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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
27/10/2008 |
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Data da última atualização: |
24/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FIGUEIREDO, J. E. F.; KALAPOTHAKIS, E.; GOMEZ, M. V.; BRESSAN, W. |
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Afiliação: |
JOSE EDSON FONTES FIGUEIREDO, CNPMS; E. Kalapothakis, UFMG; M. V. Gomez, UFMG; Wellington Bressan, CNPMS. |
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Título: |
Heterologous expression of an insecticidal gene from the armed spider (Phoneutria nigriventer). |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 14, n. 2, p. 274-293, 2008. |
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DOI: |
10.1590/S1678-91992008000200006 |
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Idioma: |
Inglês |
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Conteúdo: |
Insect-pests are global problems that cause severe damage to crop plants, and their control is commonly based on chemical insecticides. However, negative effects of pesticides on the environment and human health emphasize the necessity to develop alternative methods for insect-pest control. In the present study, a gene coding for the insecticidal peptide TX4(6-1) of the Brazilian armed spider (Phoneutria nígriventer) was cloned in fusion with maltose binding protein (MBP) and expressed in Escherichía colí. The affinity purified protein MBP-GlyTX4 was cleaved with the Xa factor and used for a bioassay against Spodoptera frugíperda and rabbit immunization. Five micrograms GlyTX4 protein injected into the hemocoel of larvae and abdominal cavity of adults produced trembling and uncoordinated movements immediately afier injection and ali adult insects died afier 12h. Afier two days, larvae became paralyzed and the epidermal color changed to dark brown. Furthermore, the development stage was prolonged for two weeks. Altematively, si ices of maize leaves were imbibed with 15 micrograms of the recombinant protein cleaved with the Xa factor and used as diet for larvae. In this experiment, ali larvae died in about 30 minutes. Polyclonal antibodies anti-MBP-GlyTX4 were effective for recognizing MBP and GlyTX4 in whole cell extract from E. coli expressing the recombinant protein. |
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Palavras-Chave: |
Bioinsecticide; Heterologous expression. |
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Thesagro: |
Escherichia Coli; Spodoptera Frugiperda. |
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Thesaurus Nal: |
molecular cloning. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02160naa a2200229 a 4500
001 1491408
005 2018-05-24
008 2008 bl uuuu u00u1 u #d
024 7 $a10.1590/S1678-91992008000200006$2DOI
100 1 $aFIGUEIREDO, J. E. F.
245 $aHeterologous expression of an insecticidal gene from the armed spider (Phoneutria nigriventer).$h[electronic resource]
260 $c2008
520 $aInsect-pests are global problems that cause severe damage to crop plants, and their control is commonly based on chemical insecticides. However, negative effects of pesticides on the environment and human health emphasize the necessity to develop alternative methods for insect-pest control. In the present study, a gene coding for the insecticidal peptide TX4(6-1) of the Brazilian armed spider (Phoneutria nígriventer) was cloned in fusion with maltose binding protein (MBP) and expressed in Escherichía colí. The affinity purified protein MBP-GlyTX4 was cleaved with the Xa factor and used for a bioassay against Spodoptera frugíperda and rabbit immunization. Five micrograms GlyTX4 protein injected into the hemocoel of larvae and abdominal cavity of adults produced trembling and uncoordinated movements immediately afier injection and ali adult insects died afier 12h. Afier two days, larvae became paralyzed and the epidermal color changed to dark brown. Furthermore, the development stage was prolonged for two weeks. Altematively, si ices of maize leaves were imbibed with 15 micrograms of the recombinant protein cleaved with the Xa factor and used as diet for larvae. In this experiment, ali larvae died in about 30 minutes. Polyclonal antibodies anti-MBP-GlyTX4 were effective for recognizing MBP and GlyTX4 in whole cell extract from E. coli expressing the recombinant protein.
650 $amolecular cloning
650 $aEscherichia Coli
650 $aSpodoptera Frugiperda
653 $aBioinsecticide
653 $aHeterologous expression
700 1 $aKALAPOTHAKIS, E.
700 1 $aGOMEZ, M. V.
700 1 $aBRESSAN, W.
773 $tJournal of Venomous Animals and Toxins Including Tropical Diseases$gv. 14, n. 2, p. 274-293, 2008.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 68 | |
| 1. |  | FIGUEIREDO, J. E. F.; TEIXEIRA, M. A.; LIMA, G. V. C.; QUINTAO, P. L.; CORREA, J. A.; BRESSAN W.; PINTO, N. F. J.; CASELA, C. R. Atividade antagonista da bactéria endofítica CNPMS22 contra fungos de sementes do milho (Zea mays). In: CONGRESSO NACIONAL DE MILHO E SORGO, 28.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA DO CARTUCHO, 4., 2010, Goiânia. Potencialidades, desafios e sustentabilidade: resumos expandidos... Sete Lagoas: ABMS, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 2. | | FIGUEIREDO, J. E. F.; TEIXEIRA, M. A.; LIMA, G. V. C.; BRESSAN, W.; PINTO, N. F. J. de; CASELA, C. R. Atividade antagonista in vitro de Bacillus subtilis contra fungos fitopatogênicos do milho e sorgo. In: CONGRESSO NACIONAL DE MILHO E SORGO, 28.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA DO CARTUCHO, 4., 2010, Goiânia. Potencialidades, desafios e sustentabilidade: resumos expandidos... Sete Lagoas: ABMS, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 6. | | FIGUEIREDO, J. E. F.; TEIXEIRA, M. A.; LIMA, G. V. C.; SILVA, C. H. C.; LOPES, S. C.; GOMES, E. A.; GUIMARAES, C. T.; LANA, U. G. de P.; FLATSCHART, A. V. F.; BRESSAN, W. Estudo da diversidade ecológica de bactérias endofíticas, por análise molecular, em germoplasma de milho tropicais. In: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, SIRGEALC, 7., 2009, Pucón, Chile. Proceeding... Santiago de Chile: Ministério de Agricultura, Instituto de Investigaciones Agropecuarias, 2009. p. 75-76.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 7. | | FIGUEIREDO, J. E. F.; TEIXEIRA, M. A.; LIMA, G. V. C.; NEVES, N. A.; MARRA, L. M.; SILVA, C. H. C.; LOPES, S. C.; LANA, U. G. de P.; GOMES, E. A.; GRAZIOTTI, P. H.; FLATSCHART, A. V. F.; BRESSAN, W. Identificação molecular da comunidade bacteriana associada à rizosfera de plantas sempre-viva (Syngonanthus elegans var. elegans) endêmica de campos rupestres brasileiros. In: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, SIRGEALC, 7., 2009, Pucón, Chile. Proceeding... Santiago de Chile: Ministério de Agricultura, Instituto de Investigaciones Agropecuarias, 2009. p. 77-78.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 9. | | FIGUEIREDO, J. E. F.; GOMES, E. A.; GUIMARAES, C. T.; LANA, U. G. de P.; TEIXEIRA, M. A.; LIMA, G. V. C.; BRESSAN, W. Molecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.). Brazilian Journal of Microbiology, São Paulo, v. 40, n. 3, p. 522-534, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 17. | | FIGUEIREDO, J. E. F.; DEPAOLI, H. C.; COELHO, V. T. da S.; CASELA, C. R.; FERREIRA, A. da S.; GUIMARAES, C. T.; GOMES, E. A.; BRESSAN, W. Genetic diversity among Colletotrichum sublineolum pathotypes isolated from sorghum (Sorghum bicolor). Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 5, n. 2, p. 304-318, 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 68 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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