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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
08/01/1997 |
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Data da última atualização: |
21/06/2023 |
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Autoria: |
IONAS, G.; NORMAN, N. G.; CLARKE, J. K.; MARSHALL, R. B. |
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Título: |
A study of the heterogeneity of isolates of mycoplasma ovipneumoniae from sheep in New Zealand. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Veterinary Microbiology, v. 29, n. 3/4. p. 339-347, Nov. 1991. |
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DOI: |
10.1016/0378-1135(91)90141-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: To investigate the heterogeneity of Mycoplasma ovipneumoniae, sixty isolates from three sheep on each of twenty farms were examined by restriction endonuclease analysis (REA) and SDS-PAGE. All were found to be different except for three isolates obtained from one farm. The protein and REA patterns of individual isolates were both highly reproducible and remained unchanged following long term passage (approximately 400 generations) in vitro. No plasmids were detected in the twelve strains which were examined and when two isolates were co-cultured in vitro, no genetic interchange, as judged by changes in REA patterns were detected. Since the heterogeneity of M. ovipneumoniae when examined by SDS-PAGE is too great to allow groups to be recognised, it could be advantageous for this purpose if only surface proteins were compared. As a preliminary step to this end we have identified several surface proteins of M. ovipneumoniae and found that some are common to all strains, one surface protein was shared by five of the eight strains examined and another was unique to one strain. This approach has the potential to allow the recognition of grouping of M. ovipneumoniae isolates. |
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Palavras-Chave: |
Analysis; Bacterial Outer Membrane Proteins; Hicoplasmose; Reproducibility of Results. |
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Thesagro: |
Doença Animal; Ovino. |
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Thesaurus Nal: |
Bacterial proteins; Electrophoresis; Gel electrophoresis; Genetic variation; Microbiology; New Zealand; Plasmids; Restriction mapping; Sheep diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02225naa a2200349 a 4500
001 1519501
005 2023-06-21
008 1991 bl uuuu u00u1 u #d
024 7 $a10.1016/0378-1135(91)90141-2$2DOI
100 1 $aIONAS, G.
245 $aA study of the heterogeneity of isolates of mycoplasma ovipneumoniae from sheep in New Zealand.$h[electronic resource]
260 $c1991
520 $aAbstract: To investigate the heterogeneity of Mycoplasma ovipneumoniae, sixty isolates from three sheep on each of twenty farms were examined by restriction endonuclease analysis (REA) and SDS-PAGE. All were found to be different except for three isolates obtained from one farm. The protein and REA patterns of individual isolates were both highly reproducible and remained unchanged following long term passage (approximately 400 generations) in vitro. No plasmids were detected in the twelve strains which were examined and when two isolates were co-cultured in vitro, no genetic interchange, as judged by changes in REA patterns were detected. Since the heterogeneity of M. ovipneumoniae when examined by SDS-PAGE is too great to allow groups to be recognised, it could be advantageous for this purpose if only surface proteins were compared. As a preliminary step to this end we have identified several surface proteins of M. ovipneumoniae and found that some are common to all strains, one surface protein was shared by five of the eight strains examined and another was unique to one strain. This approach has the potential to allow the recognition of grouping of M. ovipneumoniae isolates.
650 $aBacterial proteins
650 $aElectrophoresis
650 $aGel electrophoresis
650 $aGenetic variation
650 $aMicrobiology
650 $aNew Zealand
650 $aPlasmids
650 $aRestriction mapping
650 $aSheep diseases
650 $aDoença Animal
650 $aOvino
653 $aAnalysis
653 $aBacterial Outer Membrane Proteins
653 $aHicoplasmose
653 $aReproducibility of Results
700 1 $aNORMAN, N. G.
700 1 $aCLARKE, J. K.
700 1 $aMARSHALL, R. B.
773 $tVeterinary Microbiology$gv. 29, n. 3/4. p. 339-347, Nov. 1991.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
