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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
30/11/2023 |
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Autoria: |
DULIN, A. M.; PAAPE, M. F.; WERGIN, W. P. |
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Título: |
Differentiation and enumeration of somatic cells in goat milk. |
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Ano de publicação: |
1982 |
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Fonte/Imprenta: |
Journal Food Protection, v. 45, n. 5, p. 435-439, 1982. |
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DOI: |
10.4315/0362-028X-45.5.435. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Non-leukocytic cell-like particles commonly observed in goat milk were examined ultrastructurally and cytochemically. Transmission electron microscopy indicated that these particles were generally membrane-bound and anucleate. They contained granular material in the dilated cisternae of the endoplasmic reticulum and homogeneous electron translucent inclusions that resembled lipid. Histochemical and fluorescent staining indicated that the particles contained large amounts of protein, some lipid, but no deoxyribonucleic acid. Several methods routinely used for estimating somatic cell counts in cow milk were compared to determine which one would give accurate estimates of somatic cell counts in goat milk. No significant difference was found (P> .05) among methods which specifically measure deoxyribonucleic acid. These included Membrane Filter-DNA, direct microscopic somatic cell counts using Pyron in Y-methyl green stain, and Fossomatic cell counts. Results of the Wisconsin Mastitis Test did not differ significantly from Fossomatic cell counts. Because Coulter electronic counts and direct microscopic somatic cell counts using Levowitz-Weber stain could not differentiate between the cell-like particles and the actual leukocytes, these methods resulted in elevated cell counts that were highly variable. Results indicate that only those counting methods that are specific for deoxyribonucleic acid can distinguish cell-like particles from somatic cells, and thereby give reliable estimates of somatic cell numbers in goat milk. MenosAbstract: Non-leukocytic cell-like particles commonly observed in goat milk were examined ultrastructurally and cytochemically. Transmission electron microscopy indicated that these particles were generally membrane-bound and anucleate. They contained granular material in the dilated cisternae of the endoplasmic reticulum and homogeneous electron translucent inclusions that resembled lipid. Histochemical and fluorescent staining indicated that the particles contained large amounts of protein, some lipid, but no deoxyribonucleic acid. Several methods routinely used for estimating somatic cell counts in cow milk were compared to determine which one would give accurate estimates of somatic cell counts in goat milk. No significant difference was found (P> .05) among methods which specifically measure deoxyribonucleic acid. These included Membrane Filter-DNA, direct microscopic somatic cell counts using Pyron in Y-methyl green stain, and Fossomatic cell counts. Results of the Wisconsin Mastitis Test did not differ significantly from Fossomatic cell counts. Because Coulter electronic counts and direct microscopic somatic cell counts using Levowitz-Weber stain could not differentiate between the cell-like particles and the actual leukocytes, these methods resulted in elevated cell counts that were highly variable. Results indicate that only those counting methods that are specific for deoxyribonucleic acid can distinguish cell-like particles from somatic cells, and thereby give rel... Mostrar Tudo |
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Palavras-Chave: |
Célula somática; Contagem. |
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Thesagro: |
Cabra; Caprino; Leite. |
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Thesaurus Nal: |
Goat milk; Goats; Somatic cell count. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
null
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
05/01/2016 |
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Data da última atualização: |
21/01/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
AZEVEDO, F. R. de; GUIMARAES, J. A.; ZOUZA, C. C. de; SANTOS, C. A. M. dos; NERE, D. R.; MOURA, E. da S.; AZEVEDO, R.; SOUSA, F. B. de. |
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Afiliação: |
FRANCISCO ROBERTO DE AZEVEDO, UNIVERSIDADE FEDERAL DO CARIRI; JORGE ANDERSON GUIMARAES, CNPH; CLAUDIOMAR CÍCERO DE SOUZA, UNIVERSIDADE FEDERAL DO CARIRI; CÍCERO ANTONIO MARIANO DOS SANTOS, UNIVERSIDADE FEDERAL DO CARIRI; DANIEL RODRIGUES NERE, UNIVERSIDADE FEDERAL DO CARIRI; ERIDIANE DA SILVA MOURA, UNIVERSIDADE FEDERAL DO CARIRI; RAUL AZEVEDO, UNIVERSIDADE VEDERAL DO CARIRI; FLÁVIO BATISTA DE SOUSA, UNIVERSIDADE FEDERAL DO CARIRI. |
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Título: |
Urina humana como atraente natural de Anastrepha spp. (Diptera: Tephritidae) em pomar de goiaba (Psidium guajava L). |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Arquivos do Instituto Biológico, São Paulo, SP, v. 82, p. 1-7, 2015. |
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ISSN: |
1808-1657 |
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Idioma: |
Português |
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Conteúdo: |
Assim, o objetivo deste trabalho foi avaliar a eficiência da urina humana em substituição ao suco de goiaba, a fim de buscar alternativas para o monitoramento e/ou controle de Anastrepha spp. em pequenas propriedades produtoras de goiaba de mesa do Cariri cearense. |
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Palavras-Chave: |
Moscas-das-frutas. |
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Thesagro: |
Inseto; Praga; Psidium Guajava; Uréia. |
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Thesaurus NAL: |
Tephritidae. |
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Categoria do assunto: |
-- |
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Marc: |
null
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Registro original: |
Embrapa Hortaliças (CNPH) |
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