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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
21/11/2006 |
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Data da última atualização: |
01/08/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AGUIAR, R. W. S.; MARTINS, E. S.; VALICENTE, F. H.; CARNEIRO, N. P.; BATISTA, A. C.; MELATTI, V. M.; MONNERAT, R. G.; RIBEIRO, B. M. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS. |
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Título: |
A recombinant truncated Cry1Ca protein is toxic to lepidopteran insects and forms large cuboidal crystals in insect cells. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Current Microbiology, New York, v. 53, n. 4, p. 287-292, 2006. |
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DOI: |
10.1007/s00284-005-0502-3 |
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Idioma: |
Inglês |
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Conteúdo: |
A truncated version of the cry1Ca gene from Bacillus thuringiensis was introduced into the genome of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) under the control of two promoters. A recombinant virus (vSyncry1c) was isolated and used to infect insect cells in culture and insect larvae. Structural and ultrastructural analysis of insects infected with vSyncry1C showed the formation of large cuboidal crystals inside the cytoplasm of insect cells in culture and in insect cadavers late in infection. Infected insect cell extracts were analyzed by SDS-PAGE and Western blot and showed the presence of a 65-kDa polypeptide probably corresponding to the protease processed form of the toxin. Bioassays using purified recombinant toxin crystals showed a CL50 of 19.49 ng/ml for 2nd instar A. gemmatalis larvae and 114.1 ng/ml for S. frugiperda. |
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Thesagro: |
Inseto. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01584naa a2200229 a 4500
001 1490381
005 2018-08-01
008 2006 bl uuuu u00u1 u #d
024 7 $a10.1007/s00284-005-0502-3$2DOI
100 1 $aAGUIAR, R. W. S.
245 $aA recombinant truncated Cry1Ca protein is toxic to lepidopteran insects and forms large cuboidal crystals in insect cells.$h[electronic resource]
260 $c2006
520 $aA truncated version of the cry1Ca gene from Bacillus thuringiensis was introduced into the genome of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) under the control of two promoters. A recombinant virus (vSyncry1c) was isolated and used to infect insect cells in culture and insect larvae. Structural and ultrastructural analysis of insects infected with vSyncry1C showed the formation of large cuboidal crystals inside the cytoplasm of insect cells in culture and in insect cadavers late in infection. Infected insect cell extracts were analyzed by SDS-PAGE and Western blot and showed the presence of a 65-kDa polypeptide probably corresponding to the protease processed form of the toxin. Bioassays using purified recombinant toxin crystals showed a CL50 of 19.49 ng/ml for 2nd instar A. gemmatalis larvae and 114.1 ng/ml for S. frugiperda.
650 $aInseto
700 1 $aMARTINS, E. S.
700 1 $aVALICENTE, F. H.
700 1 $aCARNEIRO, N. P.
700 1 $aBATISTA, A. C.
700 1 $aMELATTI, V. M.
700 1 $aMONNERAT, R. G.
700 1 $aRIBEIRO, B. M.
773 $tCurrent Microbiology, New York$gv. 53, n. 4, p. 287-292, 2006.
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