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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
13/03/1996 |
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Data da última atualização: |
29/06/2023 |
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Autoria: |
NUNAMAKER, R. A.; ELLIS, J. A.; WIGINGTON, J. G.; MAcLACHLAN, N. J. |
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Título: |
The detection of intracellular bluetongue virus partciles within ovine erythrocytes. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Comparative Biochemistry and Physiology Part A: Physiology, v. 101, n. 3, p. 471-476, 1992. |
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DOI: |
https://doi.org/10.1016/0300-9629(92)90496-D |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: 1. We report here a simplified method for detecting viruses and other antigenic agents in red blood cells (RBCs). Using a nonionic detergent to prepare cytoskeletons, the interior of RBCs can be scanned rapidly using immunoelectron microscopy. 2. In this study, RBCs from bluetongue (BLU) virus-infected sheep were adsorbed directly onto Formvar-coated, gold electron microscope grids. 3. Cytoskeletons were prepared and then probed using a monoclonal antibody to VP 7, a structural BLU-virus protein and Protein-A gold. 4. Of the ca 32,000 RBCs that were examined from BLU virus-infected sheep, 34 (0.106%) contained labelled BLU virus particles. 5. No labelled particles were observed in any of ca 8000 RBCs taken prior to BLU virus inoculation of sheep. 6. If the antigenic BLU virus particles (which may be viral cores) are in fact infectious, this method of sequestration of virus within RBCs could contribute to the prolonged viremia typical of this arboviral disease, which is known to occur concurrently with circulating neutralizing antibody. |
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Thesagro: |
Doença; Língua Azul; Ovino. |
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Thesaurus Nal: |
Antigenic variation; Bluetongue virus; Disease detection; Erythrocytes; Sheep diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01886naa a2200265 a 4500
001 1522604
005 2023-06-29
008 1992 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/0300-9629(92)90496-D$2DOI
100 1 $aNUNAMAKER, R. A.
245 $aThe detection of intracellular bluetongue virus partciles within ovine erythrocytes.$h[electronic resource]
260 $c1992
520 $aAbstract: 1. We report here a simplified method for detecting viruses and other antigenic agents in red blood cells (RBCs). Using a nonionic detergent to prepare cytoskeletons, the interior of RBCs can be scanned rapidly using immunoelectron microscopy. 2. In this study, RBCs from bluetongue (BLU) virus-infected sheep were adsorbed directly onto Formvar-coated, gold electron microscope grids. 3. Cytoskeletons were prepared and then probed using a monoclonal antibody to VP 7, a structural BLU-virus protein and Protein-A gold. 4. Of the ca 32,000 RBCs that were examined from BLU virus-infected sheep, 34 (0.106%) contained labelled BLU virus particles. 5. No labelled particles were observed in any of ca 8000 RBCs taken prior to BLU virus inoculation of sheep. 6. If the antigenic BLU virus particles (which may be viral cores) are in fact infectious, this method of sequestration of virus within RBCs could contribute to the prolonged viremia typical of this arboviral disease, which is known to occur concurrently with circulating neutralizing antibody.
650 $aAntigenic variation
650 $aBluetongue virus
650 $aDisease detection
650 $aErythrocytes
650 $aSheep diseases
650 $aDoença
650 $aLíngua Azul
650 $aOvino
700 1 $aELLIS, J. A.
700 1 $aWIGINGTON, J. G.
700 1 $aMAcLACHLAN, N. J.
773 $tComparative Biochemistry and Physiology Part A: Physiology$gv. 101, n. 3, p. 471-476, 1992.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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