiodina-tion. Specificity of the assays was demonstrated by comparing radioimmunoassay and bioassay estimates of the LH content of pituitary preparations containing various ratios of the pituitary hormones. Using the bovine LH radioimmuno-assay and 200 μl aliquots of serum, relatively high levels (12?60 mg/ml) of LH were measured in serum from heifers on the day of estrus and measurable levels of LH were present in serum from castrated males (3?5 ng/ml). The ovine LH assay serum obtained from ewes on the day of estrus contained an average of 26 ng/ml, whereas serum obtained during the follicular and luteal phases of the cycle contained approximately 2 ng/ml. The average LH content of serum from castrated ewes was 4.9 ng/ml, whereas the level in castrated males was 20.9 ng/ml. Serum from normal male sheep contained an average of 2 ng/ml, while serum from a hypo-physectomized ewe had no detectable LH (<0.5 ng/ml).
650 $aAscorbic acid
650 $aCastration
650 $aCattle
650 $aEstrus
650 $aLuteinizing hormone
650 $aPituitary gland
650 $aPregnancy
650 $aRadioimmunoassays
650 $aSheep
650 $aEndocrinologia
650 $aOvino
653 $aBiological Assay
653 $aHormonio luteinizante
653 $aIodine Isotopes
653 $aMethods
653 $aRadiomunoensaio
700 1 $aL. E.
700 1 $aMIDGLEY JUNIOR, A. V.
700 1 $aNALBANDOV, A. V.
773 $tEndocrinology$gv. 84, n. 5, p. 1166-1173, May, 1969.
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Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 5 | |
| 1. |  | CARDOZO FILHO, J. de L.; MONTEIRO, R. do N.; SILVA, L. P. da; BLOCH JUNIOR, C.; STÁBELI, R. G.; CALDERON, L. de A. Identification of a phylloseptin from Amazon Phyllomedusa palliata(Anura: Hylidae) by Maldi TOT/TOF MS. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE TOXINOLOGIA, 11., 2010, Araxá. Toxinas naturais: conhecimento atual e novos desafios. [S.l.]: Sociedade Brasileira de Toxinologia, 2010. Pôster.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 2. | | CARDOZO FILHO, J. de L.; SOARES, A. A.; SILVA, L. P. da; MONTEIRO, J. R. do N.; ALVES, S. C. de L.; SILVA, R. S.; KAYANO, A. M.; BLOCH JUNIOR, C.; STÁBELI, R. G.; CALDERON, L. de A. Identification of peptides from Amazon Leptodactylus knudseni (Anura: Leptodactylidae) by Maldi TOF/TOF MS. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE TOXINOLOGIA, 11., 2010, Araxá. Toxinas naturais: conhecimento atual e novos desafios. [S.l.]: Sociedade Brasileira de Toxinologia, 2010. Pôster.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | MALAQUIAS, A. D. M.; MARQUES, L. E. C.; PEREIRA, S. S.; FERNANDES, C. de F.; MARANHÃO, A. Q.; STABELI, R. G.; FLOREAN, E. O. P. T.; GUEDES, M. I. F.; FERNANDES, C. F. C. A review of plant-based expression systems as a platform for single-domain recombinant antibody production. International Journal of Biological Macromolecules, v. 193, p. 1130?1137, 2021.| Tipo: Artigo em Periódico Indexado |
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| 4. | | PRADO, N. D. R.; SILVA, M. P. da; MORAIS, M. S. S.; KAYANO, A. M.; MOREIRA-DILL, L. S.; LUIZ, M. B.; FULY, A. L.; HUACCA, M. E. F.; FERNANDES, C. de F.; CALDERON, L. A.; ZULIANI, J. P.; SILVA, L. H. P. da; SOARES, A. M.; STABELI, R. G.; FERNANDES, C. F. C. Inhibition of the Myotoxicity Induced by Bothrops jararacussu Venom and Isolated Phospholipases A2 by Specific Camelid Single-Domain Antibody Fragments. PLOS ONE, v. 11, n. 3, March 30, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Rondônia. |
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| 5. | | LUIZ, M. B.; PEREIRA, S. S.; PRADO, N. D. R.; GONÇALVES, N. R.; KAYANO, A. M.; MOREIRA-DILL, L. S.; SOBRINHO, J. C.; ZANCHI, F. B.; FULY, A. L.; FERNANDES, C. de F.; ZULIANI, J. P.; SOARES, A. M.; STABELI, R. G.; FERNANDES, C. F. C. Camelid Single-Domain Antibodies (VHHs) against Crotoxin: A Basis for Developing Modular Building Blocks for the Enhancement of Treatment or Diagnosis of Crotalic Envenoming. Toxins, v. 10, n. 4, art. 142, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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| Registros recuperados : 5 | |
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