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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
30/06/2023 |
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Autoria: |
LINZELL, J. L.; ANNISON, E. F.; FAZAKERLEY, S.; LNG, R. A. |
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Título: |
The incorporation of acetate, stearate and D(-)-B- hydroxybutrate into milk fat by the isolated perfused mammary gland of the goat. |
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Ano de publicação: |
1967 |
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Fonte/Imprenta: |
Biochemistry Journal, v. 104, n. 1, p. 34-42, Jul. 1967. |
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DOI: |
10.1042/bj1040034. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: 1. Mammary glands of lactating goats were perfused for 12·5?15hr. with heparinized whole blood and infused with a substrate mixture of glucose, acetate and amino acids (and sometimes chylomicra) containing either [1-14C]acetate, d(-)-b-hydroxy[1-14C]butyrate or [U-14C]stearate. 2. There was a substantial net uptake of acetate by the glands and transfer of radioactivity into milk fat. Acetate was extensively utilized for the synthesis of milk fatty acids of chain length up to C14 and to a smaller extent for the synthesis of palmitate. 3. There was a small and variable net uptake of stearate and b-hydroxybutyrate and negligible oxidation of these substrates. However, tissue uptake was demonstrated by a substantial fall in specific radioactivity across the glands and an extensive transfer of radioactivity into milk fatty acids. 4. With b-hydroxybutyrate the labelling of milk fat was very similar to that with acetate, but the distribution of radioactivity suggested a cleavage into C2 fragments of about 40%. 5. Labelled stearate gave rise to highly labelled stearate and oleate in the milk fat. Small amounts of radioactivity were detected in stearate of plasma triglycerides and oleate of plasma free fatty acids. 6. In experiments where there was a decline in milk-fat secretion late in perfusion, the milk fatty acids showed a marked decline in the proportion of stearate and oleate and a rise in the proportion of myristate and palmitate. This did not occur in experiments where milk-fat secretion was maintained at a higher level. 7. The present results confirm that there is a large pool of long-chain fatty acids in mammary tissue that can act as an endogenous source of these substrates. MenosAbstract: 1. Mammary glands of lactating goats were perfused for 12·5?15hr. with heparinized whole blood and infused with a substrate mixture of glucose, acetate and amino acids (and sometimes chylomicra) containing either [1-14C]acetate, d(-)-b-hydroxy[1-14C]butyrate or [U-14C]stearate. 2. There was a substantial net uptake of acetate by the glands and transfer of radioactivity into milk fat. Acetate was extensively utilized for the synthesis of milk fatty acids of chain length up to C14 and to a smaller extent for the synthesis of palmitate. 3. There was a small and variable net uptake of stearate and b-hydroxybutyrate and negligible oxidation of these substrates. However, tissue uptake was demonstrated by a substantial fall in specific radioactivity across the glands and an extensive transfer of radioactivity into milk fatty acids. 4. With b-hydroxybutyrate the labelling of milk fat was very similar to that with acetate, but the distribution of radioactivity suggested a cleavage into C2 fragments of about 40%. 5. Labelled stearate gave rise to highly labelled stearate and oleate in the milk fat. Small amounts of radioactivity were detected in stearate of plasma triglycerides and oleate of plasma free fatty acids. 6. In experiments where there was a decline in milk-fat secretion late in perfusion, the milk fatty acids showed a marked decline in the proportion of stearate and oleate and a rise in the proportion of myristate and palmitate. This did not occur in experiments w... Mostrar Tudo |
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Palavras-Chave: |
Carbon Isotopes; Hydroxybutyrates; In Vitro Techniques; Perfusion; Stearic Acids. |
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Thesagro: |
Caprino; Fisiologia; Glândula Mamaria; Reprodução. |
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Thesaurus Nal: |
Fatty acids; Goats; Lipids; Mammary glands; Metabolism; Reproduction. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02712naa a2200349 a 4500
001 1519527
005 2023-06-30
008 1967 bl uuuu u00u1 u #d
024 7 $a10.1042/bj1040034.$2DOI
100 1 $aLINZELL, J. L.
245 $aThe incorporation of acetate, stearate and D(-)-B- hydroxybutrate into milk fat by the isolated perfused mammary gland of the goat.$h[electronic resource]
260 $c1967
520 $aAbstract: 1. Mammary glands of lactating goats were perfused for 12·5?15hr. with heparinized whole blood and infused with a substrate mixture of glucose, acetate and amino acids (and sometimes chylomicra) containing either [1-14C]acetate, d(-)-b-hydroxy[1-14C]butyrate or [U-14C]stearate. 2. There was a substantial net uptake of acetate by the glands and transfer of radioactivity into milk fat. Acetate was extensively utilized for the synthesis of milk fatty acids of chain length up to C14 and to a smaller extent for the synthesis of palmitate. 3. There was a small and variable net uptake of stearate and b-hydroxybutyrate and negligible oxidation of these substrates. However, tissue uptake was demonstrated by a substantial fall in specific radioactivity across the glands and an extensive transfer of radioactivity into milk fatty acids. 4. With b-hydroxybutyrate the labelling of milk fat was very similar to that with acetate, but the distribution of radioactivity suggested a cleavage into C2 fragments of about 40%. 5. Labelled stearate gave rise to highly labelled stearate and oleate in the milk fat. Small amounts of radioactivity were detected in stearate of plasma triglycerides and oleate of plasma free fatty acids. 6. In experiments where there was a decline in milk-fat secretion late in perfusion, the milk fatty acids showed a marked decline in the proportion of stearate and oleate and a rise in the proportion of myristate and palmitate. This did not occur in experiments where milk-fat secretion was maintained at a higher level. 7. The present results confirm that there is a large pool of long-chain fatty acids in mammary tissue that can act as an endogenous source of these substrates.
650 $aFatty acids
650 $aGoats
650 $aLipids
650 $aMammary glands
650 $aMetabolism
650 $aReproduction
650 $aCaprino
650 $aFisiologia
650 $aGlândula Mamaria
650 $aReprodução
653 $aCarbon Isotopes
653 $aHydroxybutyrates
653 $aIn Vitro Techniques
653 $aPerfusion
653 $aStearic Acids
700 1 $aANNISON, E. F.
700 1 $aFAZAKERLEY, S.
700 1 $aLNG, R. A.
773 $tBiochemistry Journal$gv. 104, n. 1, p. 34-42, Jul. 1967.
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| Registros recuperados : 3 | |
| 1. |  | LEDO, A. da S.; JANDEREK, M.; STAATS, E.; SKOGERBOE, D. Pré-condicionamento de acessos de Saccharum para criopreservação. Revista RG News, Brasília, DF, v. 3, n. 2, 2017. p. 157. Edição Especial dos Anais do 3° Simpósio da Rede de Recursos Genéticos Vegetais do Nordeste, Aracaju, out. 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 2. | | LEDO, A. da S.; JENDEREK, M.; SKOGERBOE, D.; STAATS, E.; MACHADO, C. de A.; OLIVEIRA. L. A. R. de. Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 4, p. 514-517, abr. 2018. Notas Científicas.
Título em português: Criopreservação de embriões zigóticos de coqueiro Anão Verde do Brasil de Jiqui.| Biblioteca(s): Embrapa Tabuleiros Costeiros; Embrapa Unidades Centrais. |
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| Registros recuperados : 3 | |
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