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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
01/08/1992 |
Data da última atualização: |
02/08/2023 |
Autoria: |
COLAS, G. |
Título: |
Effect of initial freezing temperature addtion of glycerol and dilution on the survival and fertilizing ability of deep-frozen ram semen. |
Ano de publicação: |
1975 |
Fonte/Imprenta: |
Journal of Reproduction and Fertility, v. 42, n. 2, p. 277-285, Feb. 1975. |
DOI: |
10.1530/jrf.0.0420277. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The influence oftemperature, addition of glycerol, initial freezing temperature, method of dilution, level of glycerol in the diluted semen, equilibration time and type of diluent on the survival and fertilizing capacity of deep-frozen according to the best conditions was compared with that of "fresh" semen. The addition of glycerol at plus30 degrees C resulted in a highly significant decrease in the mean proportion of motile spermatozoa immediately after thawing compared with the effect of addition at plus 4 degrees C. The immersion of the straws at minus55 degrees C significantly reduced the revival of the spermatozoa compared with initial freezing at lower temperatures. The exposure time to glycerol had no significant effect on the survival of spermatozoa after thawing and incubation, but fertility was significantly higher with 4% than with 2% glycerol. The I. N. R. A. diluent provided better sperm survival and a significantly higher conception rate than did lactose-egg yolk extender. The semen frozen according to the best conditions (about 50% of the samples) had a fertilizing ability similar to that of "fresh" semen when the proportion of motile spermatozoa before, and after 1 or 3 hr of incubation was equal to or above 45, 40 and 30% respectively. |
Palavras-Chave: |
Cell Survival; Drug effects; Hydrogen-ion Concentration; Time Factors. |
Thesagro: |
Lactose; Ovino; Reprodução; Sêmen; Tecnologia. |
Thesaurus Nal: |
Artificial insemination; Egg yolk; Freezing; Glycerol; Pharmacology; Reproduction; Sheep; Spermatozoa. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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14. |  | SA, C. P. de; ANDRADE, F. G. de; ALMEIDA, N. F. de. Estudo da cadeia produtiva da mandioca no Acre. In: CASTRO, A. M. G. de; LIMA, S. M. V.; GOEDERT, W. J.; FREITAS FILHO, A. de; VASCONCELOS, J. R. P. (ed.). Cadeias produtivas e sistemas naturais: prospecção tecnológica. Brasília, DF: Embrapa-SPI: Embrapa-DPD, 1998. cap. 13, p. 321-341.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Acre. |
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