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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
07/07/2025 |
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Autoria: |
BUYS, J.; WEVER, R.; VAN STIGT, R.; RUITENBERG, E. J. |
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Título: |
The killing of newborn larvae of Trichinella spiralis by eosinophil peroxidase in vitro. |
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Ano de publicação: |
1981 |
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Fonte/Imprenta: |
European Journal of Immunology, v. 11, n. 10, p. 843-845, 1981. |
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DOI: |
10.1002/eji.1830111018 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Helminth infections in mammals are characterized by a high level of eosinophils in parasitized tissues and blood, and it has recently been suggested that these cells have a direct parasiticidal effect. Newborn larvae of Trichinella spiralis can be killed within 20 min by incubation at room temperature in a cell-free system, including purified human eosinophil peroxidase (EPO), H2O2 and chloride at pH 5.5. Killing was measured by microscopic observation of the larvae. The larvicidal effect was dependent on each component of the EPO/H2O2/Cl? system and could be prevented by using SO42? instead of Cl?. Killing was totally inhibited by sodium azide and catalase, and substantially by bovine serum albumin, a protein that is an effective scavenger for HOCI. Since larvae could also be killed directly by HOCI under these conditions and EPO is able to oxidize Cl? to hypochlorous acid, it is very likely that the larvicidal effect of the EPO system is due to formation of hypochlorous acid. It is proposed that in vivo, the combination of EPO, which is exocytosed onto the surface of the parasite, and H2O2, which is generated by stimulated eosinophils, is responsible for the larvicidal effect. |
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Palavras-Chave: |
Helminthoses; Nematodeo. |
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Thesagro: |
Imunologia; Mamífero; Parasitologia. |
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Thesaurus Nal: |
Helminths; Immunology; Infection; Larvicides; Parasitology; Trichinella spiralis. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02044naa a2200301 a 4500
001 1519552
005 2025-07-07
008 1981 bl uuuu u00u1 u #d
024 7 $a10.1002/eji.1830111018$2DOI
100 1 $aBUYS, J.
245 $aThe killing of newborn larvae of Trichinella spiralis by eosinophil peroxidase in vitro.$h[electronic resource]
260 $c1981
520 $aAbstract: Helminth infections in mammals are characterized by a high level of eosinophils in parasitized tissues and blood, and it has recently been suggested that these cells have a direct parasiticidal effect. Newborn larvae of Trichinella spiralis can be killed within 20 min by incubation at room temperature in a cell-free system, including purified human eosinophil peroxidase (EPO), H2O2 and chloride at pH 5.5. Killing was measured by microscopic observation of the larvae. The larvicidal effect was dependent on each component of the EPO/H2O2/Cl? system and could be prevented by using SO42? instead of Cl?. Killing was totally inhibited by sodium azide and catalase, and substantially by bovine serum albumin, a protein that is an effective scavenger for HOCI. Since larvae could also be killed directly by HOCI under these conditions and EPO is able to oxidize Cl? to hypochlorous acid, it is very likely that the larvicidal effect of the EPO system is due to formation of hypochlorous acid. It is proposed that in vivo, the combination of EPO, which is exocytosed onto the surface of the parasite, and H2O2, which is generated by stimulated eosinophils, is responsible for the larvicidal effect.
650 $aHelminths
650 $aImmunology
650 $aInfection
650 $aLarvicides
650 $aParasitology
650 $aTrichinella spiralis
650 $aImunologia
650 $aMamífero
650 $aParasitologia
653 $aHelminthoses
653 $aNematodeo
700 1 $aWEVER, R.
700 1 $aVAN STIGT, R.
700 1 $aRUITENBERG, E. J.
773 $tEuropean Journal of Immunology$gv. 11, n. 10, p. 843-845, 1981.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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