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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
10/01/2001 |
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Data da última atualização: |
08/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FORATO, L. A.; COLNAGO, L. A.; GARRATT, R. C.; LOPES, M. A. |
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Afiliação: |
EMBRAPA-CNPMS. |
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Título: |
Identification of free fatty acids in maize protein bodies and purified a zeins by 13C and 1H nuclear magnetic resonance. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Biochimica et Biophysica Acta, Amsterdam, v. 1543, p. 106-114, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
Zeins, the maize storage proteins, are the most abundant proteins in the corn endosperm, and are synthesized on the rough endoplasmatic reticulum and deposited in discrete organelles called protein bodies. Several authors, using circular dichroism and optical rotatory dispersion, have concluded that these proteins have a high a-helical content in alcoholic solution. In this work we have studied these proteins, within the protein bodies themselves and after extraction from the corn grains with 70% ethanol, using NMR (nuclear magnetic resonance) spectroscopy. We conclusively demonstrate the presence of free fatty acids within both the protein bodies and also in the alcohol extracted a zeins. We present evidence for a direct interaction between the free fatty acids and the a zein proteins within the protein body and suggest possible mechanisms by which such an association has arisen during the evolution of the maize endosperm. |
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Thesagro: |
Biologia Molecular; Bioquímica; Metabolismo; Nitrogênio. |
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Thesaurus Nal: |
biochemistry; metabolism; molecular biology; nitrogen. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01698naa a2200253 a 4500
001 1484469
005 2018-06-08
008 2000 bl uuuu u00u1 u #d
100 1 $aFORATO, L. A.
245 $aIdentification of free fatty acids in maize protein bodies and purified a zeins by 13C and 1H nuclear magnetic resonance.$h[electronic resource]
260 $c2000
520 $aZeins, the maize storage proteins, are the most abundant proteins in the corn endosperm, and are synthesized on the rough endoplasmatic reticulum and deposited in discrete organelles called protein bodies. Several authors, using circular dichroism and optical rotatory dispersion, have concluded that these proteins have a high a-helical content in alcoholic solution. In this work we have studied these proteins, within the protein bodies themselves and after extraction from the corn grains with 70% ethanol, using NMR (nuclear magnetic resonance) spectroscopy. We conclusively demonstrate the presence of free fatty acids within both the protein bodies and also in the alcohol extracted a zeins. We present evidence for a direct interaction between the free fatty acids and the a zein proteins within the protein body and suggest possible mechanisms by which such an association has arisen during the evolution of the maize endosperm.
650 $abiochemistry
650 $ametabolism
650 $amolecular biology
650 $anitrogen
650 $aBiologia Molecular
650 $aBioquímica
650 $aMetabolismo
650 $aNitrogênio
700 1 $aCOLNAGO, L. A.
700 1 $aGARRATT, R. C.
700 1 $aLOPES, M. A.
773 $tBiochimica et Biophysica Acta, Amsterdam$gv. 1543, p. 106-114, 2000.
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Embrapa Milho e Sorgo (CNPMS) |
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| 1. |  | BASTOS, I. T.; BARBOSA, M. H. P.; RESENDE, M. D. V. de; PETERNELLI, L. A.; SILVEIRA, L. C. I. da; DONDA, L. R.; FORTUNATO, A. A. COSTA, P. M. de A.; FIGUEIREDO, I. C. R. de. Avaliação da interação genótipo x ambiente em cana-de-açúcar via modelos mistos. Pesquisa Agropecuária Tropical, Goiânia, v. 37, n. 4, p. 195-203, dez. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Florestas. |
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| 2. | | BASTOS, I. T.; BARBOSA, M. H. P.; RESENDE, M. D. V. de; PEDROZO, C. A.; MELO, C. G.; PETERNELLI, L. A.; COSTA, P. M. de A.; XAVIER, C. V.; BAFFA, C. F. Correlation among predicted genotypic values and adaptability and stability estimates of sugarcane clones in a mixed models context. Scientia Agraria, Curitiba, v. 10, n. 2, p. 111-118, Mar./Abr. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Florestas. |
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