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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
01/08/1992 |
Data da última atualização: |
03/08/2023 |
Autoria: |
HOLSTAD, G. |
Título: |
Corynebacterium pseudotuberculosis infection in goat. I. Evaluation of two serological diagnostic tests. |
Ano de publicação: |
1986 |
Fonte/Imprenta: |
Acta Veterinaria Scandinavica, v. 27, n. 4, p. 575-583, 1986. |
Idioma: |
Inglês |
Conteúdo: |
The bacterial agglutination test (BAT) and the hemolysis inhibition test (HIT) were employed and evaluated. The threshold value between positive and negative antibody titres in BAT and HIT was determined on the basis of titration results for sera from 25 adult goats infected with Corynebacterium pseudotuber-culosis (positive), and sera from 25 adult goats from a herd in which caseous lymphadenitis did not occur (negative). Antibody titres were expressed as logio to the reciprocal value of the highest positive serum dilution in both tests. Positive titre (T) in BAT was stipulated as T ≥ 2.1 and in HIT as T > 0.6. The sensitivity and specificity was 0.96 in both tests in the material used. Twenty-three of the 25 goats infected with G. pseudotuberculosis were positive in both BAT and HIT. The reproducibility in both tests was described by the use of correlation coefficient and coefficient of variation. The values were estimated using duplicate determination of titres of 155 serum samples. Correlation coefficients were 0.87 for BAT and 0.95 for HIT. Coef-ficients of variation were 26.4 % for BAT and 14.1 % for HIT. The coefficient of variation was related to titres. It was highest for BAT at low titres. It was concluded that both tests can be used for seroepidemio-logical investigations of C. pseudotuberculosis infection in goats. |
Palavras-Chave: |
Serologic Tests; Sorologia. |
Thesagro: |
Caprino; Corynebacterium Pseudotuberculosis; Doença; Linfadenite Caseosa. |
Thesaurus Nal: |
Caseous lymphadenitis; Disease diagnosis; Goat diseases; Goats; Immunology. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02110naa a2200253 a 4500 001 1520531 005 2023-08-03 008 1986 bl uuuu u00u1 u #d 100 1 $aHOLSTAD, G. 245 $aCorynebacterium pseudotuberculosis infection in goat. I. Evaluation of two serological diagnostic tests.$h[electronic resource] 260 $c1986 520 $aThe bacterial agglutination test (BAT) and the hemolysis inhibition test (HIT) were employed and evaluated. The threshold value between positive and negative antibody titres in BAT and HIT was determined on the basis of titration results for sera from 25 adult goats infected with Corynebacterium pseudotuber-culosis (positive), and sera from 25 adult goats from a herd in which caseous lymphadenitis did not occur (negative). Antibody titres were expressed as logio to the reciprocal value of the highest positive serum dilution in both tests. Positive titre (T) in BAT was stipulated as T ≥ 2.1 and in HIT as T > 0.6. The sensitivity and specificity was 0.96 in both tests in the material used. Twenty-three of the 25 goats infected with G. pseudotuberculosis were positive in both BAT and HIT. The reproducibility in both tests was described by the use of correlation coefficient and coefficient of variation. The values were estimated using duplicate determination of titres of 155 serum samples. Correlation coefficients were 0.87 for BAT and 0.95 for HIT. Coef-ficients of variation were 26.4 % for BAT and 14.1 % for HIT. The coefficient of variation was related to titres. It was highest for BAT at low titres. It was concluded that both tests can be used for seroepidemio-logical investigations of C. pseudotuberculosis infection in goats. 650 $aCaseous lymphadenitis 650 $aDisease diagnosis 650 $aGoat diseases 650 $aGoats 650 $aImmunology 650 $aCaprino 650 $aCorynebacterium Pseudotuberculosis 650 $aDoença 650 $aLinfadenite Caseosa 653 $aSerologic Tests 653 $aSorologia 773 $tActa Veterinaria Scandinavica$gv. 27, n. 4, p. 575-583, 1986.
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