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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
14/04/2009 |
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Data da última atualização: |
30/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COELHO, M. R. R.; CARNEIRO, N. P.; MARRIEL, I. E.; SELDIN, L. |
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Afiliação: |
Marcia Reed Rodrigues Coelho, UFRJ; NEWTON PORTILHO CARNEIRO, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS; Lucy Seldin, UFRJ. |
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Título: |
Molecular detection of nifH gene-containing Paenibacillus in the rhizosphere of sorghum (Sorghum bicolor) sown in Cerrado soil. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Letters in Applied Microbiology, Oxford, v. 48, n. 5, p. 611-617, 2009. |
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DOI: |
10.1111/j1472.765X.2009.02578.x |
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Idioma: |
Inglês |
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Conteúdo: |
Aims: To develop a polymerase chain reaction (PCR)-based approach for the detection of nifH gene-containing Paenibacillus in environmental samples. Methods and Results: The primers, nitHPAENf and nitHPAENr, were designed and tested with DNA from: (i) strains of different nitrogen-fixing Paenibacillus species, (ii) strains of other nitrogen-fixing genera and (iii) rhizosphere of sorghum sown in Cerrado soi! amended with either 12 or 120 kg ha-1 of nitrogen fertilizer. All nitrogen-fixing Paenibacillus strains tested and the DNA samples from rhizosphere soil were amplified when these primers were used, generating a 280 bp fragment. When the PCR products obtained from both sorghum rhizospheres were cloned and sequenced, the majority of the clones analysed could be identified as Paenibacillus durus. Moreover, a greater diversity in the nifH sequences could be observed in the rhizosphere treated with a high amount of nitrogen fertilizer. Conclusions: Nitrogen fertilization slightly influenced the structure of the nifH gene-containing Paenibacillus community in sorghum rhizospheres cultivated in Cerrado soil. Significance and Impact of the Study: The PCR detection method developed is adequate to assess the presence of nifH gene-containing Paenibacillus in the environment and can be used in future to determine the ecological role of this group of micro-organisms for the nitrogen input to the plants. |
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Palavras-Chave: |
nifH; PCR; Primers; Sorghum. |
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Thesaurus Nal: |
Paenibacillus. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02125naa a2200229 a 4500
001 1491946
005 2018-05-30
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1111/j1472.765X.2009.02578.x$2DOI
100 1 $aCOELHO, M. R. R.
245 $aMolecular detection of nifH gene-containing Paenibacillus in the rhizosphere of sorghum (Sorghum bicolor) sown in Cerrado soil.$h[electronic resource]
260 $c2009
520 $aAims: To develop a polymerase chain reaction (PCR)-based approach for the detection of nifH gene-containing Paenibacillus in environmental samples. Methods and Results: The primers, nitHPAENf and nitHPAENr, were designed and tested with DNA from: (i) strains of different nitrogen-fixing Paenibacillus species, (ii) strains of other nitrogen-fixing genera and (iii) rhizosphere of sorghum sown in Cerrado soi! amended with either 12 or 120 kg ha-1 of nitrogen fertilizer. All nitrogen-fixing Paenibacillus strains tested and the DNA samples from rhizosphere soil were amplified when these primers were used, generating a 280 bp fragment. When the PCR products obtained from both sorghum rhizospheres were cloned and sequenced, the majority of the clones analysed could be identified as Paenibacillus durus. Moreover, a greater diversity in the nifH sequences could be observed in the rhizosphere treated with a high amount of nitrogen fertilizer. Conclusions: Nitrogen fertilization slightly influenced the structure of the nifH gene-containing Paenibacillus community in sorghum rhizospheres cultivated in Cerrado soil. Significance and Impact of the Study: The PCR detection method developed is adequate to assess the presence of nifH gene-containing Paenibacillus in the environment and can be used in future to determine the ecological role of this group of micro-organisms for the nitrogen input to the plants.
650 $aPaenibacillus
653 $anifH
653 $aPCR
653 $aPrimers
653 $aSorghum
700 1 $aCARNEIRO, N. P.
700 1 $aMARRIEL, I. E.
700 1 $aSELDIN, L.
773 $tLetters in Applied Microbiology, Oxford$gv. 48, n. 5, p. 611-617, 2009.
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