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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
28/06/2023 |
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Autoria: |
HARD, G. C. |
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Título: |
Electron microscopic examination of Corynebacterium ovis. |
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Ano de publicação: |
1969 |
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Fonte/Imprenta: |
Journal of Bacteriology, v. 93, n. 3, p. 1480-1485, Mar. 1969. |
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DOI: |
10.1128/jb.97.3.1480-1485.1969 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Corybacterium ovis (C. pseudotuberculosis) was examined by electron microscopy after being subjected to various methods of fixation. The organism exhibited a fine structure similar to other corynebacterial species in the appearance of its cell wall, plasma membrane, nuclear apparatus, cytoplasmic matrix, wealth and complexity of intracytoplasmic membrane systems, and polyphosphate granules. An outstanding structural feature was the existence of an electron-dense, floccular layer external to the cell wall which both ligroin and acetone-methanol extractions demonstrated to be the previously postulated surface lipid of this organism. The only variations in structure evident between virulent and attenuated strains was a quantitative difference in the thickness and appearance of the surface lipid. The observation of this layer provided a basis for explaining the surface properties of C. ovis, with particular respect to its clumping capacity in suspencion, the waxiness of its growth on solid media, and its ability to grow as a pellicle on suitable liquid media. The variation in the visible amount of surface lipid between the virulent and avirulent strains adequately explained the divergence of these three surface properties between the strains. |
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Palavras-Chave: |
Analysis membranes; Histocytochemistry; Histological Techniques. |
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Thesagro: |
Corynebacterium Ovis. |
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Thesaurus Nal: |
Cell walls; Lipids; Microbiology; Sheep diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01941naa a2200229 a 4500
001 1521308
005 2023-06-28
008 1969 bl uuuu u00u1 u #d
024 7 $a10.1128/jb.97.3.1480-1485.1969$2DOI
100 1 $aHARD, G. C.
245 $aElectron microscopic examination of Corynebacterium ovis.$h[electronic resource]
260 $c1969
520 $aAbstract: Corybacterium ovis (C. pseudotuberculosis) was examined by electron microscopy after being subjected to various methods of fixation. The organism exhibited a fine structure similar to other corynebacterial species in the appearance of its cell wall, plasma membrane, nuclear apparatus, cytoplasmic matrix, wealth and complexity of intracytoplasmic membrane systems, and polyphosphate granules. An outstanding structural feature was the existence of an electron-dense, floccular layer external to the cell wall which both ligroin and acetone-methanol extractions demonstrated to be the previously postulated surface lipid of this organism. The only variations in structure evident between virulent and attenuated strains was a quantitative difference in the thickness and appearance of the surface lipid. The observation of this layer provided a basis for explaining the surface properties of C. ovis, with particular respect to its clumping capacity in suspencion, the waxiness of its growth on solid media, and its ability to grow as a pellicle on suitable liquid media. The variation in the visible amount of surface lipid between the virulent and avirulent strains adequately explained the divergence of these three surface properties between the strains.
650 $aCell walls
650 $aLipids
650 $aMicrobiology
650 $aSheep diseases
650 $aCorynebacterium Ovis
653 $aAnalysis membranes
653 $aHistocytochemistry
653 $aHistological Techniques
773 $tJournal of Bacteriology$gv. 93, n. 3, p. 1480-1485, Mar. 1969.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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