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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
03/01/2024 |
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Autoria: |
CHILLIARD, Y.; DORLEANS, M.; FEHR, P. M. |
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Título: |
Mise en évidence d'une activité lipoprotéine-lipasique dans le tissu adipeux de chèvre: comparaison de trois méthodes d'extraction. |
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Ano de publicação: |
1977 |
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Fonte/Imprenta: |
Annales de Biologie Animale, Biochimie, Biophysique, , v. 17, n. 1, p. 107-122, 1977. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Lipoprotein lipase activity in goat adipose tissue : comparison of three extraction methods. Lipoprotein lipase (L.P.L.) activity is studied in dairy goat omental adipose tissue and three methods of extracting it are compared. 1) in vitro incubation of tissue pieces in a medium containing heparin and goat serum (I.V. method). 2) homogenization in an ammoniacal buffer containing heparin (H.A. method). 3) homogenization of acetone-ether defatted powders in the ammoniacal buffer (E.A. method). These three types of crude extracts show lipolytic activity on « activated » lntralipid triglycerides. The effects of inhibitors and activators indicate that these activities are essentially due to enzyme L.P.L. Lipolytic activity is inhibited by an excess of substrate and has an apparent Km of 0.19 mM of the substrate triglyceride. Reproductibility of the successive steps of L.P.L. activity assay is estimated. Extraction by in vitro tissue incubation (LV, method) is less reproducible than the two other types of extraction (H.A. and E.A. methods). L.P.L. activity obtained by the 3 extraction methods is similar and highly correlated in the 13 tissue samples from 6 goats in different physiological states |
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Thesagro: |
Caprino; Fisiologia; Lactação. |
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Thesaurus Nal: |
Adipose tissue; Goats; Lactation; Lipoprotein lipase; Physiology; Reproduction. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01991naa a2200253 a 4500
001 1520945
005 2024-01-03
008 1977 bl uuuu u00u1 u #d
100 1 $aCHILLIARD, Y.
245 $aMise en évidence d'une activité lipoprotéine-lipasique dans le tissu adipeux de chèvre$bcomparaison de trois méthodes d'extraction.$h[electronic resource]
260 $c1977
520 $aAbstract: Lipoprotein lipase activity in goat adipose tissue : comparison of three extraction methods. Lipoprotein lipase (L.P.L.) activity is studied in dairy goat omental adipose tissue and three methods of extracting it are compared. 1) in vitro incubation of tissue pieces in a medium containing heparin and goat serum (I.V. method). 2) homogenization in an ammoniacal buffer containing heparin (H.A. method). 3) homogenization of acetone-ether defatted powders in the ammoniacal buffer (E.A. method). These three types of crude extracts show lipolytic activity on « activated » lntralipid triglycerides. The effects of inhibitors and activators indicate that these activities are essentially due to enzyme L.P.L. Lipolytic activity is inhibited by an excess of substrate and has an apparent Km of 0.19 mM of the substrate triglyceride. Reproductibility of the successive steps of L.P.L. activity assay is estimated. Extraction by in vitro tissue incubation (LV, method) is less reproducible than the two other types of extraction (H.A. and E.A. methods). L.P.L. activity obtained by the 3 extraction methods is similar and highly correlated in the 13 tissue samples from 6 goats in different physiological states
650 $aAdipose tissue
650 $aGoats
650 $aLactation
650 $aLipoprotein lipase
650 $aPhysiology
650 $aReproduction
650 $aCaprino
650 $aFisiologia
650 $aLactação
700 1 $aDORLEANS, M.
700 1 $aFEHR, P. M.
773 $tAnnales de Biologie Animale, Biochimie, Biophysique,$gv. 17, n. 1, p. 107-122, 1977.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
