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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
20/06/2023 |
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Autoria: |
HARD, G. C. |
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Título: |
Comparative toxic effect of the surface lipid of Corynebacterium ovis on peritoneal macrophages. |
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Ano de publicação: |
1975 |
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Fonte/Imprenta: |
Infection Immunity, v. 12, n. 6, p. 1439-1449, Dec. 1975. |
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DOI: |
10.1128/iai.12.6.1439-1449.1975 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The postphagocytic effect on mouse, rabbit, and guinea pig peritoneal macrophages of a petrol-ether lipid extract from Corynebacterium ovis (C. pseudotuberculosis) representing the surface coat of the organism external to the cell wall was investigated by examing three parameters of cytotoxicity, viability assayed by dye exclusion, glycolytic activity, and ultrastructural morphology. The viability test demonstrated a lethal effect on normal and immune mouse macrophages but not on those of the rabbit or guinea pig. Measurement of glycolsis indicated a significant degree of cytotoxicity in normal mouse macrophages ingesting lipid, a nonsignificant depression of activity in cells from immune mice, and no alteration in the activities of rabbit and guinea pig macrophages. Electron microscopy demonstrated that C. ovis surface lipid caused acute lethal injury in normal and immune mouse macrophages. The early stages of degeneration were typified by dilatation of the cisternae of rough endoplasmic reticulum, Golgi lamellae, and nuclear envelope, proceeding to focal disruption of various cell membranes, particularly those of the lipidcontaining phagolysosomes and nucleus. In contrast, over the 3-h period of study, no cytotoxic change was evident in rabbit or guinea pig macrophages. The results add further support to previous observations that the surface lipid of C. ovis plays a major role in the pathogenesis of the organism in mice, but they do not explain the guinea pig's marked susceptibility to infection. MenosAbstract: The postphagocytic effect on mouse, rabbit, and guinea pig peritoneal macrophages of a petrol-ether lipid extract from Corynebacterium ovis (C. pseudotuberculosis) representing the surface coat of the organism external to the cell wall was investigated by examing three parameters of cytotoxicity, viability assayed by dye exclusion, glycolytic activity, and ultrastructural morphology. The viability test demonstrated a lethal effect on normal and immune mouse macrophages but not on those of the rabbit or guinea pig. Measurement of glycolsis indicated a significant degree of cytotoxicity in normal mouse macrophages ingesting lipid, a nonsignificant depression of activity in cells from immune mice, and no alteration in the activities of rabbit and guinea pig macrophages. Electron microscopy demonstrated that C. ovis surface lipid caused acute lethal injury in normal and immune mouse macrophages. The early stages of degeneration were typified by dilatation of the cisternae of rough endoplasmic reticulum, Golgi lamellae, and nuclear envelope, proceeding to focal disruption of various cell membranes, particularly those of the lipidcontaining phagolysosomes and nucleus. In contrast, over the 3-h period of study, no cytotoxic change was evident in rabbit or guinea pig macrophages. The results add further support to previous observations that the surface lipid of C. ovis plays a major role in the pathogenesis of the organism in mice, but they do not explain the guinea pig's ... Mostrar Tudo |
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Palavras-Chave: |
Ascitic Fluid; Cell Survival; Cytology; Drug effects; Intradermal Tests. |
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Thesagro: |
Corynebacterium Ovis; Doença; Linfadenite Caseosa. |
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Thesaurus Nal: |
Glycolysis; Mice; Phagocytosis; Rabbits. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02331naa a2200277 a 4500
001 1520418
005 2023-06-20
008 1975 bl uuuu u00u1 u #d
024 7 $a10.1128/iai.12.6.1439-1449.1975$2DOI
100 1 $aHARD, G. C.
245 $aComparative toxic effect of the surface lipid of Corynebacterium ovis on peritoneal macrophages.$h[electronic resource]
260 $c1975
520 $aAbstract: The postphagocytic effect on mouse, rabbit, and guinea pig peritoneal macrophages of a petrol-ether lipid extract from Corynebacterium ovis (C. pseudotuberculosis) representing the surface coat of the organism external to the cell wall was investigated by examing three parameters of cytotoxicity, viability assayed by dye exclusion, glycolytic activity, and ultrastructural morphology. The viability test demonstrated a lethal effect on normal and immune mouse macrophages but not on those of the rabbit or guinea pig. Measurement of glycolsis indicated a significant degree of cytotoxicity in normal mouse macrophages ingesting lipid, a nonsignificant depression of activity in cells from immune mice, and no alteration in the activities of rabbit and guinea pig macrophages. Electron microscopy demonstrated that C. ovis surface lipid caused acute lethal injury in normal and immune mouse macrophages. The early stages of degeneration were typified by dilatation of the cisternae of rough endoplasmic reticulum, Golgi lamellae, and nuclear envelope, proceeding to focal disruption of various cell membranes, particularly those of the lipidcontaining phagolysosomes and nucleus. In contrast, over the 3-h period of study, no cytotoxic change was evident in rabbit or guinea pig macrophages. The results add further support to previous observations that the surface lipid of C. ovis plays a major role in the pathogenesis of the organism in mice, but they do not explain the guinea pig's marked susceptibility to infection.
650 $aGlycolysis
650 $aMice
650 $aPhagocytosis
650 $aRabbits
650 $aCorynebacterium Ovis
650 $aDoença
650 $aLinfadenite Caseosa
653 $aAscitic Fluid
653 $aCell Survival
653 $aCytology
653 $aDrug effects
653 $aIntradermal Tests
773 $tInfection Immunity$gv. 12, n. 6, p. 1439-1449, Dec. 1975.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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