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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
22/05/2023 |
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Autoria: |
BASSETT, J. M.; HINKS, N. T. |
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Título: |
Micro-determination of corticosteroids in ovine peripheral plasma: effects of venipuncture, corticotrophin, insulin and glucose. |
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Ano de publicação: |
1969 |
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Fonte/Imprenta: |
The Journal of Endocrinology, v. 44, n. 3, p. 387-403, Jul. 1969. |
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DOI: |
10.1677/joe.0.0440387 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: A sensitive method for the determination of corticosteroids in 0·1 ml. or less of ovine plasma is described. The method uses the steroid-binding properties of corticosteroid-binding globulin (CBG) and gel filtration on small columns of Sephadex G-25 (fine) at 4° for separation of CBG-bound and free steroids. Cortisol was found to be the predominant corticosteroid in ovine plasma and accounts for about 90% of the value determined by this method. The corticosteroid concentration in peripheral plasma of unstressed sheep was in the range 0·1–1·0 μg./100 ml. In untrained animals, venipuncture increased corticosteroid concentration substantially; training reduced the effect. An infusion of cortisol sodium succinate (100 μg. cortisol/min.) increased the plasma corticosteroid level to 9·5 ± 0·49 μg./100 ml. Intravenous infusion of the synthetic adrenocorticotrophic preparation Synacthen at rates of 10 and 20 μg./hr. for 2 hr. increased peripheral corticosteroid concentrations to 8 μg./100 ml. Single i.v. injections of 0·2–0·8 μg. Synacthen also significantly increased peripheral corticosteroid concentrations 7–15 min. later. The injection of 0·05 and 0·1 μg. Synacthen significantly increased the corticosteroid concentration too, but the increase was not significantly greater than that produced by the injection of acidified saline diluent alone. Injection of insulin (0·25 unit/kg. body weight, i.v.) caused a fivefold increase in the corticosteroid concentration 30–60 min. later, in both adult sheep and lambs. Glucose (0·25 g./kg. body weight, i.v.) had no effect on corticosteroid concentration. MenosAbstract: A sensitive method for the determination of corticosteroids in 0·1 ml. or less of ovine plasma is described. The method uses the steroid-binding properties of corticosteroid-binding globulin (CBG) and gel filtration on small columns of Sephadex G-25 (fine) at 4° for separation of CBG-bound and free steroids. Cortisol was found to be the predominant corticosteroid in ovine plasma and accounts for about 90% of the value determined by this method. The corticosteroid concentration in peripheral plasma of unstressed sheep was in the range 0·1–1·0 μg./100 ml. In untrained animals, venipuncture increased corticosteroid concentration substantially; training reduced the effect. An infusion of cortisol sodium succinate (100 μg. cortisol/min.) increased the plasma corticosteroid level to 9·5 ± 0·49 μg./100 ml. Intravenous infusion of the synthetic adrenocorticotrophic preparation Synacthen at rates of 10 and 20 μg./hr. for 2 hr. increased peripheral corticosteroid concentrations to 8 μg./100 ml. Single i.v. injections of 0·2–0·8 μg. Synacthen also significantly increased peripheral corticosteroid concentrations 7–15 min. later. The injection of 0·05 and 0·1 μg. Synacthen significantly increased the corticosteroid concentration too, but the increase was not significantly greater than that produced by the injection of acidified saline diluent alone. Injection of insulin (0·25 unit/kg. body weight, i.v.) caused a fivefold increase in the corticosteroid concentration 30–60 min. l... Mostrar Tudo |
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Palavras-Chave: |
Bloodletting; Hydrocortisone; Transcortin. |
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Thesagro: |
Endocrinologia; Fisiologia; Ovino; Reprodução. |
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Thesaurus Nal: |
Adrenal cortex hormones; Animal physiology; Endocrinology; Gel chromatography; Glucose; Pharmacology; Reproduction; Sheep; Tritium. |
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Categoria do assunto: |
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Marc: |
LEADER 02627naa a2200337 a 4500
001 1520917
005 2023-05-22
008 1969 bl uuuu u00u1 u #d
024 7 $a10.1677/joe.0.0440387$2DOI
100 1 $aBASSETT, J. M.
245 $aMicro-determination of corticosteroids in ovine peripheral plasma$beffects of venipuncture, corticotrophin, insulin and glucose.$h[electronic resource]
260 $c1969
520 $aAbstract: A sensitive method for the determination of corticosteroids in 0·1 ml. or less of ovine plasma is described. The method uses the steroid-binding properties of corticosteroid-binding globulin (CBG) and gel filtration on small columns of Sephadex G-25 (fine) at 4° for separation of CBG-bound and free steroids. Cortisol was found to be the predominant corticosteroid in ovine plasma and accounts for about 90% of the value determined by this method. The corticosteroid concentration in peripheral plasma of unstressed sheep was in the range 0·1–1·0 μg./100 ml. In untrained animals, venipuncture increased corticosteroid concentration substantially; training reduced the effect. An infusion of cortisol sodium succinate (100 μg. cortisol/min.) increased the plasma corticosteroid level to 9·5 ± 0·49 μg./100 ml. Intravenous infusion of the synthetic adrenocorticotrophic preparation Synacthen at rates of 10 and 20 μg./hr. for 2 hr. increased peripheral corticosteroid concentrations to 8 μg./100 ml. Single i.v. injections of 0·2–0·8 μg. Synacthen also significantly increased peripheral corticosteroid concentrations 7–15 min. later. The injection of 0·05 and 0·1 μg. Synacthen significantly increased the corticosteroid concentration too, but the increase was not significantly greater than that produced by the injection of acidified saline diluent alone. Injection of insulin (0·25 unit/kg. body weight, i.v.) caused a fivefold increase in the corticosteroid concentration 30–60 min. later, in both adult sheep and lambs. Glucose (0·25 g./kg. body weight, i.v.) had no effect on corticosteroid concentration.
650 $aAdrenal cortex hormones
650 $aAnimal physiology
650 $aEndocrinology
650 $aGel chromatography
650 $aGlucose
650 $aPharmacology
650 $aReproduction
650 $aSheep
650 $aTritium
650 $aEndocrinologia
650 $aFisiologia
650 $aOvino
650 $aReprodução
653 $aBloodletting
653 $aHydrocortisone
653 $aTranscortin
700 1 $aHINKS, N. T.
773 $tThe Journal of Endocrinology$gv. 44, n. 3, p. 387-403, Jul. 1969.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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