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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/02/2008 |
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Data da última atualização: |
21/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MICLO, L.; GIRARDET, J. -M.; EGITO, A. S. do; MOLLÉ, D.; MARTIN, P.; GAILLARD, J. -L. |
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Afiliação: |
LAURENT MICLO, UNITÉ DE RECHERCHE SUR L'ANIMAL ET LES FONCTIONNALITÉS DES PRODUITS ANIMAUX (UR AFPA L'INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA); JEAN-MICHEL GIRARDET, UNITÉ DE RECHERCHE SUR L'ANIMAL ET LES FONCTIONNALITÉS DES PRODUITS ANIMAUX (UR AFPA L'INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA); ANTONIO SILVIO DO EGITO, CNPC; DANIEL MOLLÉ, L'INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE; PATRICE MARTIN, UNITÉ GÉNOMIQUE ET PHYSIOLOGIE DE LA LACTATION (GLP) INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE; JEAN-LUC GAILLARD, UNITÉ DE RECHERCHE SUR L'ANIMAL ET LES FONCTIONNALITÉS DES PRODUITS ANIMAUX (UR AFPA L'INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INRA). |
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Título: |
The primary structure of a low-M(r) multiphosphorylated variant of beta-casein in equine milk. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Proteomics, v. 7, n. 8, p. 1327-1335, Mar. 2007. |
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DOI: |
10.1002/pmic.200600683 |
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Idioma: |
Inglês |
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Conteúdo: |
Highly phosphorylated casein with a low molecular mass was isolated from Haflinger mare's milk by RP-HPLC. It accounts for 4.0% of the casein content. Its mass was determined by LC-ESI-MS before and after treatment by alkaline phosphatase. The molecular mass found for the apo-form (10 591 ± 2 Da) is in agreement with its primary structure, which was established by ESI-MS/MS from tryptic peptides. It appeared that this short protein (94 amino acid residues) is an internally truncated form of the full-length equine -casein (226 residues). This low-Mr variant of equine -casein displays a large deletion (residues 50-181), due to a cryptic splice site usage occurring within exon 7 during the course of primary transcripts processing. The phosphorylation pattern of this equine -casein variant was investigated by LC-ESI-MS and 2-DE. Seven phosphorylation forms were identified with one to seven phosphate groups with pIs ranging between 4.67 and 4.01. The major isoforms carry five and six phosphate groups. |
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Palavras-Chave: |
Equine Milk; Fosforização; High Pressure Liquid; Leite de égua. |
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Thesagro: |
Caseína; Enzima. |
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Thesaurus Nal: |
Alternative splicing; Amino acid sequences; Beta-casein; Chromatography; Food chemistry; Gel electrophoresis; Horses; Mass spectrometry; Peptides; Phosphorylation; Protein isoforms. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02115naa a2200397 a 4500
001 1521814
005 2025-01-21
008 2007 bl uuuu u00u1 u #d
024 7 $a10.1002/pmic.200600683$2DOI
100 1 $aMICLO, L.
245 $aThe primary structure of a low-M(r) multiphosphorylated variant of beta-casein in equine milk.$h[electronic resource]
260 $c2007
520 $aHighly phosphorylated casein with a low molecular mass was isolated from Haflinger mare's milk by RP-HPLC. It accounts for 4.0% of the casein content. Its mass was determined by LC-ESI-MS before and after treatment by alkaline phosphatase. The molecular mass found for the apo-form (10 591 ± 2 Da) is in agreement with its primary structure, which was established by ESI-MS/MS from tryptic peptides. It appeared that this short protein (94 amino acid residues) is an internally truncated form of the full-length equine -casein (226 residues). This low-Mr variant of equine -casein displays a large deletion (residues 50-181), due to a cryptic splice site usage occurring within exon 7 during the course of primary transcripts processing. The phosphorylation pattern of this equine -casein variant was investigated by LC-ESI-MS and 2-DE. Seven phosphorylation forms were identified with one to seven phosphate groups with pIs ranging between 4.67 and 4.01. The major isoforms carry five and six phosphate groups.
650 $aAlternative splicing
650 $aAmino acid sequences
650 $aBeta-casein
650 $aChromatography
650 $aFood chemistry
650 $aGel electrophoresis
650 $aHorses
650 $aMass spectrometry
650 $aPeptides
650 $aPhosphorylation
650 $aProtein isoforms
650 $aCaseína
650 $aEnzima
653 $aEquine Milk
653 $aFosforização
653 $aHigh Pressure Liquid
653 $aLeite de égua
700 1 $aGIRARDET, J. -M.
700 1 $aEGITO, A. S. do
700 1 $aMOLLÉ, D.
700 1 $aMARTIN, P.
700 1 $aGAILLARD, J. -L.
773 $tProteomics$gv. 7, n. 8, p. 1327-1335, Mar. 2007.
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| 3. |  | TOMM, G. O. Manual para cultivo de canola. [Santa Rosa: Camera, 2003]. 22 p. Título alternativo: Indicações para cultivo de canola no Rio Grande do Sul.| Tipo: Folder/Folheto/Cartilha |
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| 12. | | TOMM, G. O. Tecnologia para trigo. In: CUNHA, G. R. da; TROMBINI, M. de F. (Org.). Trigo no Mercosul: coletânea de artigos. Brasília, DF: Embrapa Comunicação para Transferência de Tecnologia; Passo Fundo: Embrapa Trigo, 1999. p. 143-151| Biblioteca(s): Embrapa Trigo. |
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| 16. | | VOSS, M.; TOMM, G. O. Bicicleta adaptada como semeadora. In: REICHERT, L. J.; REIS, A. V. dos.; DEMENECH, C. R. (Ed.). Máquinas para agricultores familiares: ideias, inovações e criações apresentadas na 3ª Mostra de Máquinas e inventos. Brasília, DF: Embrapa, 2015. p. 83.| Tipo: Capítulo em Livro Técnico-Científico |
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