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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
28/06/2023 |
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Autoria: |
GARNIER, D. H.; COTTA, Y.; TERQUI, M. |
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Título: |
Androgen radioimmunoassay in the ram: results of direct plasma testosterone and dehydroepiandrosterone measurement and physiological evaluation. |
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Ano de publicação: |
1978 |
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Fonte/Imprenta: |
Annales de Biologie Animale, Biochimie, Biophysique, v. 18, n. 2A, p. 265-281, 1978. |
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Idioma: |
Inglês |
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Conteúdo: |
Different radioimmunoassays of testosterone (T) dehydroepiandrosterone (DHA) and 5 a-dihydrotestosterone (5 a-DHT) were investigated for ram plasma. Specificity of the antisera, lack of noticeable binding in plasma, very low levels of other androgens allow direct plasma RIA for DHA and T by the double antibody technique. The levels obtained by this simplified method are in agreement with those found after extraction alone, after extraction and celite chromatography and after quantification with a completely different technique such as gas chromatography. The within assay variabilities for T and DHA were 4.7 p. 100 and 4.6 p. 100 respectively but vary with the level of steroid in plasma. The inter assay variabilities of T were 9.5 p. 100 and 3.2 p. 100 for 1.5 and 11.6 ng/ml of plasma respectively. The antiserum for 5 a-DHT have a specificity such that, even after celite chromatography some androgens (5 p-DHT) may interfere. However determinations of 5 u/5 !-DHT amounts are possible. The physiological validations of direct plasma T and DHA RIA were studied in various conditions. The DHA plasma variations are similar to those of T in Ram from birth to puberty, but the levels are lower. DHA plasma levels show a seasonal variation as does testosterone. Variations within 24 hrs of these two androgens were in synchrony. The direct plasma T and DHA assays are useful and inexpensive tools to characterize ram testicular function. |
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Thesagro: |
Fisiologia; Ovino; Reprodução. |
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Thesaurus Nal: |
Physiology; Radioimmunoassays; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02163naa a2200229 a 4500
001 1521851
005 2023-06-28
008 1978 bl uuuu u00u1 u #d
100 1 $aGARNIER, D. H.
245 $aAndrogen radioimmunoassay in the ram$bresults of direct plasma testosterone and dehydroepiandrosterone measurement and physiological evaluation.$h[electronic resource]
260 $c1978
520 $aDifferent radioimmunoassays of testosterone (T) dehydroepiandrosterone (DHA) and 5 a-dihydrotestosterone (5 a-DHT) were investigated for ram plasma. Specificity of the antisera, lack of noticeable binding in plasma, very low levels of other androgens allow direct plasma RIA for DHA and T by the double antibody technique. The levels obtained by this simplified method are in agreement with those found after extraction alone, after extraction and celite chromatography and after quantification with a completely different technique such as gas chromatography. The within assay variabilities for T and DHA were 4.7 p. 100 and 4.6 p. 100 respectively but vary with the level of steroid in plasma. The inter assay variabilities of T were 9.5 p. 100 and 3.2 p. 100 for 1.5 and 11.6 ng/ml of plasma respectively. The antiserum for 5 a-DHT have a specificity such that, even after celite chromatography some androgens (5 p-DHT) may interfere. However determinations of 5 u/5 !-DHT amounts are possible. The physiological validations of direct plasma T and DHA RIA were studied in various conditions. The DHA plasma variations are similar to those of T in Ram from birth to puberty, but the levels are lower. DHA plasma levels show a seasonal variation as does testosterone. Variations within 24 hrs of these two androgens were in synchrony. The direct plasma T and DHA assays are useful and inexpensive tools to characterize ram testicular function.
650 $aPhysiology
650 $aRadioimmunoassays
650 $aReproduction
650 $aSheep
650 $aFisiologia
650 $aOvino
650 $aReprodução
700 1 $aCOTTA, Y.
700 1 $aTERQUI, M.
773 $tAnnales de Biologie Animale, Biochimie, Biophysique$gv. 18, n. 2A, p. 265-281, 1978.
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